| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX58 |
| Uniprot No | O95786 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-925aa |
| 氨基酸序列 | TTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK |
| 预测分子量 | 108 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DDX58(RIG-I)重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis for RNA recognition and activation of RIG-I signaling*
**作者**：Gack MU et al.
**摘要**：该研究解析了重组人源RIG-I蛋白的C端结构域(CTD)与病毒RNA结合的晶体结构，揭示了CTD通过识别5'-三磷酸RNA触发RIG-I构象变化，进而激活下游MAVS信号通路的分子机制，为抗病毒药物设计提供了结构基础。
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2. **文献名称**：*Reconstitution of RIG-I-mediated RNA-sensing pathway in vitro*
**作者**：Yoneyama M, Fujita T
**摘要**：研究团队利用重组表达的RIG-I蛋白、MAVS及激酶复合体，在体外重建了病毒RNA诱导的干扰素信号激活通路，证实重组RIG-I可直接结合合成dsRNA并触发级联反应，为高通量筛选RIG-I激动剂提供了实验模型。
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3. **文献名称**：*Targeting RIG-I-like receptors for antiviral immunotherapy*
**作者**：Vazquez C et al.
**摘要**：通过大肠杆菌表达系统制备重组RIG-I蛋白，证明其与工程化RNA配体结合后可增强细胞抗病毒应答，并验证了其在体外培养的免疫细胞中激活干扰素通路的能力，为基于RIG-I的免疫疗法开发提供了依据。
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**备注**：以上文献信息为示例，实际引用时需核对期刊名称、年份及作者全名(如Nature、Cell等期刊相关论文)。建议通过PubMed或Web of Science以“DDX58 recombinant protein”或“RIG-I recombinant”为关键词检索最新研究。
**Background of DDX58 Recombinant Protein**
DDX58. also known as retinoic acid-inducible gene I (RIG-I), is a cytosolic RNA helicase that plays a pivotal role in innate antiviral immunity. As a pattern recognition receptor (PRR), it detects viral RNA, particularly short double-stranded RNA (dsRNA) or 5'-triphosphate-containing single-stranded RNA (ssRNA) derived from invading pathogens. Upon ligand binding, DDX58 undergoes conformational changes, exposing its caspase activation and recruitment domains (CARDs), which interact with the mitochondrial antiviral-signaling protein (MAVS). This interaction triggers downstream signaling cascades, leading to the production of type I interferons (IFNs) and proinflammatory cytokines, essential for antiviral defense.
Recombinant DDX58 protein is engineered in vitro to study its structural and functional properties. It is typically expressed in bacterial, insect, or mammalian cell systems, ensuring proper post-translational modifications and activity. The recombinant protein retains key domains, including the helicase domain for ATP-dependent RNA unwinding and the CARDs for signaling. Researchers utilize it to investigate DDX58’s RNA-binding specificity, activation mechanisms, and interactions with viral RNA or host proteins.
Its applications extend to drug discovery, such as screening for agonists or antagonists to modulate immune responses. Additionally, recombinant DDX58 aids in understanding immune dysregulation in autoimmune diseases or viral evasion strategies. By providing a purified, active form of the protein, it serves as a critical tool for dissecting RIG-I-mediated immunity and developing therapeutic interventions against viral infections or immune disorders.
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