| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THBS3 |
| Uniprot No | P49746 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-956aa |
| 氨基酸序列 | METQELRGALALLLLCFFTSASQDLQVIDLLTVGESRQMVAVAEKIRTALLTAGDIYLLSTFRLPPKQGGVLFGLYSRQDNTRWLEASVVGKINKVLVRYQREDGKVHAVNLQQAGLADGRTHTVLLRLRGPSRPSPALHLYVDCKLGDQHAGLPALAPIPPAEVDGLEIRTGQKAYLRMQGFVESMKIILGGSMARVGALSECPFQGDESIHSAVTNALHSILGEQTKALVTQLTLFNQILVELRDDIRDQVKEMSLIRNTIMECQVCGFHEQRSHCSPNPCFRGVDCMEVYEYPGYRCGPCPPGLQGNGTHCSDINECAHADPCFPGSSCINTMPGFHCEACPRGYKGTQVSGVGIDYARASKQVCNDIDECNDGNNGGCDPNSICTNTVGSFKCGPCRLGFLGNQSQGCLPARTCHSPAHSPCHIHAHCLFERNGAVSCQCNVGWAGNGNVCGTDTDIDGYPDQALPCMDNNKHCKQDNCLLTPNSGQEDADNDGVGDQCDDDADGDGIKNVEDNCRLFPNKDQQNSDTDSFGDACDNCPNVPNNDQKDTDGNGEGDACDNDVDGDGIPNGLDNCPKVPNPLQTDRDEDGVGDACDSCPEMSNPTQTDADSDLVGDVCDTNEDSDGDGHQDTKDNCPQLPNSSQLDSDNDGLGDECDGDDDNDGIPDYVPPGPDNCRLVPNPNQKDSDGNGVGDVCEDDFDNDAVVDPLDVCPESAEVTLTDFRAYQTVVLDPEGDAQIDPNWVVLNQGMEIVQTMNSDPGLAVGYTAFNGVDFEGTFHVNTVTDDDYAGFLFSYQDSGRFYVVMWKQTEQTYWQATPFRAVAQPGLQLKAVTSVSGPGEHLRNALWHTGHTPDQVRLLWTDPRNVGWRDKTSYRWQLLHRPQVGYIRVKLYEGPQLVADSGVIIDTSMRGGRLGVFCFSQENIIWSNLQYRCNDTVPEDFEPFRRQLLQGRV |
| 预测分子量 | 104,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THBS3重组蛋白的3篇参考文献示例(注：以下内容为示例性虚构，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Recombinant THBS3 modulates endothelial cell adhesion and migration through integrin interactions"*
**作者**：Chen L, Wang Y, et al.
**摘要**：该研究成功表达并纯化了重组人THBS3蛋白，发现其通过结合αvβ3整合素调控内皮细胞黏附和迁移，提示其在血管生成中的潜在作用。
2. **文献名称**：*"Functional characterization of recombinant THBS3 in pancreatic cancer progression"*
**作者**：Kim S, Patel R, et al.
**摘要**：研究利用重组THBS3蛋白证实其通过激活TGF-β信号通路促进胰腺癌细胞侵袭，为靶向THBS3的癌症治疗提供依据。
3. **文献名称**：*"Structural insights into the oligomerization of recombinant THBS3 by cryo-EM"*
**作者**：Müller J, Schmidt A, et al.
**摘要**：通过冷冻电镜解析重组THBS3的三维结构，揭示其寡聚化模式及与细胞外基质组分相互作用的分子机制。
---
建议通过 **PubMed** 或 **Google Scholar** 搜索关键词“recombinant THBS3”或“THBS3 protein”获取真实文献。
**Background of THBS3 Recombinant Protein**
Thrombospondin-3 (THBS3) is a member of the thrombospondin family, a group of extracellular matrix glycoproteins involved in cell adhesion, migration, and signaling. The THBS family comprises five members (THBS1-4 and COMP), with THBS3 sharing structural similarities to THBS1 and THBS2. including conserved domains such as N-terminal, oligomerization, epidermal growth factor (EGF)-like, calcium-binding, and C-terminal regions. However, THBS3 lacks the thrombospondin type 1 repeats (TSRs) found in THBS1 and THBS2. which are critical for interactions with cytokines, growth factors, and cell receptors.
THBS3 is primarily expressed in developing tissues and certain adult organs, playing roles in extracellular matrix (ECM) organization, tissue remodeling, and cellular communication. It forms trimers and interacts with components like collagen, integrins, and proteoglycans, influencing cell-matrix adhesion and signaling pathways. Its expression is dysregulated in diseases such as cancer, cardiovascular disorders, and fibrosis, suggesting both pro- and anti-tumorigenic roles depending on context. For example, THBS3 may suppress tumor growth by modulating TGF-β activity or promote metastasis through ECM destabilization.
Recombinant THBS3 protein is produced using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications. It serves as a tool to study THBS3’s molecular mechanisms, receptor interactions, and therapeutic potential. Research highlights its involvement in angiogenesis, inflammation, and tissue repair, though its exact functions remain less characterized compared to other THBS members. Ongoing studies aim to clarify its dual roles in disease and explore applications in regenerative medicine or targeted therapies.
×
