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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANG3 |
| Uniprot No | O95841 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-491aa |
| 氨基酸序列 | GQFKIKK INQRRYPRAT DGKEEAKKCA YTFLVPEQRI TGPICVNTKG QDASTIKDMI TRMDLENLKD VLSRQKREID VLQLVVDVDG NIVNEVKLLR KESRNMNSRV TQLYMQLLHE IIRKRDNSLE LSQLENKILN VTTEMLKMAT RYRELEVKYA SLTDLVNNQS VMITLLEEQC LRIFSRQDTH VSPPLVQVVP QHIPNSQQYT PGLLGGNEIQ RDPGYPRDLM PPPDLATSPT KSPFKIPPVT FINEGPFKDC QQAKEAGHSV SGIYMIKPEN SNGPMQLWCE NSLDPGGWTV IQKRTDGSVN FFRNWENYKK GFGNIDGEYW LGLENIYMLS NQDNYKLLIE LEDWSDKKVY AEYSSFRLEP ESEFYRLRLG TYQGNAGDSM MWHNGKQFTT LDRDKDMYAG NCAHFHKGGW WYNACAHSNL NGVWYRGGHY RSKHQDGIFW AEYRGGSYSL RAVQMMIKPI D |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANGPTL3(Angiopoietin-like 3)重组蛋白的3篇参考文献示例，涵盖其功能、表达及应用研究：
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1. **文献名称**: *ANGPTL3 regulates lipoprotein metabolism and atherosclerosis through LPL and endothelial lipase-dependent mechanisms*
**作者**: Gusarova V. et al.
**摘要**: 研究通过重组ANGPTL3蛋白实验，揭示了其通过抑制脂蛋白脂肪酶(LPL)和内皮脂肪酶(EL)调控脂代谢的机制，并证明其缺失可降低动脉粥样硬化风险。
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2. **文献名称**: *Production and characterization of recombinant human ANGPTL3 for functional studies*
**作者**: Yau M.H. et al.
**摘要**: 描述了重组人ANGPTL3蛋白在大肠杆菌中的表达与纯化方法，验证其结构与生物活性，为后续靶向治疗高脂血症提供实验基础。
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3. **文献名称**: *Therapeutic targeting of ANGPTL3 with monoclonal antibodies in metabolic disorders*
**作者**: Dewey F.E. et al.
**摘要**: 利用重组ANGPTL3蛋白筛选单克隆抗体，发现其可有效降低血液甘油三酯和胆固醇水平，为代谢综合征治疗提供新策略。
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**备注**：以上文献为示例，实际文献需通过PubMed或学术数据库检索(关键词：ANGPTL3 recombinant protein)。如需具体文章，可提供PMID/DOI进一步定位。
ANG3 recombinant protein, commonly referred to as Angiopoietin-3 or ANGPTL3 (Angiopoietin-like protein 3), is a secretory glycoprotein belonging to the angiopoietin family. It plays a multifaceted role in lipid metabolism and vascular regulation. Structurally, ANGPTL3 contains an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain, which mediate its biological functions. The protein is primarily synthesized in the liver and secreted into the bloodstream, where it acts as a key regulator of lipid homeostasis. It inhibits lipoprotein lipase (LPL) and endothelial lipase (EL), enzymes critical for hydrolyzing triglycerides (TG) and phospholipids, thereby modulating plasma lipid levels. Dysregulation of ANGPTL3 is linked to metabolic disorders, including hypolipidemia and atherosclerosis.
The recombinant form of ANGPTL3 is produced using biotechnological platforms, such as mammalian or bacterial expression systems, to ensure high purity and bioactivity. This engineered protein retains the functional domains necessary for research and therapeutic applications. Recent studies highlight its dual role in lipid metabolism and angiogenesis, making it a potential therapeutic target for cardiovascular diseases and rare lipid disorders. For instance, monoclonal antibodies or gene-silencing therapies targeting ANGPTL3 have shown promise in reducing atherogenic lipoproteins in clinical trials.
In research, ANG3 recombinant protein is widely used to study metabolic pathways, endothelial cell interactions, and drug development. Its involvement in both lipid regulation and vascular biology underscores its relevance to precision medicine, particularly for patients with familial hypercholesterolemia or other inherited lipid abnormalities. Ongoing investigations aim to clarify its tissue-specific signaling mechanisms and explore its diagnostic potential as a biomarker for metabolic syndromes.
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