| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEFL |
| Uniprot No | P07196 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-543aa |
| 氨基酸序列 | SSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSL SVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRF ASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAE DATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADE AALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTK PDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAA KDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTI NKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLS FTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQ IEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEE SEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD |
| 预测分子量 | 81 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NEFL(神经丝轻链蛋白)重组蛋白研究的3篇参考文献示例(注：文献信息为示例性概括，非真实引用)：
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1. **文献名称**：*Expression and purification of recombinant human neurofilament light chain in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：该研究建立了人源NEFL蛋白在大肠杆菌中的重组表达系统，优化了诱导条件和纯化步骤(如His标签亲和层析)，获得了高纯度蛋白，为后续抗体开发及体外神经退行性疾病模型研究提供材料基础。
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2. **文献名称**：*Structural analysis of NEFL aggregates in amyotrophic lateral sclerosis using recombinant protein models*
**作者**：Chen L, et al.
**摘要**：通过重组表达NEFL蛋白，结合冷冻电镜技术解析了其在肌萎缩侧索硬化症(ALS)患者中异常聚集的分子机制，揭示了磷酸化修饰对蛋白聚集的促进作用，为靶向治疗提供结构依据。
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3. **文献名称**：*NEFL as a biomarker in cerebrospinal fluid: validation of a recombinant protein-based ELISA assay*
**作者**：Wang Y, et al.
**摘要**：研究开发了一种基于重组NEFL蛋白的ELISA检测方法，用于定量脑脊液中的NEFL水平，验证其在阿尔茨海默病和帕金森病中的诊断价值，证明其与神经元损伤程度呈正相关。
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**提示**：实际文献检索可使用PubMed或Google Scholar，以关键词“recombinant NEFL protein”、“neurofilament light chain expression”等进行查询，并筛选涉及重组表达技术、病理机制或生物标志物应用的研究。
**Background of NEFL Recombinant Protein**
Neurofilament light polypeptide (NEFL), a key component of neuronal intermediate filaments, is critical for maintaining the structural integrity and diameter of axons in both the central and peripheral nervous systems. Belonging to the neurofilament triplet protein family alongside neurofilament medium (NEFM) and heavy (NEFH) chains, NEFL forms the core scaffold of neuronal cytoskeletons. These filaments regulate axonal transport, mechanical stability, and signal transduction, with NEFL’s smaller size enabling dynamic assembly and disassembly.
Dysregulation of NEFL expression or mutations in the *NEFL* gene is linked to neurodegenerative disorders such as Charcot-Marie-Tooth disease, amyotrophic lateral sclerosis (ALS), and Parkinson’s disease. Elevated levels of NEFL in cerebrospinal fluid (CSF) and blood are recognized biomarkers for axonal damage, aiding in early diagnosis and disease progression monitoring.
Recombinant NEFL protein, produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), ensures high purity and consistency for research and clinical applications. Its production involves cloning the *NEFL* gene into expression vectors, followed by purification using affinity chromatography. This recombinant tool facilitates studies on neurofilament dynamics, antibody development for diagnostic assays, and drug screening targeting neuroprotection.
By enabling precise investigation of neurodegeneration mechanisms, NEFL recombinant protein serves as a vital resource in both basic neuroscience and therapeutic development.
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