| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PICALM |
| Uniprot No | Q13492 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-652aa |
| 氨基酸序列 | SGQSLTDRI TAAQHSVTGS AVSKTVCKAT THEIMGPKKK HLDYLIQCTN EMNVNIPQLA DSLFERTTNS SWVVVFKSLI TTHHLMVYGN ERFIQYLASR NTLFNLSNFL DKSGLQGYDM STFIRRYSRY LNEKAVSYRQ VAFDFTKVKR GADGVMRTMN TEKLLKTVPI IQNQMDALLD FNVNSNELTN GVINAAFMLL FKDAIRLFAA YNEGIINLLE KYFDMKKNQC KEGLDIYKKF LTRMTRISEF LKVAEQVGID RGDIPDLSQA PSSLLDALEQ HLASLEGKKI KDSTAASRAT TLSNAVSSLA STGLSLTKVD EREKQAALEE EQARLKALKE QRLKELAKKP HTSLTTAASP VSTSAGGIMT APAIDIFSTP SSSNSTSKLP NDLLDLQQPT FHPSVHPMST ASQVASTWGD PFSATVDAVD DAIPSLNPFL TKSSGDVHLS ISSDVSTFTT RTPTHEMFVG FTPSPVAQPH PSAGLNVDFE SVFGNKSTNV IVDSGGFDEL GGLLKPTVAS QNQNLPVAKL PPSKLVSDDL DSSLANLVGN LGIGNGTTKN DVNWSQPGEK KLTGGSNWQP KVAPTTAWNA ATMAPPVMAY PATTPTGMIG YGIPPQMGSV PVMTQPTLIY SQPVMRPPNP FGPVSGAQIQ FM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PICALM重组蛋白的3篇模拟参考文献示例(注：以下为假设性文献，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*Structural and functional characterization of the PICALM ANTH domain in phosphoinositide recognition*
**作者**：Miller, S.E., et al.
**摘要**：通过重组表达PICALM的ANTH结构域，结合X射线晶体学分析，揭示了其与磷脂酰肌醇(PI)相互作用的分子机制，阐明其在细胞内吞中的脂质结合特异性。
2. **文献名称**：*PICALM modulates tau aggregation and clearance in Alzheimer’s disease models*
**作者**：Honda, T., et al.
**摘要**：利用重组PICALM蛋白研究其与tau蛋白的相互作用，发现PICALM通过促进自噬途径清除异常tau聚集体，为阿尔茨海默病的治疗提供潜在靶点。
3. **文献名称**：*Recombinant PICALM fusion proteins as tools to study clathrin-mediated endocytosis*
**作者**：Dergai, O., et al.
**摘要**：开发了带有荧光标签的重组PICALM蛋白，通过体外膜结合实验证明其与网格蛋白、AP2复合物的协同作用，揭示了其在膜变形过程中的动态调控机制。
如需真实文献，建议通过PubMed或Google Scholar检索关键词“PICALM recombinant protein”、“PICALM purification”或“PICALM structure function”。
PICALM (Phosphatidylinositol Binding Clathrin Assembly Protein), also known as CALM, is a ubiquitously expressed protein involved in clathrin-mediated endocytosis (CME), intracellular trafficking, and synaptic vesicle recycling. It functions as an adaptor protein, facilitating the assembly of clathrin-coated vesicles by linking clathrin to phosphatidylinositol-enriched membranes. Structurally, PICALM contains an N-terminal ANTH (AP180 N-terminal homology) domain that binds phosphoinositides, a central clathrin-binding motif, and a C-terminal region mediating interactions with other endocytic components like AP2 and Epsin1. These features enable its role in membrane curvature induction and cargo selection during vesicle formation.
PICALM’s biological significance extends to multiple physiological and pathological processes. Genetic studies have linked PICALM mutations to hematopoietic disorders, particularly acute myeloid leukemia (AML), where chromosomal translocations (e.g., t(10;11)) generate fusion oncoproteins. Additionally, genome-wide association studies (GWAS) implicate PICALM as a susceptibility gene in Alzheimer’s disease (AD), likely through its influence on amyloid precursor protein (APP) processing and clearance via endocytic pathways. Dysregulation of PICALM is also associated with autophagy defects and neurodegeneration.
Recombinant PICALM proteins, produced in systems like *E. coli* or mammalian cells, are essential tools for studying these mechanisms. They enable *in vitro* analysis of protein-protein interactions, membrane binding kinetics, and structural features via techniques like pull-down assays, liposome binding experiments, or crystallography. Such studies help elucidate how PICALM variants alter cellular trafficking or contribute to disease pathogenesis. Furthermore, recombinant PICALM serves as an antigen for antibody development or a functional reagent in drug screening platforms targeting endocytosis-related disorders. Its versatile applications underscore its importance in both basic research and therapeutic exploration.
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