| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL23A |
| Uniprot No | P62750 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-156aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAPKAKK EAPAPPKAEA KAKALKAKKA VLKGVHSHKK KKIRTSPTFR RPKTLRLRRQ PKYPRKSAPR RNKLDHYAII KFPLTTESAM KKIEDNNTLV FIVDVKANKH QIKQAVKKLY DIDVAKVNTL IRPDGEKKAY VRLAPDYDAL DVANKIGII |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL23A重组蛋白的参考文献示例(注：以下为模拟内容，实际文献需根据具体数据库检索验证)：
---
1. **标题**: *"Heterologous Expression and Functional Characterization of Human RPL23A in Saccharomyces cerevisiae"*
**作者**: Müller S, et al.
**摘要**: 研究利用酵母系统重组表达人源RPL23A，验证其替代酵母内源同源蛋白的能力，并揭示其在核糖体生物合成中的保守功能。
2. **标题**: *"Purification and Crystallographic Analysis of Recombinant RPL23A from Arabidopsis thaliana"*
**作者**: Kim D, Lee J.
**摘要**: 报道拟南芥RPL23A的重组表达与纯化流程，通过晶体结构分析揭示其与RNA结合的潜在位点，为核糖体结构研究提供基础。
3. **标题**: *"RPL23A Recombinant Protein Suppresses Tumor Growth by Modulating mTOR Signaling in Colorectal Cancer Models"*
**作者**: Zhang H, et al.
**摘要**: 探索重组RPL23A蛋白在结直肠癌细胞中的功能，证明其通过抑制mTOR通路降低肿瘤增殖，提示其作为治疗靶点的潜力。
4. **标题**: *"Development of a High-Yield E. coli System for Recombinant RPL23A Production: Applications in Antibiotic Resistance Studies"*
**作者**: Gupta R, et al.
**摘要**: 优化大肠杆菌表达体系以提高RPL23A产量，并利用纯化蛋白研究其与抗生素(如环己酰亚胺)的相互作用机制。
---
**提示**：实际文献需通过PubMed、Google Scholar等平台检索确认。建议使用关键词“RPL23A recombinant expression”、“RPL23A purification”或结合具体研究领域(如癌症、结构生物学)进行筛选。
RPL23A (Ribosomal Protein L23A) is a component of the 60S ribosomal subunit, playing a critical role in ribosome biogenesis and protein synthesis. As part of the L23A family of ribosomal proteins, it contributes to the structural stability of the large ribosomal subunit and facilitates interactions during translation initiation and elongation. Beyond its canonical role, RPL23A has been implicated in extra-ribosomal functions, including cell cycle regulation, apoptosis, and DNA repair, highlighting its potential involvement in cellular stress responses and oncogenesis.
Recombinant RPL23A protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper folding and post-translational modifications. The recombinant form allows researchers to study its biochemical properties, protein-protein interactions, and regulatory mechanisms in controlled environments. Its production often involves affinity tags (e.g., His-tag, GST) for purification and detection, enabling applications in structural studies (X-ray crystallography, NMR), *in vitro* translation assays, and functional genomics.
Interest in RPL23A has grown due to its dysregulation in cancers, such as hepatocellular carcinoma and leukemia, where overexpression correlates with tumor progression and chemoresistance. Recombinant RPL23A serves as a tool to investigate its role in ribosomopathies and cancer biology, including interactions with oncogenic signaling pathways like p53-MDM2. Additionally, studies explore its potential as a therapeutic target or biomarker. Challenges remain in elucidating its dual ribosomal and non-ribosomal functions, necessitating further research using recombinant protein-based models to unravel its multifaceted roles in health and disease.
×
