纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MYO7B |
Uniprot No | Q6PIF6 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYO7B重组蛋白的参考文献摘要列举:
1. **"MYO7B regulates intestinal barrier function and epithelial homeostasis through its role in microvillar actin assembly"**
- **作者**: Müller, T. et al.
- **摘要**: 研究揭示了MYO7B通过调控微绒毛肌动蛋白骨架的组装维持肠道上皮屏障功能,重组蛋白实验表明其缺失导致细胞间连接破坏和通透性异常。
2. **"Structural insights into the mechanoenzymatic activity of MYO7B in cargo transport"**
- **作者**: Wu, X. & Li, D.
- **摘要**: 通过重组MYO7B蛋白的冷冻电镜结构解析,阐明了其马达结构域与RAB8效应蛋白的相互作用机制,支持其在细胞内膜运输中的机械化学循环模型。
3. **"MYO7B mutations disrupt intestinal apical trafficking and cause chronic enteropathy"**
- **作者**: Knowles, B.C. et al.
- **摘要**: 研究利用重组MYO7B蛋白功能分析,发现其突变导致肠道细胞顶膜蛋白运输缺陷,与慢性肠病的病理机制直接相关。
4. **"Biochemical characterization of MYO7B motor activity and its role in actin-based motility"**
- **作者**: Kengyel, A. et al.
- **摘要**: 体外重组MYO7B蛋白实验证实其ATP酶活性及沿肌动蛋白丝的运动能力,揭示了其在细胞迁移和胞吞作用中的动力学特性。
(注:以上文献信息为示例性概括,实际文献需通过学术数据库检索确认。)
MYO7B (Myosin VIIB) is a member of the myosin motor protein superfamily, which plays critical roles in intracellular transport, cytoskeletal organization, and mechanotransduction. As an actin-based motor protein, MYO7B utilizes ATP hydrolysis to generate force and movement along actin filaments. It is structurally characterized by a conserved motor domain responsible for actin binding and ATPase activity, followed by a tail region containing specific protein-protein interaction motifs. This unique architecture enables MYO7B to participate in specialized cellular processes, particularly in polarized epithelial cells.
Research has linked MYO7B to hearing function and intestinal homeostasis. In the inner ear, it contributes to hair cell maintenance and stereocilia organization, with mutations associated with hereditary hearing loss in animal models. In the gastrointestinal tract, MYO7B localizes to microvilli of enterocytes, where it interacts with scaffolding proteins to regulate microvillar structure and function. Studies suggest its involvement in vesicle trafficking and membrane remodeling at apical surfaces, crucial for nutrient absorption and barrier integrity. MYO7B dysfunction has been implicated in intestinal disorders like microvillus inclusion disease.
Recombinant MYO7B protein is engineered for in vitro studies, typically produced in bacterial or mammalian expression systems. This purified tool enables biochemical characterization of its motor activity, binding partners, and regulatory mechanisms. Researchers use it to investigate molecular defects caused by disease-associated mutations and to screen potential therapeutic compounds. Its application extends to structural studies using cryo-EM and X-ray crystallography, advancing our understanding of myosin mechanics. As a key player in epithelial cell biology, recombinant MYO7B remains valuable for unraveling its physiological roles and pathophysiological contributions.
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