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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDGFAA |
| Uniprot No | P04085 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 87-211aa |
| 氨基酸序列 | SIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA TTSLNPDYRE EDTGRPRESG KKRKRKRLKP T |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PDGFAA重组蛋白的参考文献及简要摘要：
1. **《Recombinant PDGF-AA enhances stem cell proliferation and osteogenic differentiation in vitro》**
- **作者**：Smith J, et al.
- **摘要**：研究证明重组PDGF-AA蛋白可显著促进间充质干细胞的增殖，并通过激活PI3K/AKT信号通路增强其成骨分化能力，为骨组织工程提供潜在应用。
2. **《Functional characterization of PDGF-AA in wound healing models》**
- **作者**：Lee H, Kim S.
- **摘要**：通过动物模型发现，局部应用重组PDGF-AA能加速皮肤伤口愈合，机制涉及促进成纤维细胞迁移和胶原沉积，提示其作为慢性溃疡治疗药物的潜力。
3. **《Expression and purification of bioactive human PDGF-AA in E. coli》**
- **作者**：Zhang R, et al.
- **摘要**：报道了一种高效的大肠杆菌表达系统，成功制备高纯度重组人PDGF-AA蛋白，并通过细胞增殖实验验证其生物活性，为大规模生产提供可行方案。
(注：以上文献为虚拟示例，实际引用需查询具体数据库如PubMed或Web of Science。)
Platelet-derived growth factor-AA (PDGF-AA) is a key member of the PDGF family, a group of growth factors involved in cellular regulation and tissue homeostasis. PDGFs function as potent mitogens and chemotactic agents, primarily binding to tyrosine kinase receptors (PDGFR-α and -β). The PDGF family includes five isoforms (PDGF-AA, -AB, -BB, -CC, -DD), with PDGF-AA consisting of two identical A-chain subunits linked by disulfide bonds. It selectively binds to the PDGFR-αα homodimer or PDGFR-αβ heterodimer, activating downstream signaling pathways like MAPK and PI3K/AKT, which drive cell proliferation, migration, and extracellular matrix remodeling.
Recombinant PDGF-AA is produced using biotechnological systems (e.g., mammalian cells or E. coli) to ensure high purity and bioactivity. Its applications span basic research and preclinical studies, particularly in exploring mechanisms of embryogenesis, angiogenesis, wound healing, and mesenchymal cell differentiation. In regenerative medicine, PDGF-AA is investigated for its potential to stimulate tissue repair, though its role in fibrotic disorders and tumor progression (e.g., glioblastoma, stromal activation) necessitates cautious therapeutic evaluation.
Compared to other isoforms, PDGF-AA exhibits unique receptor specificity and functional profiles, making it critical for studying PDGFR-α-driven processes. Commercial recombinant variants are often engineered with tags (e.g., His-tag) for purification and detection. Despite its therapeutic promise, PDGF-AA's dual role in physiological repair and pathological states underscores the need for context-specific research to harness its benefits while mitigating risks.
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