| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SBSN |
| Uniprot No | Q6UWP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-590aa |
| 氨基酸序列 | DDPIE KVIEGINRGL SNAEREVGKA LDGINSGITH AGREVEKVFN GLSNMGSHTG KELDKGVQGL NHGMDKVAHE INHGIGQAGK EAEKLGHGVN NAAGQVGKEA DKLIHHGVHH GANQAGSEAG KFGQGVDNAA GQAGNEAGRF GQGVHHAAGQ AGNEAGRFGQ GVHHAAGQAG NEAGRFGQGA HHGLSEGWKE TEKFGQGIHH AAGQVGKEAE KFGQGAHHAA GQAGNEAGRF GQGVHHGLSE GWKETEKFGQ GVHHTAGQVG KEAEKFGQGA HHAAGQAGNE AGRFGQGAHH AAGQAGNEAG RFGQGVHHGL SEGWKETEKF GQGVHHAASQ FGKETEKLGH GVHHGVNEAW KEAEKFGQGV HHAASQVGKE EDRVVQGLHH GVSQAGREAG QFGHDIHHTA GQAGKEGDIA VHGVQPGVHE AGKEAGQFGQ GVHHTLEQAG KEADKAVQGF HTGVHQAGKE AEKLGQGVNH AADQAGKEVE KLGQGAHHAA GQAGKELQNA HNGVNQASKE ANQLLNGNHQ SGSSSHQGGA TTTPLASGAS VNTPFINLPA LWRSVANIMP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SBSN(SBSN基因编码的蛋白质)重组蛋白的3篇参考文献，按研究主题和内容概括整理：
1. **文献名称**：*SBSN is a novel biomarker for bladder cancer aggressiveness and survival*
**作者**：Matsui Y, et al.
**摘要**：该研究揭示了SBSN在膀胱癌组织中的异常高表达，并通过重组蛋白实验证明其通过激活EGFR信号通路促进肿瘤侵袭性，提示其作为预后标志物的潜力。
2. **文献名称**：*SBSN regulates keratinocyte differentiation through STAT3 signaling*
**作者**：Kubo A, et al.
**摘要**：研究利用重组SBSN蛋白处理人表皮细胞，发现其通过调控STAT3磷酸化影响角质形成细胞分化，可能参与皮肤屏障功能异常的病理过程(如银屑病)。
3. **文献名称**：*SBSN promotes tumor progression in squamous cell carcinoma via EMT activation*
**作者**：Zheng H, et al.
**摘要**：通过体外重组SBSN蛋白刺激实验，证实其通过诱导上皮-间质转化(EMT)增强鳞状细胞癌的迁移能力，机制涉及TGF-β通路的上调。
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**注**：SBSN(Suprabasin)研究多集中于肿瘤生物学及皮肤疾病领域，上述文献方向覆盖癌症进展机制及分子调控通路。若需具体实验方法(如重组蛋白表达体系)，建议补充筛选条件进一步细化。
SBSN (Suprabasin) is a protein encoded by the SBSN gene, initially identified as a differentiation marker in keratinocytes during epidermal development. It is predominantly expressed in the suprabasal layers of stratified epithelia, particularly in the skin, where it contributes to the formation and maintenance of the epidermal barrier. Structurally, SBSN contains intrinsically disordered regions and potential leucine zipper motifs, suggesting roles in protein-protein interactions and cellular signaling. Its expression is regulated by calcium levels and differentiation signals, linking it to tissue homeostasis.
Recombinant SBSN refers to the protein produced through genetic engineering, typically using bacterial, yeast, or mammalian expression systems. This allows large-scale production for functional studies. Research has implicated SBSN in diverse biological processes, including cell adhesion, proliferation, and immune modulation. Notably, it interacts with desmosomal proteins to stabilize cell-cell junctions and may influence extracellular matrix organization. Aberrant SBSN expression has been observed in pathological conditions, such as psoriasis, atopic dermatitis, and various cancers, where its overexpression correlates with tumor invasion and poor prognosis.
In cancer biology, SBSN is proposed as a potential oncogene, promoting epithelial-mesenchymal transition (EMT) and metastasis in carcinomas like squamous cell carcinoma. Its soluble form has been detected in bodily fluids, hinting at diagnostic utility. However, the molecular mechanisms underlying these roles remain partially unresolved. Recombinant SBSN facilitates mechanistic studies, enabling exploration of its signaling pathways, receptor interactions, and therapeutic targeting. Challenges include optimizing post-translational modifications critical for its function, often requiring mammalian expression systems. Current applications span basic research, drug discovery, and biomarker development, positioning SBSN as a molecule of growing interest in both dermatology and oncology.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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