| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGF2BP3 |
| Uniprot No | O00425 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-579aa |
| 氨基酸序列 | MNKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPCDLPLRLLVPTQFVGAIIGKEGATIRNITKQTQSKIDVHRKENAGAAEKSITILSTPEGTSAACKSILEIMHKEAQDIKFTEEIPLKILAHNNFVGRLIGKEGRNLKKIEQDTDTKITISPLQELTLYNPERTITVKGNVETCAKAEEEIMKKIRESYENDIASMNLQAHLIPGLNLNALGLFPPTSGMPPPTSGPPSAMTPPYPQFEQSETETVHLFIPALSVGAIIGKQGQHIKQLSRFAGASIKIAPAEAPDAKVRMVIITGPPEAQFKAQGRIYGKIKEENFVSPKEEVKLEAHIRVPSFAAGRVIGKGGKTVNELQNLSSAEVVVPRDQTPDENDQVVVKITGHFYACQVAQRKIQEILTQVKQHQQQKALQSGPPQSRRK |
| 预测分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGF2BP3重组蛋白的参考文献示例(内容基于公开研究总结，非真实文献)：
---
1. **文献名称**: *IGF2BP3 Recombinant Protein Enhances Tumor Cell Migration via mRNA Stabilization*
**作者**: Huang, L. et al.
**摘要**: 该研究通过大肠杆菌表达系统制备了重组IGF2BP3蛋白，发现其能结合并稳定多种癌基因mRNA(如MYC)，促进结肠癌细胞迁移和侵袭，提示其在肿瘤转移中的潜在作用。
2. **文献名称**: *Structural Characterization of Recombinant IGF2BP3 and Its RNA-Binding Domains*
**作者**: Smith, J.R. et al.
**摘要**: 利用哺乳动物细胞表达系统纯化重组IGF2BP3蛋白，结合X射线晶体学分析其KH结构域与靶RNA的相互作用，揭示了其调控mRNA稳定性的分子机制。
3. **文献名称**: *Development of IGF2BP3 Recombinant Protein as a Diagnostic Biomarker in Pancreatic Cancer*
**作者**: Zhang, Y. et al.
**摘要**: 研究团队开发了高纯度重组IGF2BP3蛋白，用于检测胰腺癌患者血清中的自身抗体水平，证实其作为早期诊断标志物的临床潜力。
4. **文献名称**: *IGF2BP3 Recombinant Protein Modulates miRNA Activity in Gastric Cancer Progression*
**作者**: Lee, S. et al.
**摘要**: 通过体外重组蛋白实验，发现IGF2BP3竞争性结合肿瘤抑制性miRNA(如miR-320a)，解除其对致癌靶基因的抑制，从而驱动胃癌细胞增殖。
---
注：以上文献为示例性内容，实际引用时需以真实发表的论文为准。建议通过PubMed、Web of Science等数据库检索关键词“IGF2BP3 recombinant”或“IGF2BP3 protein expression”获取具体文献。
IGF2BP3 (Insulin-like Growth Factor 2 mRNA-Binding Protein 3) is a member of the conserved IGF2BP family of RNA-binding proteins, which regulate mRNA stability, localization, and translation. It plays critical roles in embryonic development and cellular processes by binding to target transcripts, including insulin-like growth factor 2 (IGF2), MYC, and other oncogenes, through its KH (hnRNP K homology) domains. Dysregulation of IGF2BP3 is strongly linked to cancer progression, where its overexpression correlates with poor prognosis in various malignancies, such as melanoma, pancreatic, and colorectal cancers. It promotes tumor cell proliferation, invasion, and chemoresistance by stabilizing oncogenic mRNAs and enhancing their translation.
Recombinant IGF2BP3 protein is engineered to study its molecular functions and interactions in vitro. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains RNA-binding activity and structural domains essential for its biological role. Researchers utilize it to investigate post-transcriptional gene regulation mechanisms, screen for inhibitors targeting IGF2BP3 in cancer therapy, or develop diagnostic tools. Its purity and functionality are validated through techniques like gel electrophoresis, western blotting, and RNA-binding assays. By enabling precise biochemical and structural studies, recombinant IGF2BP3 serves as a vital tool for unraveling its contributions to both normal physiology and disease pathology.
×
