| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COA4 |
| Uniprot No | Q9NYJ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-87aa |
| 氨基酸序列 | MSTSVPQGHT WTQRVKKDDE EEDPLDQLIS RSGCAASHFA VQECMAQHQD WRQCQPQVQA FKDCMSEQQA RRQEELQRRQ EQAGAHH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于COA4重组蛋白的模拟参考文献(内容为虚构示例，仅供参考)：
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1. **文献名称**: "Recombinant COA4 Protein Facilitates Cytochrome c Oxidase Assembly in Mitochondrial Disorders"
**作者**: Zhang L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组COA4蛋白，验证了其与线粒体复合体IV亚基的相互作用。实验表明，重组COA4可恢复模型细胞中复合体IV的组装缺陷，为相关线粒体疾病的治疗提供了潜在策略。
2. **文献名称**: "Structural and Functional Characterization of Human COA4 in vitro"
**作者**: Müller S, et al.
**摘要**: 通过昆虫细胞系统表达重组人源COA4蛋白，结合X射线晶体学解析其三维结构，揭示了COA4中关键的金属结合域。功能实验表明，重组COA4通过螯合铜离子参与复合体IV的成熟过程。
3. **文献名称**: "COA4 Knockdown and Rescue via Recombinant Protein in Zebrafish Models"
**作者**: Chen X, et al.
**摘要**: 利用斑马鱼模型研究COA4功能，发现其缺失导致心脏发育异常。通过显微注射重组COA4蛋白可部分挽救表型，证实其在胚胎发育中对线粒体能量代谢的调控作用。
4. **文献名称**: "High-Yield Production of COA4 in HEK293 Cells for Drug Screening Applications"
**作者**: Gupta R, et al.
**摘要**: 开发了一种基于HEK293细胞的重组COA4高效表达系统，优化纯化流程后获得高纯度蛋白。该蛋白被用于筛选靶向复合体IV组装的小分子化合物，发现多个潜在先导分子。
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注：上述文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
**Background of COA4 Recombinant Protein**
COA4 (Cytochrome c Oxidase Assembly Factor 4), also known as MITRAC7. is a mitochondrial protein critical for the biogenesis of cytochrome c oxidase (COX), the terminal enzyme in the mitochondrial electron transport chain (ETC). COX catalyzes the transfer of electrons to oxygen during oxidative phosphorylation, a process essential for cellular energy (ATP) production. COA4 functions as a chaperone-like assembly factor, stabilizing newly synthesized COX subunits (e.g., COX1) and ensuring proper incorporation into the holoenzyme. It interacts with the MITRAC (mitochondrial translation regulation assembly intermediate of COX) complex, coordinating COX assembly and quality control.
Mutations or dysregulation of COX assembly factors, including COA4. are linked to mitochondrial disorders and diseases such as Leigh syndrome, neurodegenerative conditions, and cancer. Recombinant COA4 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional roles. Its recombinant form enables *in vitro* analyses, including protein-protein interaction assays, structural studies, and screening for therapeutic agents targeting mitochondrial dysfunction. Research on COA4 also contributes to understanding cellular responses to oxidative stress and metabolic reprogramming in pathological states. Overall, COA4 recombinant protein serves as a vital tool for dissecting mitochondrial COX assembly mechanisms and exploring therapeutic strategies for energy metabolism-related diseases.
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