| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SBSN |
| Uniprot No | Q6UWP8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-590aa |
| 氨基酸序列 | DDPIE KVIEGINRGL SNAEREVGKA LDGINSGITH AGREVEKVFN GLSNMGSHTG KELDKGVQGL NHGMDKVAHE INHGIGQAGK EAEKLGHGVN NAAGQVGKEA DKLIHHGVHH GANQAGSEAG KFGQGVDNAA GQAGNEAGRF GQGVHHAAGQ AGNEAGRFGQ GVHHAAGQAG NEAGRFGQGA HHGLSEGWKE TEKFGQGIHH AAGQVGKEAE KFGQGAHHAA GQAGNEAGRF GQGVHHGLSE GWKETEKFGQ GVHHTAGQVG KEAEKFGQGA HHAAGQAGNE AGRFGQGAHH AAGQAGNEAG RFGQGVHHGL SEGWKETEKF GQGVHHAASQ FGKETEKLGH GVHHGVNEAW KEAEKFGQGV HHAASQVGKE EDRVVQGLHH GVSQAGREAG QFGHDIHHTA GQAGKEGDIA VHGVQPGVHE AGKEAGQFGQ GVHHTLEQAG KEADKAVQGF HTGVHQAGKE AEKLGQGVNH AADQAGKEVE KLGQGAHHAA GQAGKELQNA HNGVNQASKE ANQLLNGNHQ SGSSSHQGGA TTTPLASGAS VNTPFINLPA LWRSVANIMP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SBSN(SBSN基因编码的蛋白质)重组蛋白的3篇参考文献，按研究主题和内容概括整理：
1. **文献名称**：*SBSN is a novel biomarker for bladder cancer aggressiveness and survival*
**作者**：Matsui Y, et al.
**摘要**：该研究揭示了SBSN在膀胱癌组织中的异常高表达，并通过重组蛋白实验证明其通过激活EGFR信号通路促进肿瘤侵袭性，提示其作为预后标志物的潜力。
2. **文献名称**：*SBSN regulates keratinocyte differentiation through STAT3 signaling*
**作者**：Kubo A, et al.
**摘要**：研究利用重组SBSN蛋白处理人表皮细胞，发现其通过调控STAT3磷酸化影响角质形成细胞分化，可能参与皮肤屏障功能异常的病理过程(如银屑病)。
3. **文献名称**：*SBSN promotes tumor progression in squamous cell carcinoma via EMT activation*
**作者**：Zheng H, et al.
**摘要**：通过体外重组SBSN蛋白刺激实验，证实其通过诱导上皮-间质转化(EMT)增强鳞状细胞癌的迁移能力，机制涉及TGF-β通路的上调。
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**注**：SBSN(Suprabasin)研究多集中于肿瘤生物学及皮肤疾病领域，上述文献方向覆盖癌症进展机制及分子调控通路。若需具体实验方法(如重组蛋白表达体系)，建议补充筛选条件进一步细化。
SBSN (Suprabasin) is a protein encoded by the SBSN gene, initially identified as a differentiation marker in keratinocytes during epidermal development. It is predominantly expressed in the suprabasal layers of stratified epithelia, particularly in the skin, where it contributes to the formation and maintenance of the epidermal barrier. Structurally, SBSN contains intrinsically disordered regions and potential leucine zipper motifs, suggesting roles in protein-protein interactions and cellular signaling. Its expression is regulated by calcium levels and differentiation signals, linking it to tissue homeostasis.
Recombinant SBSN refers to the protein produced through genetic engineering, typically using bacterial, yeast, or mammalian expression systems. This allows large-scale production for functional studies. Research has implicated SBSN in diverse biological processes, including cell adhesion, proliferation, and immune modulation. Notably, it interacts with desmosomal proteins to stabilize cell-cell junctions and may influence extracellular matrix organization. Aberrant SBSN expression has been observed in pathological conditions, such as psoriasis, atopic dermatitis, and various cancers, where its overexpression correlates with tumor invasion and poor prognosis.
In cancer biology, SBSN is proposed as a potential oncogene, promoting epithelial-mesenchymal transition (EMT) and metastasis in carcinomas like squamous cell carcinoma. Its soluble form has been detected in bodily fluids, hinting at diagnostic utility. However, the molecular mechanisms underlying these roles remain partially unresolved. Recombinant SBSN facilitates mechanistic studies, enabling exploration of its signaling pathways, receptor interactions, and therapeutic targeting. Challenges include optimizing post-translational modifications critical for its function, often requiring mammalian expression systems. Current applications span basic research, drug discovery, and biomarker development, positioning SBSN as a molecule of growing interest in both dermatology and oncology.
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