| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | AST2 |
| Uniprot No | P39945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-430aa |
| 氨基酸序列 | MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV KSLVFHSKHG PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT KPIYGEAGIG REYSGVITHV GDNLTNRWNV GDDVYGIYYH PKLAIGALQS SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC LGTALNLLAQ LKEKDQLNTE SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA VLSEHFPDLK DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA RKMFGSMLWS YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK VYSWKNFKEA FSYMATQRAQ GKLIMKVEGF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AST2重组蛋白研究的模拟参考文献示例(请注意，以下内容为假设性示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Expression and Functional Analysis of Recombinant AST2 Protein in Saccharomyces cerevisiae"*
**作者**：Smith J. et al.
**摘要**：本研究成功在大肠杆菌中表达了重组AST2蛋白，并通过亲和层析纯化获得高纯度蛋白。实验证实AST2重组蛋白在酵母细胞中参与应激响应通路，可能通过调节离子平衡增强细胞耐盐性。
2. **文献名称**：*"Structural Characterization of AST2 Recombinant Protein and Its Role in Oxidative Stress Resistance"*
**作者**：Chen L., Wang H.
**摘要**：通过X射线晶体学解析了AST2重组蛋白的三维结构，发现其具有独特的α-螺旋结构域。体外实验表明，该蛋白能够清除活性氧(ROS)，并在哺乳动物细胞模型中减轻氧化损伤。
3. **文献名称**：*"Development of a Novel AST2-Based Fusion Protein for Targeted Drug Delivery"*
**作者**：Kim S. et al.
**摘要**：构建了AST2与肿瘤靶向肽的融合重组蛋白，证实其可通过受体介导的内吞作用特异性进入癌细胞。动物实验显示该蛋白负载化疗药物后显著抑制肿瘤生长，且毒性较低。
4. **文献名称**：*"AST2 Recombinant Protein Enhances Plant Drought Tolerance via ABA Signaling Pathway"*
**作者**：Zhang R. et al.
**摘要**：在拟南芥中过表达AST2重组蛋白，发现其通过激活脱落酸(ABA)信号通路提高气孔关闭效率，从而增强植物抗旱能力。该研究为作物抗逆基因工程提供了新靶点。
**提示**：实际研究中建议通过PubMed、Web of Science或Google Scholar检索最新文献，使用关键词如“AST2 recombinant protein”、“AST2 gene expression”或结合具体研究领域(如疾病模型、植物抗逆等)进行精准查询。
**Background of AST2 Recombinant Protein**
AST2 (Argininosuccinate Synthetase 2) is a key enzyme in the urea cycle and arginine biosynthesis, catalyzing the conversion of citrulline and aspartate to argininosuccinate. While arginine is a semi-essential amino acid critical for protein synthesis, nitric oxide production, and cellular signaling, certain cancers and pathogenic cells exhibit dysregulated arginine metabolism, making AST2 a potential therapeutic target.
Recombinant AST2 protein is engineered using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to produce purified, functional enzyme for research and clinical applications. Its production involves cloning the AST2 gene into expression vectors, optimizing conditions for high-yield synthesis, and employing chromatography-based purification. The recombinant protein retains catalytic activity, enabling studies on arginine metabolism in diseases like hepatocellular carcinoma, melanoma, or urea cycle disorders.
In cancer biology, AST2’s role is dualistic. Some tumors downregulate AST2. creating arginine auxotrophy, a vulnerability exploited by therapies like arginine-depleting agents (e.g., pegylated arginase). Conversely, AST2 overexpression in other cancers may support proliferation, highlighting context-dependent therapeutic strategies. Beyond oncology, recombinant AST2 aids in enzyme replacement therapy research for genetic metabolic disorders and serves as a tool for diagnostic assay development.
Recent advances focus on structural characterization (e.g., crystallography) to design inhibitors or stabilizers, while biopharmaceutical interest lies in fusion proteins or PEGylation to enhance stability and efficacy. Challenges include minimizing immunogenicity and ensuring tissue-specific delivery. Overall, AST2 recombinant protein bridges mechanistic research and translational innovation, offering insights into metabolic reprogramming and precision medicine approaches.
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