| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PANK4 |
| Uniprot No | O96013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MFGKRKKRVEISAPSNFEHRVHTGFDQHEQKFTGLPRQWQSLIEESARRPKPLVDPACITSIQPGAPKTIVRGSKGAKDGALTLLLDEFENMSVTRSNSLRRDSPPPPARARQENGMPEEPATTARGGPGKAGSRGRFAGHSEAGGGSGDRRRAGPEKRPKSSREGSGGPQESSRDKRPLSGPDVGTPQPAGLASGAKLAAGRPFNTYPRADTDHPSRGAQGEPHDVAPNGPSAGGLAIPQSSSSSSRPPTRARGAPSPGVLGPHASEPQLAPPACTPAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRTR |
| 预测分子量 | 90 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于PANK4(泛酸激酶4)重组蛋白的虚构参考文献示例(内容基于研究领域常见主题虚构，实际文献请通过PubMed/Google Scholar等平台验证)：
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1. **文献名称**: "Recombinant Expression and Biochemical Characterization of Human PANK4 in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究成功在大肠杆菌中表达了重组人源PANK4蛋白，并对其酶动力学特性进行分析。结果表明，重组PANK4具有泛酸磷酸化活性，且其活性受辅酶A负反馈调节，为后续功能研究提供基础工具。
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2. **文献名称**: "Structural Insights into PANK4 Catalytic Mechanism by X-ray Crystallography"
**作者**: Li X, et al.
**摘要**: 通过X射线晶体学解析了重组PANK4蛋白的3D结构，揭示了其ATP结合域和底物识别位点的关键氨基酸残基，阐明了PANK4与其他泛酸激酶亚型的功能差异及进化保守性。
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3. **文献名称**: "PANK4 Knockdown Alters Cellular Coenzyme A Homeostasis and Promotes Apoptosis in Cancer Cells"
**作者**: Garcia R, et al.
**摘要**: 利用重组PANK4蛋白制备抗体，研究其在癌细胞中的功能。结果表明，抑制PANK4表达会导致细胞内辅酶A水平下降，进而诱导线粒体依赖性凋亡，提示PANK4作为潜在癌症治疗靶点。
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**注意**：以上内容为模拟生成，如需真实文献，建议通过以下关键词检索：
`PANK4 recombinant protein`, `pantothenate kinase 4`, `coenzyme A biosynthesis`
推荐数据库：**PubMed** (https://pubmed.ncbi.nlm.nih.gov/) 或 **ScienceDirect** (https://www.sciencedirect.com/)。
**Background of PANK4 Recombinant Protein**
Pantothenate kinase 4 (PANK4) is a member of the pantothenate kinase family, which plays a critical role in regulating coenzyme A (CoA) biosynthesis by catalyzing the phosphorylation of pantothenate (vitamin B5). CoA is an essential cofactor involved in central metabolic pathways, including fatty acid metabolism, the tricarboxylic acid (TCA) cycle, and cellular energy production. Unlike other PANK isoforms (PANK1-3), PANK4 exhibits distinct structural and functional characteristics. It lacks canonical catalytic activity in some species, suggesting potential regulatory or non-canonical roles in CoA metabolism or other cellular processes.
The recombinant PANK4 protein is generated through molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cells), enabling high-purity production for functional studies. Structural analyses reveal conserved domains, such as the catalytic core shared with other PANKs, but unique N-terminal extensions may influence subcellular localization or protein-protein interactions. PANK4 is predominantly expressed in tissues like the liver, brain, and testes, hinting at tissue-specific regulatory functions.
Emerging studies suggest PANK4 may participate in stress responses, autophagy, or apoptosis, though its precise mechanisms remain unclear. Dysregulation of CoA metabolism is linked to neurodegenerative disorders (e.g., PKAN, caused by PANK2 mutations), raising interest in PANK4’s potential compensatory or pathological roles. Recombinant PANK4 facilitates enzymatic assays, inhibitor screening, and interaction studies to explore its biology and therapeutic relevance.
Despite progress, PANK4’s full physiological impact, catalytic potential, and disease associations require further investigation. Its recombinant form remains a vital tool for unraveling these mysteries.
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