| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBN1 |
| Uniprot No | Q16643 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-649aa |
| 氨基酸序列 | AGVSFSGHR LELLAAYEEV IREESAADWA LYTYEDGSDD LKLAASGEGG LQELSGHFEN QKVMYGFCSV KDSQAALPKY VLINWVGEDV PDARKCACAS HVAKVAEFFQ GVDVIVNASS VEDIDAGAIG QRLSNGLARL SSPVLHRLRL REDENAEPVG TTYQKTDAAV EMKRINREQF WEQAKKEEEL RKEEERKKAL DERLRFEQER MEQERQEQEE RERRYREREQ QIEEHRRKQQ TLEAEEAKRR LKEQSIFGDH RDEEEETHMK KSESEVEEAA AIIAQRPDNP REFFKQQERV ASASAGSCDV PSPFNHRPGS HLDSHRRMAP TPIPTRSPSD SSTASTPVAE QIERALDEVT SSQPPPLPPP PPPAQETQEP SPILDSEETR AAAPQAWAGP MEEPPQAQAP PRGPGSPAED LMFMESAEQA VLAAPVEPAT ADATEIHDAA DTIETDTATA DTTVANNVPP AATSLIDLWP GNGEGASTLQ GEPRAPTPPS GTEVTLAEVP LLDEVAPEPL LPAGEGCATL LNFDELPEPP ATFCDPEEVE GESLAAPQTP TLPSALEELE QEQEPEPHLL TNGETTQKEG TQASEGYFSQ SQEEEFAQSE ELCAKAPPPV FYNKPPEIDI TCWDADPVPE EEEGFEGGD |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DBN1重组蛋白的3篇参考文献示例(内容基于学术文献的典型研究方向概括，具体文献需根据实际检索补充完整信息)：
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1. **文献名称**: "Recombinant DBN1 regulates actin cytoskeleton dynamics in neuronal growth cones"
**作者**: Liu Y, et al.
**摘要**: 本研究成功表达并纯化了人源DBN1重组蛋白，通过体外实验证明其通过结合肌动蛋白微丝调控神经元生长锥的形态变化，揭示了DBN1在神经突触发育中的潜在作用机制。
2. **文献名称**: "Cloning and functional characterization of DBN1 as a tumor suppressor in gastric cancer"
**作者**: Tanaka K, et al.
**摘要**: 文章报道了DBN1重组蛋白的克隆及功能研究，发现其在胃癌细胞中抑制侵袭转移，可能通过稳定细胞骨架结构抑制EMT(上皮间质转化)过程。
3. **文献名称**: "DBN1 recombinant protein interacts with PI3K/AKT signaling pathway in breast cancer cells"
**作者**: Chen X, et al.
**摘要**: 通过体外表达DBN1重组蛋白，研究证实其与PI3K/AKT通路关键分子存在互作，可能通过调控下游信号影响乳腺癌细胞增殖和凋亡。
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**提示**：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词“DBN1 recombinant protein”“DBN1 expression and purification”获取。建议结合具体研究方向(如神经科学、癌症生物学)筛选近年文献。
**Background of DBN1 Recombinant Protein**
DBN1 (Drebrin-like protein 1) is a cytoskeletal protein implicated in regulating actin dynamics, a critical process for cellular structure, motility, and signaling. Originally identified as a member of the drebrin family, DBN1 shares homology with drebrin but exhibits distinct functional roles. It is highly expressed in neuronal tissues, where it participates in synaptic plasticity, dendritic spine formation, and neuronal migration during development. Beyond the nervous system, DBN1 is involved in cell adhesion, cytokinesis, and cancer progression, with studies linking its dysregulation to tumor invasiveness and metastasis.
The recombinant DBN1 protein is engineered using *in vitro* expression systems (e.g., *E. coli* or mammalian cell lines) to produce a purified, bioactive form for experimental applications. This allows researchers to study its interactions with actin filaments, binding partners (e.g., SH3 domain-containing proteins), and regulatory mechanisms in controlled settings. Recombinant DBN1 is widely utilized in structural studies, protein-protein interaction assays, and functional investigations into its role in diseases such as glioblastoma, breast cancer, and neurodevelopmental disorders.
Its modular domains, including an actin-depolymerizing (ADF) domain and a coiled-coil region, make DBN1 a versatile scaffold for cytoskeletal remodeling. Recent studies also highlight its potential as a biomarker or therapeutic target, emphasizing the importance of recombinant DBN1 in both basic research and translational medicine.
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