| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GTF2E1 |
| Uniprot No | P29083 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-439aa |
| 氨基酸序列 | ADPDVLTEV PAALKRLAKY VIRGFYGIEH ALALDILIRN SCVKEEDMLE LLKFDRKQLR SVLNNLKGDK FIKCRMRVET AADGKTTRHN YYFINYRTLV NVVKYKLDHM RRRIETDERD STNRASFKCP VCSSTFTDLE ANQLFDPMTG TFRCTFCHTE VEEDESAMPK KDARTLLARF NEQIEPIYAL LRETEDVNLA YEILEPEPTE IPALKQSKDH AATTAGAASL AGGHHREAWA TKGPSYEDLY TQNVVINMDD QEDLHRASLE GKSAKERPIW LRESTVQGAY GSEDMKEGGI DMDAFQEREE GHAGPDDNEE VMRALLIHEK KTSSAMAGSV GAAAPVTAAN GSDSESETSE SDDDSPPRPA AVAVHKREED EEEDDEFEEV ADDPIVMVAG RPFSYSEVSQ RPELVAQMTP EEKEAYIAMG QRMFEDLFE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GTF2E1重组蛋白的模拟参考文献示例(仅供学术写作参考，非真实文献)：
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1. **文献名称**：*Expression and Purification of Recombinant Human GTF2E1 for Structural Studies*
**作者**：Smith J, et al.
**摘要**：本研究报道了人源GTF2E1重组蛋白在大肠杆菌中的高效表达与纯化方法。通过优化密码子使用及亲和层析技术，获得了高纯度蛋白，并验证了其与RNA聚合酶II的结合活性，为后续结构功能研究奠定基础。
2. **文献名称**：*Crystal Structure of GTF2E1 Reveals Its Role in Transcription Initiation Complex Assembly*
**作者**：Lee S, Zhang Y, et al.
**摘要**：利用X射线晶体学解析了GTF2E1重组蛋白的三维结构，揭示了其α螺旋结构域与TFIIH的相互作用界面。研究提出GTF2E1通过构象变化调控转录起始复合物的稳定性，为理解转录机制提供结构依据。
3. **文献名称**：*Functional Analysis of GTF2E1 Mutants in a Cellular Model of Transcription Dysregulation*
**作者**：Wang H, et al.
**摘要**：通过构建GTF2E1重组蛋白的致病突变体(如R109C)，发现其导致体外转录活性显著降低，并影响细胞周期进程。该研究支持GTF2E1功能异常与某些遗传性转录缺陷疾病的关联。
4. **文献名称**：*GTF2E1 Interactions with the TFIIH Core in the RNA Polymerase II Pre-Initiation Complex*
**作者**：Garcia-Diaz M, et al.
**摘要**：采用重组GTF2E1蛋白进行体外结合实验，结合冷冻电镜技术，阐明其在RNA聚合酶II预起始复合物中与TFIIH核心组分(如XPB)的动态互作网络，强调了其在转录启动中的调控作用。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。若需具体论文，建议使用关键词“GTF2E1 recombinant protein”或结合相关研究方向(如结构、突变、功能)进行筛选。
**Background of GTF2E1 Recombinant Protein**
GTF2E1 (General Transcription Factor IIE subunit 1) is a critical component of the RNA polymerase II transcription machinery, playing a pivotal role in the initiation of gene transcription. As part of the TFIIE complex (composed of GTF2E1 and GTF2E2), it facilitates promoter-specific binding of RNA polymerase II and regulates the transition from transcription initiation to elongation. GTF2E1 interacts with other general transcription factors, such as TFIIH, to stabilize the pre-initiation complex and modulate the phosphorylation status of the C-terminal domain (CTD) of RNA polymerase II, a key step in transcriptional regulation.
Recombinant GTF2E1 protein is engineered through molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells), enabling large-scale production for functional and structural studies. Its recombinant form retains biological activity, making it invaluable for *in vitro* assays, including chromatin immunoprecipitation, promoter-binding studies, and investigations into transcription mechanisms. Researchers also utilize it to explore mutations linked to human diseases; for example, GTF2E1 variants are associated with rare developmental disorders, such as trichothiodystrophy, characterized by transcription-coupled DNA repair defects.
Structurally, GTF2E1 contains conserved domains for protein-protein interactions and DNA binding. Recombinant versions often include affinity tags (e.g., His-tag) for purification and detection. Studies using recombinant GTF2E1 have advanced our understanding of transcription dysregulation in cancer, neurodegeneration, and genetic syndromes, highlighting its therapeutic and diagnostic potential. Additionally, it serves as a tool for screening small molecules targeting transcriptional abnormalities.
Overall, GTF2E1 recombinant protein bridges molecular biology and clinical research, offering insights into fundamental transcriptional processes and their pathological disruptions.
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