| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEC |
| Uniprot No | Q15149 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLEC(plectin)重组蛋白的参考文献示例(注：以下内容为模拟概括，实际文献需根据具体研究查询)：
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1. **文献名称**：*"Recombinant Plectin Expression in Epidermolysis Bullosa: Functional Analysis of Mutant Variants"*
**作者**：Smith J, et al.
**摘要**：本研究通过重组技术表达了人源PLEC蛋白的突变体，分析了其在表皮松解症中的致病机制，揭示了plectin在细胞黏附及细胞骨架稳定性中的关键作用。
2. **文献名称**：*"Production and Characterization of Recombinant Plectin for Cytoskeletal Interaction Studies"*
**作者**：Chen L, et al.
**摘要**：报道了一种高效的大肠杆菌重组表达系统，用于制备高纯度PLEC蛋白，并验证其与中间纤维(intermediate filaments)的结合能力，为细胞骨架相互作用研究提供工具。
3. **文献名称**：*"PLEC Isoforms Engineered via Recombinant Technology Reveal Differential Roles in Cell Migration"*
**作者**：Gomez-Ramos P, et al.
**摘要**：通过重组技术构建了PLEC的不同剪接变体，发现特定异构体通过调控肌动蛋白动力学影响癌细胞迁移能力，提示其在肿瘤转移中的潜在靶点价值。
4. **文献名称**：*"Gene Therapy Using Recombinant Plectin for Plectinopathy-associated Muscular Dystrophy"*
**作者**：Tanaka K, et al.
**摘要**：开发了一种基于腺病毒载体的重组PLEC基因递送系统，在动物模型中成功恢复肌肉组织内plectin表达，为遗传性肌病治疗提供新策略。
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建议通过PubMed或Web of Science搜索关键词“recombinant plectin”或“PLEC expression”获取真实文献。
**Background of Recombinant PLEC Protein**
Plectin (PLEC), encoded by the *PLEC* gene, is a giant cytoskeletal protein (~500 kDa) that serves as a critical cytolinker and scaffolding molecule. It plays a pivotal role in maintaining cellular integrity by bridging intermediate filaments, microtubules, and actin filaments, while also anchoring them to membrane-associated complexes like hemidesmosomes and focal adhesions. Plectin’s multidomain structure includes an actin-binding domain, spectrin repeats, and a plakin domain, enabling interactions with diverse binding partners. Mutations in *PLEC* are linked to several human disorders, including epidermolysis bullosa (EB), muscular dystrophy, and cardiomyopathy, underscoring its importance in tissue stability and mechanical stress resistance.
Recombinant PLEC protein refers to plectin produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells) for functional and structural studies. Its large size and complex domain organization pose challenges for recombinant production, often requiring truncated variants or domain-specific constructs. Researchers employ these recombinant proteins to dissect plectin’s roles in cytoskeletal dynamics, organelle positioning, and signal transduction. They are also used to model disease mechanisms, screen therapeutic compounds, and develop protein-replacement strategies for PLEC-related disorders.
Recent advances in protein engineering and cryo-EM have enhanced the study of PLEC’s architecture and interactions. However, limitations persist, such as difficulties in preserving post-translational modifications or full-length protein stability. Despite these hurdles, recombinant PLEC remains indispensable for unraveling its pleiotropic functions and exploring targeted therapies for associated diseases. Ongoing research aims to optimize expression systems and leverage structural insights to design functional mimics or gene-editing solutions, reflecting its broad biomedical relevance.
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