| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GCNF |
| Uniprot No | Q15406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-480aa |
| 氨基酸序列 | MERDEPPPSG GGGGGGSAGF LEPPAALPPP PRNGFCQDEL AELDPGTISV SDDRAEQRTC LICGDRATGL HYGIISCEGC KGFFKRSICN KRVYRCSRDK NCVMSRKQRN RCQYCRLLKC LQMGMNRKAI REDGMPGGRN KSIGPVQISE EEIERIMSGQ EFEEEANHWS NHGDSDHSSP GNRASESNQP SPGSTLSSSR SVELNGFMAF REQYMGMSVP PHYQYIPHLF SYSGHSPLLP QQARSLDPQS YSLIHQLLSA EDLEPLGTPM LIEDGYAVTQ AELFALLCRL ADELLFRQIA WIKKLPFFCE LSIKDYTCLL SSTWQELILL SSLTVYSKQI FGELADVTAK YSPSDEELHR FSDEGMEVIE RLIYLYHKFH QLKVSNEEYA CMKAINFLNQ DIRGLTSASQ LEQLNKRYWY ICQDFTEYKY THQPNRFPDL MMCLPEIRYI AGKMVNVPLE QLPLLFKVVL HSCKTSVGKE |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GCNF(Germ Cell Nuclear Factor)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: "The orphan nuclear receptor GCNF recruits DNA methyltransferase for transcriptional repression in mouse embryonic stem cells"
**作者**: Gu P, et al.
**摘要**: 研究揭示了重组GCNF蛋白在小鼠胚胎干细胞中通过招募DNA甲基转移酶抑制靶基因转录的分子机制，表明GCNF在表观遗传调控和干细胞分化中起关键作用。
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2. **文献名称**: "Structural and functional analysis of germ cell nuclear factor (GCNF) as a transcriptional repressor"
**作者**: Cooney AJ, et al.
**摘要**: 通过重组GCNF蛋白的体外表达与结构分析，阐明了其DNA结合域和配体结合域的结构特征，并证明GCNF通过结合特定DNA序列抑制生殖细胞分化相关基因的表达。
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3. **文献名称**: "GCNF-dependent regulation of pluripotency and differentiation in early embryos"
**作者**: Susens U, et al.
**摘要**: 利用重组GCNF蛋白进行功能实验，发现其在早期胚胎发育中通过调控Oct4等多能性因子的表达，参与胚胎干细胞多能性维持与向生殖细胞谱系分化的转换。
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(注：以上文献信息为示例性概括，实际文献需通过学术数据库检索确认。)
**Background of GCNF Recombinant Protein**
GCNF (Germ Cell Nuclear Factor), also known as NR6A1. is a member of the nuclear receptor superfamily, which plays critical roles in transcriptional regulation. Initially identified for its expression in germ cells, GCNF is a ligand-binding transcription factor characterized by a conserved DNA-binding domain (DBD) and a ligand-binding domain (LBD). Unlike many nuclear receptors, GCNF lacks a classical activation function and primarily acts as a transcriptional repressor. It regulates gene expression by binding to specific DNA response elements, often repressing target genes involved in embryonic development, germ cell differentiation, and metabolic processes.
GCNF is essential during early embryogenesis, particularly in regulating the transition from pluripotency to differentiation in embryonic stem cells. Studies in knockout mice have highlighted its role in neural development, organogenesis, and germ cell maturation, with GCNF deficiency leading to embryonic lethality or severe developmental defects. Its involvement in silencing pluripotency genes, such as *Oct4* and *Nanog*, underscores its importance in maintaining developmental timelines.
Recombinant GCNF protein is produced using expression systems like *E. coli* or mammalian cells, enabling biochemical and functional studies. Purified GCNF is utilized to investigate its DNA-binding properties, interaction with co-repressors, and regulatory mechanisms in vitro. As an orphan receptor (endogenous ligand unknown), structural studies of recombinant GCNF help elucidate its ligand-binding pocket and conformational dynamics, offering insights into potential therapeutic targeting.
Research on GCNF recombinant protein also extends to disease models, including cancer and neurodegenerative disorders, where dysregulation of GCNF-mediated pathways is implicated. Its role in metabolic regulation further links it to conditions like diabetes and obesity. Overall, GCNF remains a pivotal molecule for understanding developmental biology and designing interventions for related pathologies.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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