| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDE10A |
| Uniprot No | Q9Y233 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 71-779aa |
| 氨基酸序列 | MQGVVYELNSYIEQRLDTGGDNQLLLYELSSIIKIATKADGFALYFLGEC NNSLCIFTPPGIKEGKPRLIPAGPITQGTTVSAYVAKSRKTLLVEDILGD ERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFCLSHQ EVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSL LEHIMIYAKNLVNADRCALFQVDHKNKELYSDLFDIGEEKEGKPVFKKTK EIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREVDLYTGYTTRNILCM PIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHR IRHSECIYRVTMEKLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIG PFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHA VTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDH PLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAI IATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTK LWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFY NAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSV AQKAAASED |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PDE10A重组蛋白的参考文献及其摘要概括：
1. **"Structural basis for the activity of drugs that inhibit phosphodiesterases"**
*Chappie, T.A., et al. (2009)*
该研究通过X射线晶体学解析了重组人源PDE10A的催化结构域与抑制剂的复合物结构，阐明了其选择性抑制机制，并详细描述了使用昆虫细胞表达系统进行重组蛋白表达与纯化的方法。
2. **"Expression and characterization of the human recombinant PDE10A: a comparative study of PDE10A variants"**
*Kotera, J., et al. (2004)*
作者在大肠杆菌和哺乳动物细胞中重组表达了人源PDE10A的两种剪接变体，分析了其酶动力学特性及对抑制剂的敏感性，为靶向药物开发提供了功能数据。
3. **"Development of a high-throughput screening assay for PDE10A using recombinant enzyme"**
*Jensen, J.B., et al. (2011)*
研究报道了一种基于重组PDE10A蛋白的高通量筛选平台，利用荧光底物检测酶活性，成功应用于大规模药物候选分子库的筛选与优化。
**Background of PDE10A Recombinant Protein**
Phosphodiesterase 10A (PDE10A) is a member of the phosphodiesterase enzyme family responsible for hydrolyzing cyclic nucleotides (cAMP and cGMP), critical secondary messengers in cellular signaling. Primarily expressed in the brain, particularly in the striatum, PDE10A regulates neurotransmission pathways linked to motor control, cognition, and emotion. Its unique tissue distribution and functional role have made it a target for studying neuropsychiatric and neurodegenerative disorders, such as Huntington’s disease, schizophrenia, and Parkinson’s disease.
Recombinant PDE10A protein is produced via genetic engineering, enabling large-scale in vitro expression for research and therapeutic development. The protein typically retains its catalytic domain and regulatory regions, allowing studies on enzyme kinetics, inhibitor screening, and structural analysis. Researchers often use bacterial, insect, or mammalian expression systems to generate active, purified PDE10A, sometimes incorporating tags (e.g., His-tag) for simplified isolation.
Structurally, PDE10A contains two N-terminal GAF domains (involved in dimerization and regulatory interactions) and a C-terminal catalytic domain. Its activity is modulated by phosphorylation and substrate availability, influencing downstream signaling cascades. Dysregulation of PDE10A is implicated in pathological conditions; for example, reduced PDE10A levels correlate with striatal degeneration in Huntington’s disease. Conversely, PDE10A inhibitors are explored as potential therapeutics to modulate cAMP/cGMP balance in psychiatric disorders.
Recombinant PDE10A is vital for drug discovery, enabling high-throughput screening of inhibitors and structure-based drug design. It also aids in elucidating molecular mechanisms of PDE10A-related diseases and validating biomarkers. Studies using recombinant protein have advanced understanding of PDE10A’s role in synaptic plasticity, dopamine signaling, and neuronal survival, highlighting its therapeutic and diagnostic potential in neuroscience.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
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艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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