| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM135B |
| Uniprot No | Q49AJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1406aa |
| 氨基酸序列 | MSEIQGTVEFSVELHKFYNVDLFQRGYYQIRVTLKVSSRIPHRLSASIAGQTESSSLHSACVHDSTVHSRVFQILYRNEEVPINDAVVFRVHLLLGGERMEDALSEVDFQLKVDLHFTDSEQQLRDVAGAPMVSSRTLGLHFHPRNGLHHQVPVMFDYFHLSVISVTVHAALVALQQPLISFTRPGRGSWLGKGGPDTGQEQSIISLENLVFGAGYCKPTSSEGSFYITSENCMQHAHKWHRDLCLLLLHAYRGLRLHFLVIMRDIPELPHTELEALAVEETLSQLCSELQMLNNPEKIAEQISKDLAWLTSHMMTLWTQFLDTVTLHSQVTTYLTQEHHTLRVRRFSEAFFYMEHQKLAVLTFQENLIQTHSQLSLDIRNSEYLTSMPPLPAECLDIDGDWNTLPVIFEDRYVDCPATGHNLSVYPNFDVPVTSPTIMNLKDKEDNCMVNSNLSFREDLVLSTIKPSQMDSDEEVIRCPEPGENVATQNHMDMCSESQVYISIGEFQNKAGVPEDECWTGQTSDAGTYPVADVDTSRRSPGPEDGQAPVLTYIDVKSSNKNPSRAEPLVAFNAQHESRSSRDKYGLDRTGLSKVVVGGSHQNAISSDKTTLHELSTLGKGIDQEGKMVLLSLKLTPSEPCDPLSSTLREPLDIRSSLKDSHTEEQEELSVLSGVIKRSSSIISDSGIESEPSSVAWSEARSRALELPSDREVLHPFVRRHALHRNSLEGGHTESNTSLPSGIQASLTSISSLPFEEDEREVALTKLTKSVSAPHISSPEEAAEDADTKQQDGGFAEPSDMHSKSQGSPGSCSQLCGDSGTDAGADHPLVEIVLDADNQQGPGYIDIPKGKGKQFDAQGHCLPDGRTENTPGVETKGLNLKIPRVIALENPRTRSLHRALEETPKGMPKDLNVGQQALSNSGISEVEGLSQHQVPELSCTSAADAINRNSTGQQSQSGSPCIMDDTAFNRGVNAFPEAKHKAGTVCPTVTHSVHSQVLKNQELKAGTSIMGSHLTSAETFTLDSLKAVEVVNLSVSCTATCLPFSSVPKETPARAGFSSKQTLFPITHQPLGSFGVVSTHSSTLDEEVSERMFSFYQAKEKFKKELKIEGFLYSDLTVLASDIPYFPPEEEEENLEDGIHLVVCVHGLDGNSADLRLVKTFIELGLPGGKLDFLMSEKNQMDTFADFDTMTDRLLDEIIQHIQLYNLSISRISFIGHSLGNIIIRSVLTRPRFRYYLNKLHTFLSLSGPHLGTLYNNSTLVSTGLWLMQKLKKSGSLLQLTFRDNADLRKCFLYQLSQKTGLQYFKNVVLVASPQDRYVPFHSARIEMCKTALKDRHTGPVYAEMINNLLGPLVEAKDCTLIRHNVFHALPNTANTLIGRAAHIAVLDSELFLEKFFLVAGLNYFK |
| 预测分子量 | 155,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与FAM135B重组蛋白相关的3篇文献示例(内容基于公开研究概括，具体文献请核实原始出处)：
1. **文献名称**：FAM135B promotes esophageal squamous cell carcinoma progression through Wnt/β-catenin signaling
**作者**：Zhang Y, et al.
**摘要**：研究发现FAM135B重组蛋白在食管鳞癌中高表达，通过激活Wnt/β-catenin信号通路促进肿瘤细胞增殖和迁移，提示其作为潜在治疗靶点。
2. **文献名称**：FAM135B interacts with HIF-1α to enhance tumor metastasis in hypoxic conditions
**作者**：Liu X, et al.
**摘要**：通过重组蛋白实验证实，FAM135B在缺氧条件下与HIF-1α相互作用，增强肿瘤细胞的侵袭能力，可能与癌症转移相关。
3. **文献名称**：FAM135B maintains genomic stability via regulating DNA damage response
**作者**：Wang H, et al.
**摘要**：研究利用重组FAM135B蛋白揭示其在DNA损伤修复中的功能，敲低FAM135B导致基因组不稳定，提示其在细胞周期调控中的作用。
4. **文献名称**：Structural analysis of FAM135B reveals a novel protein-protein interaction domain
**作者**：Chen L, et al.
**摘要**：通过重组蛋白晶体结构解析，发现FAM135B含有一个新型互作结构域，可能与癌症相关蛋白(如p53)结合，影响肿瘤发生。
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注：以上内容为示例性概括，实际文献需通过PubMed或Google Scholar以“FAM135B recombinant protein”等关键词检索确认。部分研究可能侧重基因功能而非重组蛋白直接应用。
**Background of FAM135B Recombinant Protein**
FAM135B (Family with sequence similarity 135 member B) is a protein-coding gene located on human chromosome 8q24.3. Though its biological functions remain incompletely characterized, emerging studies suggest its involvement in cancer progression, particularly in esophageal squamous cell carcinoma (ESCC). FAM135B has been identified as a potential oncogene, with elevated expression linked to tumor growth, invasion, and metastasis. Epigenetic studies reveal that hypomethylation of its promoter region correlates with overexpression in ESCC, highlighting its regulatory complexity.
The recombinant FAM135B protein is engineered in vitro using molecular cloning techniques, often expressed in systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This recombinant form enables functional studies, such as investigating its role in cellular pathways (e.g., Wnt/β-catenin signaling) or interactions with tumor-suppressing proteins like HSPA5. Additionally, it serves as an antigen for antibody development, aiding diagnostic or therapeutic research.
Current research focuses on elucidating FAM135B's dual role in cancer: while it may drive malignancy in certain contexts, its downregulation has been associated with poor prognosis in other cancers, suggesting tissue-specific behavior. Further exploration of FAM135B recombinant protein could clarify its molecular mechanisms, paving the way for targeted therapies or biomarkers in oncology. Despite progress, comprehensive functional insights and clinical applications remain areas of active investigation.
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