| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTUS1 |
| Uniprot No | Q9ULD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1270aa |
| 氨基酸序列 | MTDDNSDDKIEDELQTFFTSDKDGNTHAYNPKSPPTQNSSASSVNWNSANPDDMVVDYETDPAVVTGENISLSLQGVEVFGHEKSSSDFISKQVLDMHKDSICQCPALVGTEKPKYLQHSCHSLEAVEGQSVEPSLPFVWKPNDNLNCAGYCDALELNQTFDMTVDKVNCTFISHHAIGKSQSFHTAGSLPPTGRRSGSTSSLSYSTWTSSHSDKTHARETTYDRESFENPQVTPSEAQDMTYTAFSDVVMQSEVFVSDIGNQCACSSGKVTSEYTDGSQQRLVGEKETQALTPVSDGMEVPNDSALQEFFCLSHDESNSEPHSQSSYRHKEMGQNLRETVSYCLIDDECPLMVPAFDKSEAQVLNPEHKVTETEDTQMVSKGKDLGTQNHTSELILSSPPGQKVGSSFGLTWDANDMVISTDKTMCMSTPVLEPTKVTFSVSPIEATEKCKKVEKGNRGLKNIPDSKEAPVNLCKPSLGKSTIKTNTPIGCKVRKTEIISYPRPNFKNVKAKVMSRAVLQPKDAALSKVTPRPQQTSASSPSSVNSRQQTVLSRTPRSDLNADKKAEILINKTHKQQFNKLITSQAVHVTTHSKNASHRVPRTTSAVKSNQEDVDKASSSNSACETGSVSALFQKIKGILPVKMESAECLEMTYVPNIDRISPEKKGEKENGTSMEKQELKQEIMNETFEYGSLFLGSASKTTTTSGRNISKPDSCGLRQIAAPKAKVGPPVSCLRRNSDNRNPSADRAVSPQRIRRVSSSGKPTSLKTAQSSWVNLPRPLPKSKASLKSPALRRTGSTPSIASTHSELSTYSNNSGNAAVIKYEEKPPKPAFQNGSSGSFYLKPLVSRAHVHLMKTPPKGPSRKNLFTALNAVEKSRQKNPRSLCIQPQTAPDALPPEKTLELTQYKTKCENQSGFILQLKQLLACGNTKFEALTVVIQHLLSEREEALKQHKTLSQELVNLRGELVTASTTCEKLEKARNELQTVYEAFVQQHQAEKTERENRLKEFYTREYEKLRDTYIEEAEKYKMQLQEQFDNLNAAHETSKLEIEASHSEKLELLKKAYEASLSEIKKGHEIEKKSLEDLLSEKQESLEKQINDLKSENDALNEKLKSEEQKRRAREKANLKNPQIMYLEQELESLKAVLEIKNEKLHQQDIKLMKMEKLVDNNTALVDKLKRFQQENEELKARMDKHMAISRQLSTEQAVLQESLEKESKVNKRLSMENEELLWKLHNGDLCSPKRSPTSSAIPLQSPRNSGSFPSPSISPR |
| 预测分子量 | 141,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTUS1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *MTUS1/ATIP3a Acts as a Tumor Suppressor in Colorectal Cancer by Downregulating VEGF Signaling*
**作者**: Di Benedetto M et al.
**摘要**: 本研究通过构建重组MTUS1蛋白，发现其过表达可抑制结直肠癌细胞中VEGF信号通路，减少血管生成并抑制肿瘤生长，证实MTUS1通过调控VEGF发挥抑癌作用。
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2. **文献名称**: *Recombinant MTUS1 Protein Inhibits Cell Proliferation via Inducing G1 Phase Arrest in Breast Cancer*
**作者**: Wang Y et al.
**摘要**: 作者通过体外表达MTUS1重组蛋白，证明其能诱导乳腺癌细胞G1期阻滞，抑制Cyclin D1表达，提示MTUS1通过调控细胞周期蛋白抑制肿瘤增殖。
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3. **文献名称**: *MTUS1 Attenuates Angiogenesis by Interacting with HIF-1α in Endothelial Cells*
**作者**: Chen L et al.
**摘要**: 该研究利用重组MTUS1蛋白进行功能实验，发现其与HIF-1α直接结合，抑制低氧诱导的血管内皮细胞迁移和血管生成，为MTUS1的抗血管生成机制提供证据。
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**备注**：上述文献为示例，实际文献需通过PubMed或Web of Science检索确认。建议使用关键词“MTUS1 recombinant protein”或“MTUS1 overexpression”查找最新研究。
MTUS1 (Microtubule Associated Scaffold Protein 1) is a gene encoding a multifunctional protein involved in cellular processes such as cytoskeletal organization, cell migration, proliferation, and apoptosis. Also known as ATIP (AT2 Receptor-Interacting Protein), it was initially identified through its interaction with the angiotensin II type 2 receptor (AT2R), a key player in cardiovascular and renal regulation. The MTUS1 protein family includes several isoforms generated by alternative splicing, with the longest isoform (MTUS1-L/ATIP1) being the most studied. Structurally, it contains conserved domains like an N-terminal microtubule-binding motif, a proline-rich region, and a C-terminal domain mediating protein-protein interactions.
MTUS1 functions as a tumor suppressor, with its downregulation linked to cancer progression in breast, colon, ovarian, and pancreatic cancers. It regulates microtubule dynamics and interacts with signaling pathways (e.g., ERK, PI3K/AKT) to inhibit cell proliferation and promote apoptosis. In cardiovascular contexts, MTUS1/ATIP1 demonstrates protective effects by counteracting pathological vascular remodeling through AT2R-dependent mechanisms.
Recombinant MTUS1 proteins are engineered for functional studies, typically expressed in E. coli or mammalian systems with affinity tags (e.g., His-tag) for purification. These proteins enable in vitro investigations of MTUS1’s interactions (e.g., with microtubules or receptors), its role in cell cycle regulation, and its therapeutic potential. Researchers also utilize MTUS1 recombinant proteins to develop diagnostic tools, as altered MTUS1 expression in tissues or biofluids may serve as a biomarker for cancer or cardiovascular diseases. Current challenges include understanding isoform-specific functions and post-translational modifications influencing its activity.
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