| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSDC2 |
| Uniprot No | Q9Y534 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMTSESTS PPVVPPLHSP KSPVWPTFPF HREGSRVWER GGVPPRDLPS PLPTKRTRTY SATARASAGP VFKGVCKQFS RSQGHGFITP ENGSEDIFVH VSDIEGEYVP VEGDEVTYKM CPIPPKNQKF QAVEVVLTQL APHTPHETWS GQVVGS |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于公开文献数据库的模拟示例(请注意，CSDC2相关研究较少，部分内容可能为示意性概述)：
1. **"Expression and functional characterization of recombinant CSDC2 in E. coli"**
*作者：Li, X., et al. (2018)*
摘要：本研究成功在大肠杆菌中表达了可溶性CSDC2重组蛋白，通过镍柱亲和层析纯化，并证实其具有体外结合特定RNA序列的能力，为后续功能研究奠定基础。
2. **"CSDC2 modulates tumor metastasis via interaction with lncRNAs"**
*作者：Wang, Y., et al. (2020)*
摘要：利用昆虫细胞系统表达CSDC2重组蛋白，结合Pull-down实验发现其与多种长链非编码RNA互作，可能通过调控EMT通路影响癌症转移。
3. **"Structural insights into CSDC2's cold-shock domain by X-ray crystallography"**
*作者：Tanaka, K., et al. (2021)*
摘要：通过杆状病毒系统表达并纯化人源CSDC2重组蛋白，解析其冷休克结构域的三维结构，揭示其核酸结合位点的关键氨基酸残基。
4. **"CSDC2 recombinant protein attenuates oxidative stress in neuronal cells"**
*作者：Gupta, R., & Lee, S. (2019)*
摘要：哺乳动物细胞表达的CSDC2重组蛋白在体外模型中显示出抗氧化活性，可能通过激活Nrf2通路保护神经元免受ROS损伤。
**注意**：以上为学术写作示例，实际研究中CSDC2(Cold Shock Domain Containing C2)的公开文献较为有限，建议通过PubMed/Google Scholar结合基因别名(如RBM44)进行扩展检索，并验证最新研究进展。
CSDC2 (Cold Shock Domain Containing C2), also known as RBM24 (RNA-Binding Motif Protein 24), is a RNA-binding protein that plays critical roles in post-transcriptional gene regulation. It belongs to the cold shock domain (CSD) protein family, characterized by a conserved nucleic acid-binding domain involved in RNA recognition and interactions. CSDC2 is particularly noted for its regulatory functions in cell differentiation, embryonic development, and tissue-specific gene expression. Studies highlight its importance in cardiac and skeletal muscle development, where it modulates mRNA stability, splicing, and translation of target transcripts.
Recombinant CSDC2 protein is engineered to enable in vitro studies of its molecular mechanisms. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant form retains functional RNA-binding activity and structural integrity. Purification typically involves affinity tags (e.g., His-tag) and chromatography techniques. Researchers utilize recombinant CSDC2 to investigate its interactions with RNA substrates, role in cellular stress responses, and involvement in diseases such as cardiomyopathy, muscular dystrophy, or cancer, where its dysregulation has been implicated.
Recent work also explores CSDC2’s potential in regenerative medicine due to its influence on stem cell differentiation. Its ability to regulate alternative splicing events and stabilize mRNAs during cellular transitions makes it a key target for therapeutic strategies. Despite progress, full elucidation of its binding partners, signaling networks, and tissue-specific functions remains an active area of research, driving demand for high-purity recombinant CSDC2 in both basic and applied biomedical studies.
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