| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MMP24 |
| Uniprot No | Q9Y5R2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-645aa |
| 氨基酸序列 | MPRSRGGRAAPGPPPPPPPPGQAPRWSRWRVPGRLLLLLLPALCCLPGAARAAAAAAGAGNRAAVAVAVARADEAEAPFAGQNWLKSYGYLLPYDSRASALHSAKALQSAVSTMQQFYGIPVTGVLDQTTIEWMKKPRCGVPDHPHLSRRRRNKRYALTGQKWRQKHITYSIHNYTPKVGELDTRKAIRQAFDVWQKVTPLTFEEVPYHEIKSDRKEADIMIFFASGFHGDSSPFDGEGGFLAHAYFPGPGIGGDTHFDSDEPWTLGNANHDGNDLFLVAVHELGHALGLEHSSDPSAIMAPFYQYMETHNFKLPQDDLQGIQKIYGPPAEPLEPTRPLPTLPVRRIHSPSERKHERQPRPPRPPLGDRPSTPGTKPNICDGNFNTVALFRGEMFVFKDRWFWRLRNNRVQEGYPMQIEQFWKGLPARIDAAYERADGRFVFFKGDKYWVFKEVTVEPGYPHSLGELGSCLPREGIDTALRWEPVGKTYFFKGERYWRYSEERRATDPGYPKPITVWKGIPQAPQGAFISKEGYYTYFYKGRDYWKFDNQKLSVEPGYPRNILRDWMGCNQKEVERRKERRLPQDDVDIMVTINDVPGSVNAVAVVIPCILSLCILVLVYTIFQFKNKTGPQPVTYYKRPVQEWV |
| 预测分子量 | 73,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MMP24重组蛋白的3篇参考文献，涵盖其功能、结构及疾病关联研究：
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1. **文献名称**：*MT5-MMP promotes cancer cell migration and invasion through extracellular matrix degradation in vitro*
**作者**：Seiki, M., et al.
**摘要**：该研究利用重组MMP24(MT5-MMP)蛋白，证明其在体外通过降解胶原和明胶等细胞外基质成分，促进癌细胞的迁移和侵袭，提示其在肿瘤转移中的潜在作用。
2. **文献名称**：*Structural insights into the catalytic domain of human MMP24 and its interaction with inhibitors*
**作者**：Morgunova, E., et al.
**摘要**：通过重组MMP24蛋白的结晶和结构分析，揭示了其催化结构域的三维构象，并探讨了特异性抑制剂的设计策略，为靶向治疗提供结构基础。
3. **文献名称**：*MMP24 modulates amyloid precursor protein processing in neuronal cells*
**作者**：Cheret, C., et al.
**摘要**：研究发现重组MMP24能够切割淀粉样前体蛋白(APP)，影响β-淀粉样蛋白生成，提示其在阿尔茨海默病病理中的潜在调控机制。
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*注：上述文献为示例性质，实际文献需通过学术数据库验证。建议使用PubMed或Google Scholar搜索“MMP24 recombinant protein”获取最新研究。*
**Background of MMP24 Recombinant Protein**
Matrix Metalloproteinase 24 (MMP24), also known as MT5-MMP, is a member of the matrix metalloproteinase family, a group of zinc-dependent endopeptidases involved in extracellular matrix (ECM) remodeling and tissue homeostasis. MMP24 is classified as a membrane-type MMP (MT-MMP) due to its transmembrane domain, which anchors it to the cell surface. It is synthesized as an inactive zymogen (pro-MMP24) that requires proteolytic cleavage for activation, typically mediated by furin or other proprotein convertases.
Functionally, MMP24 plays roles in ECM degradation, cell migration, and signaling by processing substrates such as collagen, laminin, and proteoglycans. It also regulates bioactive molecules like growth factors and adhesion proteins, influencing processes like neurodevelopment, wound healing, and immune responses. Notably, MMP24 is highly expressed in the central nervous system, where it may contribute to neuronal plasticity and neurodegenerative diseases, including Alzheimer’s, through interactions with amyloid precursor protein.
In pathological contexts, MMP24 is implicated in cancer progression, particularly in tumor invasion and metastasis, by facilitating ECM breakdown and modulating the tumor microenvironment. Its dysregulation has also been linked to inflammatory disorders and fibrosis.
Recombinant MMP24 protein is engineered for research and therapeutic applications. Produced via heterologous expression systems (e.g., mammalian or bacterial cells), the purified protein retains enzymatic activity and is utilized to study MMP24’s biological mechanisms, screen inhibitors, or model disease processes *in vitro*. Quality-controlled batches ensure consistency in activity assays, structural studies, and drug development efforts. Research on MMP24 continues to explore its dual roles in health and disease, highlighting its potential as a diagnostic marker or therapeutic target.
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