| 纯度 | > 95 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTN1 |
| Uniprot No | P12814-3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSEFMDHYDSQQTNDYMQPEEDWDRDLLL DPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGE RLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLG MIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKD GLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAE DIVGTARPDEKAIMTYVSSFYHAF |
| 预测分子量 | 31 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTN1重组蛋白的3篇代表性文献的简要信息(注：内容基于公开研究领域知识模拟，具体文献需核实)：
1. **标题**：*"Recombinant human ACTN1 regulates cytoskeletal dynamics in megakaryocytes"*
**作者**：Wang Y, et al.
**摘要**：研究利用重组ACTN1蛋白探究其在巨核细胞骨架重塑中的作用，发现其通过调控肌动蛋白交联影响血小板生成，为血小板减少症机制提供新见解。
2. **标题**：*"Structural basis of ACTN1 binding to F-actin revealed by cryo-EM"*
**作者**：Smith J, et al.
**摘要**：通过冷冻电镜解析重组ACTN1蛋白与F-肌动蛋白的复合物结构，阐明其分子结合模式及对细胞运动的关键调控机制。
3. **标题**：*"ACTN1 recombinant protein suppresses tumor metastasis by inhibiting invadopodia formation"*
**作者**：Li X, et al.
**摘要**：体外实验表明重组ACTN1可竞争性抑制癌细胞侵袭伪足形成，降低基质降解能力，提示其作为肿瘤转移治疗的潜在靶点。
(建议通过PubMed或Web of Science以“ACTN1 recombinant protein”为关键词检索获取最新文献)
**Background of ACTN1 Recombinant Protein**
ACTN1 (α-actinin-1) is a cytoskeletal protein belonging to the α-actinin family, which plays critical roles in cross-linking actin filaments and anchoring cytoskeletal structures to cell membranes. It is ubiquitously expressed, particularly in non-muscle cells, and is involved in maintaining cell shape, adhesion, migration, and signal transduction. Structurally, ACTN1 contains an N-terminal actin-binding domain, a central spectrin-repeat rod domain, and a C-terminal calmodulin-like domain that mediates interactions with integrins, signaling molecules, and other cytoskeletal proteins.
The recombinant ACTN1 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian systems, and purified to homogeneity for research applications. Recombinant ACTN1 retains functional properties, enabling studies on its role in cytoskeletal dynamics, cell-matrix interactions, and mechanotransduction. Dysregulation of ACTN1 is linked to pathologies such as cancer metastasis, cardiovascular diseases, and immune disorders, making it a target for therapeutic exploration.
In research, recombinant ACTN1 is utilized to investigate its binding partners, phosphorylation-dependent regulatory mechanisms, and contributions to diseases. It also serves as an antigen for antibody production or a control in diagnostic assays. Recent studies highlight its involvement in platelet activation and thrombus formation, underscoring its biomedical relevance. The availability of recombinant ACTN1 facilitates mechanistic insights into cellular mechanics and disease pathways, supporting drug discovery and biomarker development.
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