首页 / 产品 / 蛋白 / 信号通路蛋白

Recombinant Human WNT5B protein

  • 中文名: 无翅型MMTV整合位点家族成员5B(WNT5B)重组蛋白
  • 别    名: WNT5B;Protein Wnt-5b
货号: PA1000-9002
Price: ¥询价
数量:
大包装询价

产品详情

纯度>90%SDS-PAGE.
种属Human
靶点WNT5B
Uniprot No Q9H1J7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间18-359aa
氨基酸序列QLL TDANSWWSLA LNPVQRPEMF IIGAQPVCSQ LPGLSPGQRK LCQLYQEHMA YIGEGAKTGI KECQHQFRQR RWNCSTADNA SVFGRVMQIG SRETAFTHAV SAAGVVNAIS RACREGELST CGCSRTARPK DLPRDWLWGG CGDNVEYGYR FAKEFVDARE REKNFAKGSE EQGRVLMNLQ NNEAGRRAVY KMADVACKCH GVSGSCSLKT CWLQLAEFRK VGDRLKEKYD SAAAMRVTRK GRLELVNSRF TQPTPEDLVY VDPSPDYCLR NESTGSLGTQ GRLCNKTSEG MDGCELMCCG RGYNQFKSVQ VERCHCKFHW CCFVRCKKCT EIVDQYICK
预测分子量kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于WNT5B重组蛋白的3篇代表性文献概览(注:文献为虚构示例,实际研究需检索数据库确认):

1. **《重组人WNT5B蛋白在HEK293细胞中的表达及功能验证》**

- 作者:Zhang L et al.

- 摘要:研究通过HEK293细胞系统成功表达并纯化重组人WNT5B蛋白,验证其激活非经典Wnt/Ca²⁺信号通路的能力,并证明其在促进肿瘤细胞迁移中的作用。

2. **《WNT5B重组蛋白通过调控EMT促进结直肠癌侵袭的机制研究》**

- 作者:Kim S et al.

- 摘要:利用大肠杆菌表达系统制备功能性WNT5B重组蛋白,发现其通过诱导上皮-间质转化(EMT)增强结直肠癌细胞侵袭性,提示其作为癌症治疗靶点的潜力。

3. **《重组WNT5B蛋白在干细胞定向分化中的信号调控作用》**

- 作者:Müller R et al.

- 摘要:研究采用哺乳动物细胞表达的重组WNT5B蛋白,揭示其通过抑制经典Wnt/β-catenin通路调控间充质干细胞向成骨细胞分化的分子机制。

建议通过PubMed或Google Scholar搜索关键词“recombinant WNT5B protein”获取真实文献。

背景信息

**Background of WNT5B Recombinant Protein**

WNT5B is a member of the Wnt protein family, a group of evolutionarily conserved secreted glycoproteins that regulate critical developmental and homeostatic processes, including cell proliferation, differentiation, migration, and tissue patterning. Unlike canonical Wnt ligands (e.g., WNT3A) that activate β-catenin-dependent signaling, WNT5B is classified as a non-canonical Wnt protein, primarily engaging β-catenin-independent pathways such as the planar cell polarity (PCP) and calcium-dependent pathways. These pathways influence cell polarity, motility, and tissue organization, playing roles in embryogenesis, stem cell maintenance, and disease progression.

Structurally, WNT5B shares high homology with WNT5A (~85% amino acid identity) and contains conserved lipid-modified domains critical for receptor binding and signal transduction. Its recombinant form is typically produced using mammalian or insect cell expression systems to ensure proper post-translational modifications, such as glycosylation and palmitoylation, which are essential for biological activity.

WNT5B is implicated in diverse physiological and pathological contexts. In development, it contributes to limb bud formation, neural crest cell migration, and organogenesis. Dysregulation of WNT5B is linked to cancers (e.g., breast, colorectal, and pancreatic tumors), where it often promotes invasion, metastasis, and epithelial-mesenchymal transition (EMT). Paradoxically, in some contexts, it may act as a tumor suppressor, highlighting context-dependent roles. Additionally, WNT5B is associated with metabolic disorders, such as obesity and type 2 diabetes, by modulating adipogenesis and insulin signaling.

Recombinant WNT5B protein serves as a vital tool for *in vitro* and *in vivo* studies to dissect its signaling mechanisms, screen therapeutic agents, or model diseases. Its applications extend to regenerative medicine, where it may aid in tissue repair or stem cell differentiation protocols. However, challenges remain in optimizing its stability, delivery, and specificity due to the complexity of Wnt signaling networks and receptor interactions. Ongoing research aims to clarify its dualistic functions and exploit its therapeutic potential.

客户数据及评论

折叠内容

大包装询价

×