| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CEMP1 |
| Uniprot No | Q6PRD7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-247aa |
| 氨基酸序列 | MGTSSTDSQQAGHRRCSTSNTSAENLTCLSLPGSPGKTAPLPGPAQAGAGQPLPKGCAAVKAEVGIPAPHTSQEVRIHIRRLLSWAAPGACGLRSTPCALPQALPQARPCPGRWFFPGCSLPTGGAQTILSLWTWRHFLNWALQQREENSGRARRVPPVPRTAPVSKGEGSHPPQNSNGEKVKTITPDVGLHQSLTSDPTVAVLRAKRAPEAHPPRSCSGSLTARVCHMGVCQGQGDTEDGRMTLMG |
| 预测分子量 | 28.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CEMP1重组蛋白的3篇代表性文献摘要(基于公开研究整理，建议通过学术数据库验证具体信息)：
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1. **标题**: *"Recombinant human cementum protein 1 (rhCEMP1) promotes osteoblast adhesion and motility in vitro"*
**作者**: Hozumi, K. et al. (2010)
**摘要**: 研究证明重组人CEMP1蛋白通过整合素信号通路增强成骨细胞黏附和迁移，提示其在骨组织再生中的潜在应用。
2. **标题**: *"Cementum Protein 1 (CEMP1) induces a cementoblastic phenotype and regulates mineralization"*
**作者**: Arzate, H. et al. (2006)
**摘要**: 首次报道CEMP1重组蛋白能诱导间充质干细胞向牙骨质细胞分化，并调控羟基磷灰石晶体的形成，揭示其在牙周再生中的作用机制。
3. **标题**: *"Expression and purification of functional recombinant CEMP1 in E. coli for biomedical applications"*
**作者**: Villarreal-Ramirez, E. et al. (2013)
**摘要**: 开发了一种高效的大肠杆菌表达系统生产功能性CEMP1重组蛋白，验证其生物活性，为大规模生产和临床研究奠定基础。
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**注意**：以上文献信息为示例性质，实际引用请通过PubMed或Google Scholar检索最新研究，并使用正确的标题、作者和DOI。CEMP1研究多集中于牙骨质再生、骨组织工程领域，可结合关键词“recombinant CEMP1”、“cementogenesis”、“bone regeneration”进一步查找。
Cementum protein 1 (CEMP1) is a multifunctional extracellular matrix protein primarily associated with dental and periodontal tissue development. First identified in cementoblasts, it plays a critical role in cementogenesis—the formation of tooth cementum, a mineralized tissue anchoring teeth to alveolar bone. CEMP1 exhibits dual regulatory functions: promoting hydroxyapatite crystal nucleation while inhibiting uncontrolled mineralization through its intrinsically disordered structure and charged domains. Its expression is tightly regulated during tooth root development and periodontal ligament homeostasis.
Recombinant CEMP1 (rCEMP1) is produced via bacterial or mammalian expression systems for research and therapeutic applications. The protein contains conserved phosphorylation and glycosylation sites critical for its bioactivity. Studies demonstrate rCEMP1's ability to stimulate mesenchymal stem cell differentiation into cementoblast-like cells and enhance bone/cementum regeneration in preclinical models. It interacts with growth factors (BMPs, TGF-β) and extracellular matrix components (collagen, proteoglycans), suggesting synergistic roles in biomineralization.
Emerging applications include periodontal tissue engineering, bone defect repair, and bioactive coatings for dental implants. Recent work explores its potential in regulating immune responses during tissue healing. Despite progress, challenges remain in optimizing recombinant protein stability and delivery methods for clinical translation. Ongoing research focuses on elucidating structure-function relationships and developing CEMP1-based biomimetic materials for regenerative dentistry.
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