| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CK1 |
| Uniprot No | P04264 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-644aa |
| 氨基酸序列 | SRQFSSRSGYRSGGGFSSGSAGIINYQRRTTSSSTRRSGGGGGRFSSCGGGGGSFGAGGGFGSRSLVNLGGSKSISISVARGGGRGSGFGGGYGGGGFGGGGFGGGGFGGGGIGGGGFGGFGSGGGGFGGGGFGGGGYGGGYGPVCPPGGIQEVTINQSLLQPLNVEIDPEIQKVKSREREQIKSLNNQFASFIDKVRFLEQQNQVLQTKWELLQQVDTSTRTHNLEPYFESFINNLRRRVDQLKSDQSRLDSELKNMQDMVEDYRNKYEDEINKRTNAENEFVTIKKDVDGAYMTKVDLQAKLDNLQQEIDFLTALYQAELSQMQTQISETNVILSMDNNRSLDLDSIIAEVKAQYEDIAQKSKAEAESLYQSKYEELQITAGRHGDSVRNSKIEISELNRVIQRLRSEIDNVKKQISNLQQSISDAEQRGENALKDAKNKLNDLEDALQQAKEDLARLLRDYQELMNTKLALDLEIATYRTLLEGEESRMSGECAPNVSVSVSTSHTTISGGGSRGGGGGGYGSGGSSYGSGGGSYGSGGGGGGGRGSYGSGGSSYGSGGGSYGSGGGGGGHGSYGSGSSSGGYRGGSGGGGGGSSGGRGSGGGSSGGSIGGRGSSSGGVKSSGGSSSVKFVSTTYSGVTR |
| 预测分子量 | 69.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于CK1重组蛋白的虚构参考文献示例，格式包含文献名称、作者及摘要概括：
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1. **文献名称**: *"High-yield Production of Recombinant Human CK1δ in Insect Cells and Its Biochemical Characterization"*
**作者**: Müller, T. et al.
**摘要**: 研究利用杆状病毒-昆虫细胞系统高效表达人源CK1δ重组蛋白，优化纯化步骤获得高活性激酶，并验证其在体外磷酸化底物β-catenin的能力。
2. **文献名称**: *"Recombinant CK1ε as a Tool for Studying Circadian Rhythm Regulation in Mammalian Cells"*
**作者**: Chen, Y. & Rossi, F.
**摘要**: 通过大肠杆菌表达重组CK1ε蛋白，探究其与PER2蛋白的相互作用机制，揭示CK1ε在生物钟调控中的磷酸化作用及动力学特征。
3. **文献名称**: *"Crystal Structure of Recombinant CK1α Bound to a ATP-competitive Inhibitor"*
**作者**: Sato, K. et al.
**摘要**: 解析重组CK1α与抑制剂复合物的晶体结构，阐明其激酶域结合模式，为靶向CK1α的药物设计提供结构基础。
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(注：以上文献为示例，实际引用需查询具体数据库如PubMed、Web of Science等。)
**Background of CK1 Recombinant Proteins**
Casein Kinase 1 (CK1) is a conserved family of serine/threonine protein kinases involved in diverse cellular processes, including cell cycle regulation, DNA repair, signal transduction, and circadian rhythm control. Members of the CK1 family, such as CK1α, CK1γ, CK1δ, and CK1ε, share a conserved kinase domain but exhibit distinct substrate specificities and regulatory mechanisms due to divergent N- or C-terminal regions. Dysregulation of CK1 isoforms has been linked to diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, making them attractive therapeutic targets.
Recombinant CK1 proteins are engineered using expression systems like *E. coli*, insect cells, or mammalian cells to ensure proper folding and post-translational modifications. These proteins are purified via affinity tags (e.g., His-tag) and validated for enzymatic activity using kinase assays. Recombinant CK1 serves as a critical tool for *in vitro* studies to decipher substrate interactions, screen inhibitors, or analyze structure-function relationships. For example, CK1δ/ε inhibitors are being explored for cancer therapy, while CK1α modulators are studied in Wnt signaling-related diseases.
Structural studies of recombinant CK1 have revealed autoinhibitory mechanisms and substrate-binding pockets, guiding drug design. Despite progress, challenges remain in isoform-specific targeting due to high structural similarity. Ongoing research focuses on optimizing recombinant CK1 production and developing selective inhibitors, leveraging advances in structural biology and high-throughput screening.
In summary, recombinant CK1 proteins are indispensable for understanding kinase biology and advancing translational research, bridging the gap between mechanistic insights and therapeutic applications.
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