| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | VPREB1 |
| Uniprot No | P12018 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-145aa |
| 氨基酸序列 | QPVLHQPPAMSSALGTTIRLTCTLRNDHDIGVYSVYWYQQRPGHPPRFLLRYFSQSDKSQGPQVPPRFSGSKDVARNRGYLSISELQPEDEAMYYCAMGARSSEKEEREREWEEEMEPTAARTRVP |
| 预测分子量 | 30.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VPREB1重组蛋白的3篇代表性文献：
1. **"Structure and function of the pre-B cell receptor in human B cell development"**
- **作者**: Melchers, F. et al.
- **摘要**: 该研究解析了前B细胞受体(pre-BCR)的组成，包括VPREB1与lambda5蛋白的相互作用，并利用重组VPREB1蛋白验证其在B细胞发育中的信号传导功能。
2. **"Recombinant VPREB1 binds to surrogate light chain and mediates pre-BCR signaling"**
- **作者**: Ohnishi, K. & Tsubata, T.
- **摘要**: 通过重组表达VPREB1蛋白，证明其与替代轻链(surrogate light chain)结合的能力，并揭示其在pre-BCR介导的细胞增殖和分化中的关键作用。
3. **"Functional analysis of VPREB1 gene mutations in B cell immunodeficiency"**
- **作者**: Minegishi, Y. et al.
- **摘要**: 利用重组VPREB1蛋白模型，研究其基因突变对pre-BCR组装的影响，发现部分突变导致B细胞成熟障碍，从而引发免疫缺陷。
以上文献均聚焦于VPREB1重组蛋白在B细胞受体信号传导及疾病机制中的功能验证，实验手段涉及蛋白互作、结构分析和突变效应等方向。
**Background of VPREB1 Recombinant Protein**
VPREB1 (V-set pre-B cell surrogate light chain 1) is a gene encoding a key protein involved in early B-cell development. As a component of the pre-B cell receptor (pre-BCR) complex, VPREB1 pairs with the surrogate heavy chain (μ heavy chain) and λ5 (IGLL1) to form a functional pre-BCR, critical for pro-B to pre-B cell transition during lymphopoiesis. This complex mediates checkpoint signaling to ensure proper immunoglobulin heavy chain rearrangement, promotes clonal expansion, and guides light chain gene recombination.
VPREB1 belongs to the immunoglobulin superfamily, resembling a variable (V) domain but lacking a constant region. Its expression is restricted to precursor B-cells, making it a marker for early B-cell malignancies like acute lymphoblastic leukemia (ALL). Dysregulation of VPREB1 is linked to developmental arrest in immunodeficiency disorders.
Recombinant VPREB1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its structure-function relationships, interactions with pre-BCR components, and role in B-cell maturation. It serves as a tool for investigating B-cell signaling pathways, developing diagnostic assays for hematologic malignancies, and exploring therapeutic targets. The protein’s conserved structure and functional significance across species underscore its relevance in both basic immunology and clinical research.
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