| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTG |
| Uniprot No | P63261 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-375aa |
| 氨基酸序列 | MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ EYDESGPSIV HRKCF |
| 预测分子量 | 41,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTG重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库检索获取)：
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1. **标题**：*"High-yield Expression and Purification of Recombinant Human ACTG1 in Escherichia coli"*
**作者**：Smith A, et al.
**摘要**：本研究报道了人源ACTG1蛋白在大肠杆菌中的高效表达及纯化策略，通过优化密码子使用和诱导条件获得可溶性重组蛋白，并验证其与天然肌动蛋白相似的聚合能力。
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2. **标题**：*"Structural Analysis of ACTG Recombinant Protein Reveals Conserved Actin Filament Dynamics"*
**作者**：Chen L, Wang Y.
**摘要**：利用X射线晶体学解析了重组ACTG蛋白的三维结构，揭示了其与细胞骨架动态调节相关的关键结构域，为研究肌动蛋白相关疾病提供分子基础。
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3. **标题**：*"Functional Characterization of ACTG Mutants in Cancer Cell Migration Using Recombinant Protein Assays"*
**作者**：Johnson R, et al.
**摘要**：通过体外重组ACTG蛋白实验，证明特定突变体可显著影响癌细胞的迁移能力，提示ACTG在肿瘤转移中的潜在作用机制。
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4. **标题**：*"Development of a Mammalian Cell System for Post-translationally Modified ACTG Recombinant Production"*
**作者**：Kim S, et al.
**摘要**：开发基于HEK293细胞的重组ACTG表达系统，成功获得具有天然翻译后修饰(如乙酰化)的功能性蛋白，适用于药物筛选研究。
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**建议**：如需真实文献，可在PubMed或Google Scholar中搜索关键词“recombinant ACTG protein”“ACTG1 expression”或“actin gamma recombinant”。
**Background of ACTG Recombinant Proteins**
ACTG recombinant proteins are engineered variants derived from the actin gamma 1 (ACTG1) gene, which encodes a critical component of the cytoskeleton in eukaryotic cells. Actin proteins, including gamma-actin, are essential for maintaining cell structure, motility, and intracellular signaling. Unlike beta-actin, which is ubiquitously expressed, gamma-actin is predominantly found in cytoskeletal structures of non-muscle cells, playing specialized roles in processes like cell division, migration, and mechanical stability.
The development of recombinant ACTG proteins leverages genetic engineering techniques to produce purified, functional actin in laboratory settings. By cloning the ACTG1 gene into expression vectors (e.g., bacterial, insect, or mammalian systems), researchers generate high-purity gamma-actin with modified features, such as fluorescent tags or affinity tags (e.g., His-tag), to facilitate tracking, purification, or interaction studies. These recombinant proteins retain native folding and functional properties, enabling in vitro studies on actin polymerization, filament dynamics, and interactions with binding partners like profilin or cofilin.
ACTG recombinant proteins are pivotal in studying cytoskeletal disorders, cancer metastasis, and neurodegenerative diseases linked to actin dysregulation. They also serve as tools in drug discovery, particularly for compounds targeting actin dynamics. Challenges in production include maintaining protein solubility and proper post-translational modifications, often addressed through optimized expression systems. Recent advances in cryo-EM and structural biology further utilize recombinant actin to elucidate atomic-level mechanisms of cytoskeletal machinery, underscoring their value in both basic and applied biomedical research.
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