| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCT |
| Uniprot No | P40126 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 24-472aa |
| 氨基酸序列 | QFPRVCMTVDSLVNKECCPRLGAESANVCGSQQGRGQCTEVRADTRPWSGPYILRNQDDRELWPRKFFHRTCKCTGNFAGYNCGDCKFGWTGPNCERKKPPVIRQNIHSLSPQEREQFLGALDLAKKRVHPDYVITTQHWLGLLGPNGTQPQFANCSVYDFFVWLHYYSVRDTLLGPGRPYRAIDFSHQGPAFVTWHRYHLLCLERDLQRLIGNESFALPYWNFATGRNECDVCTDQLFGAARPDDPTLISRNSRFSSWETVCDSLDDYNHLVTLCNGTYEGLLRRNQMGRNSMKLPTLKDIRDCLSLQKFDNPPFFQNSTFSFRNALEGFDKADGTLDSQVMSLHNLVHSFLNGTNALPHSAANDPIFVVLHSFTDAIFDEWMKRFNPPADAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTSDQLGYSYAIDLPVSVEETPGWPTT |
| 预测分子量 | 71.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与DCT(多巴色素异构酶)重组蛋白相关的模拟参考文献示例，供参考：
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1. **文献名称**: "Expression and characterization of recombinant human dopachrome tautomerase (DCT) in Escherichia coli"
**作者**: Jimbow, K. et al.
**摘要**: 研究报道了在大肠杆菌中高效表达可溶性DCT重组蛋白的方法，通过His标签纯化获得高纯度蛋白，并验证其催化多巴色素转化为DHICA的酶活性，为黑色素合成机制研究提供工具。
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2. **文献名称**: "Structural analysis of DCT protein reveals zinc-binding sites critical for enzymatic function"
**作者**: Solano, F. et al.
**摘要**: 利用X射线晶体学解析了重组DCT蛋白的三维结构，发现其锌离子结合位点对酶活性至关重要，突变实验证实特定氨基酸残基参与底物识别，揭示了DCT催化机制的结构基础。
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3. **文献名称**: "DCT knockdown via recombinant siRNA-protein nanoparticles inhibits melanoma progression in vivo"
**作者**: Slominski, A. et al.
**摘要**: 开发了基于DCT重组蛋白的靶向递送系统，将siRNA包裹于蛋白纳米颗粒中，体内实验显示其可有效沉默黑色素瘤细胞的DCT表达，抑制肿瘤生长，为皮肤癌治疗提供新策略。
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注：以上文献信息为模拟生成，实际引用需查询PubMed、Web of Science等数据库获取真实数据。
**Background of DCT Recombinant Proteins**
Recombinant proteins, engineered through genetic modification in host systems like *E. coli* or mammalian cells, play pivotal roles in biomedical research, therapeutics, and diagnostics. DCT (Decorin C-Terminal) recombinant proteins, derived from the C-terminal domain of decorin, a small leucine-rich proteoglycan, are notable for their regulatory functions in extracellular matrix (ECM) organization. Decorin interacts with collagen fibrils via its C-terminal region, influencing fibrillogenesis, tissue remodeling, and cellular signaling pathways. Dysregulation of decorin is linked to fibrosis, cancer progression, and connective tissue disorders, making DCT recombinant proteins valuable tools for studying ECM dynamics and therapeutic targeting.
Alternatively, DCT may refer to dopachrome tautomerase (TYRP2), an enzyme in melanin biosynthesis. This DCT catalyzes the conversion of dopachrome to 5.6-dihydroxyindole-2-carboxylic acid (DHICA), critical for melanin polymer stability. Recombinant DCT proteins aid in studying pigmentation disorders, melanoma mechanisms, and developing depigmentation therapies.
Production of DCT recombinant proteins involves codon optimization, expression in suitable hosts, and purification via affinity chromatography. Challenges include preserving post-translational modifications (e.g., glycosylation in decorin) or enzymatic activity (for TYRP2). Applications span *in vitro* studies, drug screening, and biocompatible materials for tissue engineering.
Overall, DCT recombinant proteins bridge structural biology and translational medicine, offering insights into ECM-related pathologies, melanogenesis, and novel treatment strategies. Their versatility underscores their importance in advancing both basic science and clinical innovation.
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