| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSIP1 |
| Uniprot No | Q8NA03 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-581aa |
| 氨基酸序列 | MDIIKGNLDG ISKPASNSRI RPGSRSSNAS LEVLSTEPGS FKVDTASNLN SGKEDHSESS NTENRRTSND DKQESCSEKI KLAEEGSDED LDLVQHQIIS ECSDEPKLKE LDSQLQDAIQ KMKKLDKILA KKQRREKEIK KQGLEMRIKL WEEIKSAKYS EAWQSKEEME NTKKFLSLTA VSEETVGPSH EEEDTFSSVF HTQIPPEEYE MQMQKLNKDF TCDVERNESL IKSGKKPFSN TEKIELRGKH NQDFIKRNIE LAKESRNPVV MVDREKKRLV ELLKDLDEKD SGLSSSEGDQ SGWVVPVKGY ELAVTQHQQL AEIDIKLQEL SAASPTISSF SPRLENRNNQ KPDRDGERNM EVTPGEKILR NTKEQRDLHN RLREIDEKLK MMKENVLEST SCLSEEQLKC LLDECILKQK SIIKLSSERK KEDIEDVTPV FPQLSRSIIS KLLNESETKV QKTEVEDADM LESEECEASK GYYLTKALTG HNMSEALVTE AENMKCLQFS KDVIISDTKD YFMSKTLGIG RLKRPSFLDD PLYGISVSLS SEDQHLKLSS PENTIADEQE TKDAAEECKE P |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FSIP1重组蛋白的模拟参考文献示例(请注意文献为虚构，建议通过学术数据库核实真实文献)：
---
1. **文献名称**: *Cloning and functional characterization of FSIP1 recombinant protein in sperm motility*
**作者**: Smith JL, Chen R, et al.
**摘要**: 研究通过基因克隆技术表达FSIP1重组蛋白，发现其与精子纤维鞘结构相互作用，调控鞭毛运动功能，提示其在男性生殖中的潜在作用。
2. **文献名称**: *FSIP1 recombinant protein interacts with AKAP3 in the fibrous sheath of mouse sperm*
**作者**: Tanaka K, Wang Y, et al.
**摘要**: 通过免疫共沉淀和体外结合实验，证明FSIP1重组蛋白与AKAP3存在直接互作，可能参与精子超微结构的组装和信号转导。
3. **文献名称**: *Expression and purification of human FSIP1 recombinant protein for antibody development*
**作者**: Gupta S, Lee M, et al.
**摘要**: 利用大肠杆菌系统高效表达并纯化FSIP1重组蛋白，成功制备多克隆抗体，为后续功能研究和临床检测奠定基础。
4. **文献名称**: *FSIP1 deficiency disrupts sperm capacitation via recombinant protein rescue assays*
**作者**: Zhang H, Liu F, et al.
**摘要**: 通过体外重组蛋白回补实验，证实FSIP1缺失导致精子获能障碍，其重组蛋白可部分恢复钙离子信号通路活性。
---
建议通过 **PubMed** 或 **Google Scholar** 输入关键词“FSIP1 recombinant protein”检索真实文献，或结合具体研究领域(如生殖生物学、蛋白质工程)筛选结果。
**Background of FSIP1 Recombinant Protein**
FSIP1 (Fibrous Sheath-Interacting Protein 1), also known as AKAP-associated sperm fibrous sheath protein, is a structural component predominantly localized to the fibrous sheath of sperm flagella. This sheath, a unique cytoskeletal structure surrounding the axoneme, plays a critical role in sperm motility and metabolic regulation by anchoring signaling proteins and enzymes essential for energy production. FSIP1 is encoded by the *FSIP1* gene and is highly expressed during late spermatogenesis, suggesting its importance in sperm maturation and function.
Studies highlight FSIP1's involvement in fibrous sheath assembly and sperm tail elongation. Mutations or dysregulation of FSIP1 have been linked to morphological abnormalities in sperm, such as shortened or coiled flagella, leading to reduced motility and male infertility. Its interaction with other fibrous sheath proteins, including AKAPs (A-kinase anchoring proteins), underscores its role in organizing signaling complexes that regulate cAMP-dependent pathways, which are vital for sperm capacitation and hyperactivation.
The recombinant FSIP1 protein is engineered to facilitate functional studies, enabling researchers to explore its structural interactions, post-translational modifications, and mechanistic contributions to sperm biology. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the purified protein serves as a tool for antibody development, protein-binding assays, and infertility-related diagnostic research. Additionally, FSIP1 has emerging implications beyond reproduction, with recent studies suggesting potential roles in cancer progression, possibly linked to its structural motifs or signaling partnerships.
Overall, FSIP1 recombinant protein provides a valuable resource for dissecting molecular pathways in spermatogenesis and developing therapeutic strategies for infertility or related disorders.
×
