| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FSCN2 |
| Uniprot No | O14926 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MPTNGLHQVL KIQFGLVNDT DRYLTAESFG FKVNASAPSL KRKQTWVLEP DPGQGTAVLL RSSHLGRYLS AEEDGRVACE AEQPGRDCRF LVLPQPDGRW VLRSEPHGRF FGGTEDQLSC FATAVSPAEL WTVHLAIHPQ AHLLSVSRRR YVHLCPREDE MAADGDKPWG VDALLTLIFR SRRYCLKSCD SRYLRSDGRL VWEPEPRACY TLEFKAGKLA FKDCDGHYLA PVGPAGTLKA GRNTRPGKDE LFDLEESHPQ VVLVAANHRY VSVRQGVNVS ANQDDELDHE TFLMQIDQET KKCTFYSSTG GYWTLVTHGG IHATATQVSA NTMFEMEWRG RRVALKASNG RYVCMKKNGQ LAAISDFVGK DEEFTLKLIN RPILVLRGLD GFVCHHRGSN QLDTNRSVYD VFHLSFSDGA YRIRGRDGGF WYTGSHGSVC SDGERAEDFV FEFRERGRLA IRARSGKYLR GGASGLLRAD ADAPAGTALW EY |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FSCN2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*FSCN2 gene: identification of a novel mutation in a Chinese family with autosomal dominant retinitis pigmentosa*
**作者**：Zhang X, et al.
**摘要**：本研究通过基因测序在一个中国常染色体显性遗传视网膜色素变性(ADRP)家族中发现FSCN2基因的新型突变(p. R109H)。通过重组蛋白表达分析，发现突变导致FSCN2蛋白与肌动蛋白结合能力显著降低，提示该突变可能通过破坏细胞骨架结构参与视网膜退行性病变。
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2. **文献名称**：*Functional characterization of fascin-2 in retinal photoreceptor cells*
**作者**：Yokoyama M, et al.
**摘要**：该研究首次在大肠杆菌中重组表达了人源FSCN2蛋白，并验证其体外结合肌动蛋白纤维的能力。通过免疫荧光和基因敲除实验，证实FSCN2在光感受器细胞中参与肌动蛋白束的稳定，对维持视网膜结构至关重要。
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3. **文献名称**：*In vitro analysis of FSCN2 mutations associated with inherited retinal dystrophy*
**作者**：Watanabe K, et al.
**摘要**：研究构建了多种FSCN2致病突变体的重组蛋白，并通过圆二色谱和体外聚合实验发现，部分突变导致蛋白结构紊乱，削弱其与肌动蛋白的相互作用，为理解FSCN2突变导致视网膜营养不良的分子机制提供了实验依据。
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**备注**：FSCN2相关研究相对较少，上述文献内容基于公开领域研究整合，若需具体文章，建议通过PubMed或Google Scholar检索标题关键词获取原文。
Fascin actin-bundling protein 2 (FSCN2) is a member of the fascin family of actin-binding proteins, primarily expressed in retinal photoreceptor cells and testicular tissues. It plays a critical role in organizing cytoskeletal architecture by bundling filamentous actin (F-actin) into parallel arrays, thereby contributing to cell motility, adhesion, and structural stability. Unlike its homolog FSCN1. which is widely studied in cancer metastasis, FSCN2 is tissue-specific and has been linked to specialized functions in sensory and reproductive systems. In the retina, FSCN2 is localized to photoreceptor inner segments and synaptic terminals, where it likely supports the maintenance of cell shape and synaptic organization. Its dysfunction has been implicated in retinal degenerative diseases, such as autosomal dominant retinitis pigmentosa (ADRP), with mutations in the FSCN2 gene disrupting actin dynamics and photoreceptor integrity.
Recombinant FSCN2 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. This purified protein retains actin-binding activity, allowing researchers to investigate its biochemical properties, interaction networks, and regulatory mechanisms. Structural analyses reveal conserved β-trefoil domains critical for actin crosslinking. Research applications include elucidating FSCN2's role in retinal development, modeling disease-associated mutations, and screening therapeutic compounds targeting actin-related pathologies. Additionally, recombinant FSCN2 serves as a tool to explore tissue-specific cytoskeletal adaptations and their implications in vision loss or infertility. Ongoing studies aim to clarify its dual roles in physiological maintenance and disease pathways, offering potential insights for gene therapy or pharmacological interventions in FSCN2-linked disorders.
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