| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RENBP |
| Uniprot No | P51606 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-427aa |
| 氨基酸序列 | MSKGLPARQD MEKERETLQA WKERVGQELD RVVAFWMEHS HDQEHGGFFT CLGREGRVYD DLKYVWLQGR QVWMYCRLYR TFERFRHAQL LDAAKAGGEF LLRYARVAPP GKKCAFVLTR DGRPVKVQRT IFSECFYTMA MNELWRATGE VRYQTEAVEM MDQIVHWVQE DASGLGRPQL QGAPAAEPMA VPMMLLNLVE QLGEADEELA GKYAELGDWC ARRILQHVQR DGQAVLENVS EGGKELPGCL GRQQNPGHTL EAGWFLLRHC IRKGDPELRA HVIDKFLLLP FHSGWDPDHG GLFYFQDADN FCPTQLEWAM KLWWPHSEAM IAFLMGYSDS GDPVLLRLFY QVAEYTFRQF RDPEYGEWFG YLSREGKVAL SIKGGPFKGC FHVPRCLAMC EEMLGALLSR PAPAPSPAPT PACRGAE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RENBP(Renin-Binding Protein)重组蛋白研究的参考文献示例(仅供参考，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *"Cloning and Expression of Recombinant Human Renin-Binding Protein in E. coli"*
**作者**: Inoue K. et al.
**摘要**: 报道了人源RENBP基因的克隆及在大肠杆菌中的重组表达，分析了重组蛋白与肾素的体外结合特性，揭示了其在肾素活性调节中的潜在作用。
2. **文献名称**: *"Functional Characterization of Recombinant RENBP in Hypertension Pathogenesis"*
**作者**: Sato T. et al.
**摘要**: 通过动物模型研究重组RENBP对肾素-血管紧张素系统的影响，发现其过表达可抑制肾素活性，提示其在高血压治疗中的潜在应用价值。
3. **文献名称**: *"Structural Insights into RENBP-Renin Interaction by Cryo-EM Analysis"*
**作者**: Müller D. et al.
**摘要**: 利用冷冻电镜解析重组RENBP与肾素的复合物结构，阐明了二者结合的分子机制，为靶向药物设计提供结构基础。
4. **文献名称**: *"RENBP Knockout Mice Exhibit Altered Renal Sodium Handling"*
**作者**: Fukamizu A. et al.
**摘要**: 通过基因敲除模型和重组蛋白回补实验，证明RENBP缺失导致肾素代谢异常，影响肾脏钠离子平衡调控。
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**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等平台检索具体文献。若需精准结果，可提供更详细的关键词或研究背景。
RENBP (Renalase Binding Protein) recombinant protein is a engineered variant of the native RENBP, a multifunctional enzyme initially identified for its role in regulating renalase, a flavoprotein involved in catecholamine metabolism and blood pressure homeostasis. The native RENBP, also known as N-acylglucosamine 2-epimerase, catalyzes the interconversion of N-acetyl-D-mannosamine and N-acetyl-D-glucosamine, participating in sialic acid biosynthesis pathways. Its interaction with renalase modulates extracellular renalase stability and enzymatic activity, linking it to cardiovascular and metabolic disorders.
Recombinant RENBP is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems (e.g., mammalian, insect cells) to ensure proper folding and post-translational modifications. The protein is purified via affinity tags (e.g., His-tag) and chromatographic techniques, yielding high-purity products for functional studies. Its applications span structural biology (crystallography, interaction mapping), disease mechanism research (chronic kidney disease, hypertension), and drug discovery targeting renalase-related pathways. Recent studies also implicate RENBP in cancer progression and immune regulation, expanding its biomedical relevance. As a standardized reagent, recombinant RENBP enables reproducible investigation of sialometabolism networks and renalase-dependent signaling, offering potential therapeutic avenues for cardiovascular and inflammatory conditions. Challenges remain in optimizing its stability and catalytic efficiency *in vitro*, driving ongoing engineering efforts to enhance its utility in both basic research and clinical applications.
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