| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD21 |
| Uniprot No | P20023-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-971aa |
| 氨基酸序列 | MGAAGLLGVF LALVAPGVLG ISCGSPPPIL NGRISYYSTP IAVGTVIRYS CSGTFRLIGE KSLLCITKDK VDGTWDKPAP KCEYFNKYSS CPEPIVPGGY KIRGSTPYRH GDSVTFACKT NFSMNGNKSV WCQANNMWGP TRLPTCVSVF PLECPALPMI HNGHHTSENV GSIAPGLSVT YSCESGYLLV GEKIINCLSS GKWSAVPPTC EEARCKSLGR FPNGKVKEPP ILRVGVTANF FCDEGYRLQG PPSSRCVIAG QGVAWTKMPV CEEIFCPSPP PILNGRHIGN SLANVSYGSI VTYTCDPDPE EGVNFILIGE STLRCTVDSQ KTGTWSGPAP RCELSTSAVQ CPHPQILRGR MVSGQKDRYT YNDTVIFACM FGFTLKGSKQ IRCNAQGTWE PSAPVCEKEC QAPPNILNGQ KEDRHMVRFD PGTSIKYSCN PGYVLVGEES IQCTSEGVWT PPVPQCKVAA CEATGRQLLT KPQHQFVRPD VNSSCGEGYK LSGSVYQECQ GTIPWFMEIR LCKEITCPPP PVIYNGAHTG SSLEDFPYGT TVTYTCNPGP ERGVEFSLIG ESTIRCTSND QERGTWSGPA PLCKLSLLAV QCSHVHIANG YKISGKEAPY FYNDTVTFKC YSGFTLKGSS QIRCKADNTW DPEIPVCEKE TCQHVRQSLQ ELPAGSRVEL VNTSCQDGYQ LTGHAYQMCQ DAENGIWFKK IPLCKVIHCH PPPVIVNGKH TGMMAENFLY GNEVSYECDQ GFYLLGEKKL QCRSDSKGHG SWSGPSPQCL RSPPVTRCPN PEVKHGYKLN KTHSAYSHND IVYVDCNPGF IMNGSRVIRC HTDNTWVPGV PTCIKKAFIG CPPPPKTPNG NHTGGNIARF SPGMSILYSC DQGYLLVGEA LLLCTHEGTW SQPAPHCKEV NCSSPADMDG IQKGLEPRKM YQYGAVVTLE CEDGYMLEGS PQSQCQSDHQ WNPPLAVCRS R |
| 预测分子量 | 106 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD21重组蛋白的3篇示例参考文献(内容为虚构示例，仅供格式参考)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant CD21 in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：研究报道了在HEK293细胞中成功表达重组CD21蛋白，并验证其与补体片段C3d的结合活性，证实其在B细胞受体共刺激中的功能。
2. **文献名称**：*Structural Analysis of CD21 Using Recombinant Protein Crystallography*
**作者**：Lee H, et al.
**摘要**：通过重组CD21蛋白的晶体结构解析，揭示了其与EB病毒gp350蛋白的相互作用界面，为抗病毒药物设计提供结构基础。
3. **文献名称**：*Recombinant CD21 as a Tool for Studying Immune Complex Formation*
**作者**：Garcia R, et al.
**摘要**：利用重组CD21蛋白探究其在免疫复合物捕获中的作用，证明其通过结合C3d增强抗原呈递效率，为疫苗佐剂开发提供依据。
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**注意**：以上文献信息为模拟内容，实际研究中请通过PubMed或学术数据库(如Google Scholar)检索真实文献，并核实原文内容。
CD21. also known as complement receptor type 2 (CR2), is a cell-surface glycoprotein primarily expressed on B lymphocytes and follicular dendritic cells. It functions as a receptor for complement component C3d, linking innate and adaptive immunity by enhancing B cell activation upon antigen recognition. Structurally, CD21 consists of 15 or 16 short consensus repeat (SCR) extracellular domains, a transmembrane region, and a cytoplasmic tail. Its interaction with C3d-opsonized antigens or immune complexes facilitates co-stimulatory signals alongside the B cell receptor (BCR), promoting antibody production and immune memory.
Recombinant CD21 protein is engineered through molecular cloning, typically using mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications. The purified protein often retains functional epitopes of the extracellular domain, making it valuable for studying B cell biology, complement system interactions, and immune complex trafficking. Researchers utilize CD21 recombinant proteins to investigate mechanisms in autoimmune diseases (e.g., lupus), immunodeficiency disorders, and viral infections (e.g., Epstein-Barr virus and HIV), as CD21 serves as an entry co-receptor for some pathogens.
In therapeutic development, recombinant CD21 aids in designing targeted immunotherapies and vaccines by mimicking natural ligand-binding properties. It also serves as a critical tool for structural studies, antibody validation, and high-throughput drug screening. Recent applications explore its role in modulating excessive complement activation in inflammatory conditions. As a standardized reagent, recombinant CD21 enhances reproducibility in both basic research and clinical assay development, particularly in autoimmune diagnostics and B cell-related cancer studies.
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