| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MUC12 |
| Uniprot No | Q9UKN1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MUC12重组蛋白的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Cloning and Expression of Recombinant Human MUC12 in Mammalian Cells"*
**作者**: Smith A, et al.
**摘要**: 该研究成功克隆了人源MUC12基因，并在HEK293细胞中实现重组表达。通过Western blot验证了糖基化修饰，为后续功能研究提供工具。
2. **文献名称**: *"Functional Characterization of Recombinant MUC12 in Colorectal Cancer Cell Adhesion"*
**作者**: Lee JH, et al.
**摘要**: 利用重组MUC12蛋白探究其在结直肠癌细胞粘附中的作用，发现其通过调控整合素信号通路抑制肿瘤细胞迁移。
3. **文献名称**: *"Purification and Immunogenicity Analysis of Recombinant MUC12 Extracellular Domain"*
**作者**: Zhang Q, et al.
**摘要**: 开发了基于His标签的亲和层析法纯化MUC12胞外域重组蛋白，证实其在小鼠模型中可诱导特异性抗体反应。
4. **文献名称**: *"Structural Insights into the Tandem Repeat Domain of Recombinant MUC12 by Mass Spectrometry"*
**作者**: Garcia R, et al.
**摘要**: 采用质谱和生物信息学分析重组MUC12的串联重复结构域，揭示其潜在的多肽结合特性及与肿瘤微环境的关联。
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**注意**：以上内容为模拟生成，实际文献可能需要通过PubMed、Web of Science等平台检索关键词(如“MUC12 recombinant protein”)获取。若研究较新或较冷门，相关论文可能有限。
**Background of MUC12 Recombinant Protein**
MUC12 (Mucin 12) is a transmembrane glycoprotein belonging to the mucin family, which plays critical roles in epithelial protection, lubrication, and cell signaling. It is encoded by the *MUC12* gene, located on chromosome 7q22 in humans. Structurally, MUC12 features a large extracellular domain rich in serine, threonine, and proline residues, which undergo extensive O-glycosylation. This modification contributes to its gel-forming properties, enabling it to form a physical barrier on mucosal surfaces. Additionally, MUC12 contains SEA (sea urchin sperm protein, enterokinase, and agrin) modules and epidermal growth factor (EGF)-like domains, suggesting roles in cell adhesion and signaling.
MUC12 is predominantly expressed in epithelial tissues, particularly in the gastrointestinal tract, respiratory system, and reproductive organs. Its overexpression has been linked to pathologies such as cancers (e.g., colorectal, gastric) and inflammatory diseases, where it may promote tumor progression by modulating cell proliferation, metastasis, and immune evasion. In cancer contexts, MUC12 is proposed to interact with signaling pathways like EGFR and Wnt/β-catenin, influencing oncogenic behaviors.
Recombinant MUC12 protein is engineered *in vitro* using expression systems (e.g., mammalian, insect cells) to produce purified, biologically active forms for research. This tool enables studies on MUC12's structure-function relationships, glycosylation patterns, and interactions with ligands or receptors. Applications include investigating its role in mucosal barrier integrity, host-pathogen interactions, and therapeutic targeting. For instance, recombinant MUC12 aids in developing antibodies or inhibitors for cancer immunotherapy or anti-inflammatory strategies. Its production often involves codon optimization and fusion tags to enhance solubility and yield.
Overall, MUC12 recombinant protein serves as a vital resource for elucidating mucin biology and translating findings into clinical interventions for mucin-related disorders.
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