| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNPEP |
| Uniprot No | Q9H4A4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-650aa |
| 氨基酸序列 | MASGEHSPGSGAARRPLHSAQAVDVASASNFRAFELLHLHLDLRAEFGPP GPGAGSRGLSGTAVLDLRCLEPEGAAELRLDSHPCLEVTAAALRRERPGS EEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLLTYRVGEGPGV CWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDG FTAVMSASTWEKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVW AEPCLIDAAKEEYNGVIEEFLATGEKLFGPYVWGRYDLLFMPPSFPFGGM ENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGF TMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIE PGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSI LADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPPYLPDLSPGDS LMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPP GNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGK QKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQIVAPKGS |
| 预测分子量 | 99 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNPEP重组蛋白的3篇参考文献示例(内容基于学术文献的常见研究方向模拟整理)：
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1. **文献名称**：*"Cloning and Expression of Recombinant Human RNPEP in E. coli: Enzymatic Characterization"*
**作者**：Chen L, et al.
**摘要**：本研究成功克隆了人源RNPEP基因，并在大肠杆菌中实现了高效可溶性表达。通过纯化获得的重组蛋白具有精氨酸氨基肽酶活性，体外实验证实其对血管紧张素II的水解作用，为研究其在血压调节中的功能提供了工具。
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2. **文献名称**：*"Structural Insights into RNPEP Substrate Specificity by X-ray Crystallography"*
**作者**：Matsumoto S, et al.
**摘要**：通过X射线晶体学解析了RNPEP重组蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基。实验表明，RNPEP对N端精氨酸残基具有高度选择性，这一发现有助于设计特异性抑制剂以干预相关病理过程。
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3. **文献名称**：*"RNPEP Knockdown Attenuates Renal Fibrosis via Modulating Angiotensin Peptide Metabolism"*
**作者**：Zhang Y, et al.
**摘要**：利用RNPEP重组蛋白进行体外细胞实验，发现抑制RNPEP表达可减少血管紧张素III的生成，进而缓解TGF-β诱导的肾小管细胞纤维化。提示RNPEP可能成为慢性肾脏病的潜在治疗靶点。
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如需进一步扩展，可补充疾病关联或药物筛选方向的研究文献。
**Background of RNPEP Recombinant Protein**
RNPEP (arginyl aminopeptidase), also known as aminopeptidase B, is a zinc-dependent metalloprotease belonging to the M1 family of peptidases. It plays a critical role in the cleavage of N-terminal arginine or lysine residues from bioactive peptides, regulating their activity, stability, and receptor interactions. This enzyme is involved in diverse physiological processes, including blood pressure modulation, immune response, and neuropeptide metabolism. For instance, RNPEP processes angiotensin III, a key peptide in the renin-angiotensin system, influencing cardiovascular homeostasis. It also participates in antigen presentation by trimming peptides for major histocompatibility complex (MHC) class I molecules.
The recombinant form of RNPEP is engineered using biotechnological methods, typically expressed in bacterial or mammalian systems to ensure proper folding and enzymatic activity. Recombinant RNPEP retains the catalytic domain and substrate specificity of the native enzyme, enabling its use in biochemical assays, drug discovery, and functional studies. Its production involves cloning the RNPEP gene into expression vectors, followed by purification via affinity chromatography.
Research on RNPEP recombinant protein has expanded due to its potential biomedical applications. Abnormal RNPEP activity is linked to diseases such as hypertension, cancer, and autoimmune disorders, making it a target for therapeutic intervention. Additionally, recombinant RNPEP serves as a tool to study enzyme kinetics, inhibitor screening, and peptide metabolism pathways. Its role in modulating bioactive peptides underscores its importance in both basic research and translational medicine, offering insights into novel treatment strategies for peptide-mediated pathologies.
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