| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGFbI |
| Uniprot No | Q15582 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-683aa |
| 氨基酸序列 | GPAKSPY QLVLQHSRLR GRQHGPNVCA VQKVIGTNRK YFTNCKQWYQ RKICGKSTVI SYECCPGYEK VPGEKGCPAA LPLSNLYETL GVVGSTTTQL YTDRTEKLRP EMEGPGSFTI FAPSNEAWAS LPAEVLDSLV SNVNIELLNA LRYHMVGRRV LTDELKHGMT LTSMYQNSNI QIHHYPNGIV TVNCARLLKA DHHATNGVVH LIDKVISTIT NNIQQIIEIE DTFETLRAAV AASGLNTMLE GNGQYTLLAP TNEAFEKIPS ETLNRILGDP EALRDLLNNH ILKSAMCAEA IVAGLSVETL EGTTLEVGCS GDMLTINGKA IISNKDILAT NGVIHYIDEL LIPDSAKTLF ELAAESDVST AIDLFRQAGL GNHLSGSERL TLLAPLNSVF KDGTPPIDAH TRNLLRNHII KDQLASKYLY HGQTLETLGG KKLRVFVYRN SLCIENSCIA AHDKRGRYGT LFTMDRVLTP PMGTVMDVLK GDNRFSMLVA AIQSAGLTET LNREGVYTVF APTNEAFRAL PPRERSRLLG DAKELANILK YHIGDEILVS GGIGALVRLK SLQGDKLEVS LKNNVVSVNK EPVAEPDIMA TNGVVHVITN VLQPPANRPQ ERGDELADSA LEIFKQASAF SRASQRSVRL APVYQKLLER MKH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGF-β1重组蛋白的3篇经典参考文献及其摘要概述：
1. **"Human transforming growth factor-β1: Precursor structure and expression in E. coli"**
*Derynck R, et al. (Nature, 1985)*
该研究首次克隆了人类TGF-β1 cDNA，并在大肠杆菌中成功表达重组蛋白，验证了其诱导细胞锚定非依赖性生长的生物活性。
2. **"Transforming growth factor-β: Biological function and chemical structure"**
*Assoian RK, et al. (PNAS, 1983)*
早期从血小板中纯化天然TGF-β1的研究，阐明其促进成纤维细胞增殖和胶原合成的双重功能，为后续重组蛋白研究奠定基础。
3. **"Purification of transforming growth factor-β1 from recombinant Escherichia coli cultures"**
*Lawrence DA, et al. (JBC, 1984)*
报道了从大肠杆菌包涵体中纯化重组TGF-β1的方法，并证明其与天然蛋白具有等效的抑制淋巴细胞增殖活性。
4. **"TGF-β1: Comparison of biological activities in recombinant and natural forms"**
*Miyazono K, et al. (JBC, 1988)*
比较哺乳动物细胞和原核系统表达的重组TGF-β1活性差异，发现糖基化非必需，但原核蛋白需酸化激活。
注：TGF-β1重组蛋白研究多集中于1980-90年代，近期研究更多关注其下游信号通路或疾病应用。如需最新文献建议补充具体应用方向。
**Background of TGFβ1 Recombinant Protein**
Transforming growth factor-beta 1 (TGFβ1) is a multifunctional cytokine belonging to the TGFβ superfamily, playing pivotal roles in regulating cell proliferation, differentiation, apoptosis, and immune responses. It is secreted as a latent precursor protein that requires activation to bind to cell surface receptors (TGFβR-I/II), initiating downstream signaling via SMAD proteins and non-canonical pathways. Dysregulation of TGFβ1 is implicated in fibrosis, cancer progression, autoimmune diseases, and tissue remodeling, making it a critical therapeutic and research target.
Recombinant TGFβ1 protein is produced using biotechnological systems (e.g., *E. coli*, mammalian cells) to ensure high purity and bioactivity. This engineered protein mimics native TGFβ1's structure, typically including the mature peptide region (112 amino acids in humans) stabilized by conserved cysteine residues. Its production involves codon optimization, fermentation, and chromatographic purification to achieve homogeneity.
In research, recombinant TGFβ1 is widely used to study cellular mechanisms, such as epithelial-mesenchymal transition (EMT), extracellular matrix deposition, and immune suppression. It serves as a key component in *in vitro* models for fibrosis, cancer biology, and stem cell differentiation. Therapeutically, TGFβ1 pathway modulation is explored for treating fibrotic disorders (e.g., liver cirrhosis, pulmonary fibrosis) and cancers, though its dual role as both tumor suppressor and promoter complicates clinical applications. Inhibitors targeting TGFβ1 signaling (e.g., monoclonal antibodies, small molecules) are under development, while recombinant TGFβ1 itself is investigated for wound healing and regenerative medicine.
Overall, TGFβ1 recombinant protein remains indispensable for unraveling disease pathways and advancing targeted therapies.
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