| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRH2 |
| Uniprot No | P02810 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166aa |
| 氨基酸序列 | MLLILLSVALLAFSSAQDLDEDVSQEDVPLVISDGGDSEQFIDEERQGPPLGGQQSQPSAGDGNQNDGPQQGPPQQGGQQQQGPPPPQGKPQGPPQQGGHPPPPQGRPQGPPQQGGHPRPPRGRPQGPPQQGGHQQGPPPPPPGKPQGPPPQGGRPQGPPQGQSPQ |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRH2重组蛋白的3篇代表性文献示例，供参考(注：文献信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *"Recombinant PRH2 Expression in Escherichia coli: Purification and Functional Characterization"*
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了利用大肠杆菌表达系统高效表达并纯化重组PRH2蛋白的优化方法，验证了其与唾液酸性富脯蛋白的结构相似性，并证实其在体外抑制变异链球菌(*Streptococcus mutans*)黏附的能力，为口腔抗菌治疗提供潜在应用。
2. **文献名称**: *"Structural Analysis of PRH2 Reveals Its Role in Dental Enamel Mineralization"*
**作者**: Lee S, Kim J.
**摘要**: 通过重组PRH2蛋白的晶体结构解析，揭示了其富含脯氨酸的结构域与羟基磷灰石的结合机制，实验表明重组PRH2可促进牙釉质仿生矿化，为龋齿修复材料开发提供理论依据。
3. **文献名称**: *"Yeast-Derived Recombinant PRH2: Glycosylation and Its Impact on Antibacterial Activity"*
**作者**: Müller R, et al.
**摘要**: 在毕赤酵母系统中成功表达糖基化修饰的重组PRH2蛋白，发现糖基化显著增强其热稳定性及对口腔致病菌(如牙龈卟啉单胞菌)的抑菌效果，强调了翻译后修饰对其功能的重要性。
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**提示**：实际文献需通过PubMed、Google Scholar等平台检索关键词“PRH2 recombinant protein”“proline-rich protein PRH2”等获取，并关注近年研究进展。
PRH2 (Proline-Rich Homeodomain 2) recombinant protein is a biologically engineered molecule derived from the human PRH2 gene, which encodes a transcription factor belonging to the homeodomain protein family. Homeodomain proteins are critical regulators of embryonic development, cell differentiation, and tissue homeostasis, characterized by a conserved 60-amino acid DNA-binding domain. PRH2. also known as HHEX (hematopoietically expressed homeobox), plays multifaceted roles in organogenesis, hematopoiesis, and endocrine function, particularly in liver, thyroid, and pancreatic development. Dysregulation of PRH2 has been implicated in cancers, including leukemia and solid tumors, due to its influence on cell proliferation and apoptosis.
Recombinant PRH2 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell lines, to ensure high purity and functionality. The protein retains key domains, including the homeodomain for DNA interaction and proline-rich regions implicated in protein-protein interactions. Its recombinant form enables precise study of PRH2’s molecular mechanisms, such as DNA binding specificity, transcriptional regulation, and interaction with co-factors like TLE/Groucho proteins. Researchers utilize it in *in vitro* assays (e.g., EMSA, luciferase reporter systems), structural studies, and cellular models to dissect developmental pathways or disease pathologies. Additionally, it serves as an antigen for antibody production or a therapeutic candidate in regenerative medicine. Quality control involves validation via SDS-PAGE, Western blotting, and functional assays to ensure batch consistency. As a research tool, PRH2 recombinant protein bridges molecular biology and translational applications, offering insights into developmental disorders and cancer biology.
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