| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBd |
| Uniprot No | Q9UJT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MSIVTVQLGQ CGNQIGFEVF DALLSDSHSS QGLCSMRENE AYQASCKERF FSEEENGVPI ARAVLVDMEP KVINQMLSKA AQSGQWKYGQ HACFCQKQGS GNNWAYGYSV HGPRHEESIM NIIRKEVEKC DSFSGFFIIM SMAGGTGSGL GAFVTQNLED QYSNSLKMNQ IIWPYGTGEV IVQNYNSILT LSHLYRSSDA LLLHENDAIH KICAKLMNIK QISFSDINQV LAHQLGSVFQ PTYSAESSFH YRRNPLGDLM EHLVPHPEFK MLSVRNIPHM SENSLAYTTF TWAGLLKHLR QMLISNAKME EGIDRHVWPP LSGLPPLSKM SLNKDLHFNT SIANLVILRG KDVQSADVEG FKDPALYTSW LKPVNAFNVW KTQRAFSKYE KSAVLVSNSQ FLVKPLDMIV GKAWNMFASK AYIHQYTKFG IEEEDFLDSF TSLEQVVASY CNL |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBd(如TUBD1)重组蛋白的示例参考文献(内容为模拟虚构，供参考)：
1. **文献名称**：*"Recombinant TUBD1 Expression and Its Role in Microtubule Dynamics"*
**作者**：Smith A, et al.
**摘要**：研究报道了人源TUBD1重组蛋白在大肠杆菌中的高效表达与纯化，并证明其能促进微管聚合，揭示了TUBD1在细胞分裂中的潜在调控机制。
2. **文献名称**：*"Structural Analysis of TUBD1 Recombinant Protein in Cilia Formation"*
**作者**：Chen L, Wang Y.
**摘要**：通过冷冻电镜解析了TUBD1重组蛋白的三维结构，发现其与纤毛内转运蛋白(IFT)复合物的相互作用，为纤毛相关疾病研究提供结构基础。
3. **文献名称**：*"Functional Characterization of TUBD1 in Cancer Cell Migration"*
**作者**：Garcia R, et al.
**摘要**：利用重组TUBD1蛋白进行体外实验，证明其过表达可抑制肿瘤细胞的迁移能力，提示其作为癌症治疗靶点的可能性。
4. **文献名称**：*"TUBD1 Recombinant Protein as a Biomarker for Primary Ciliary Dyskinesia"*
**作者**：Kim S, et al.
**摘要**：开发了基于重组TUBD1蛋白的抗体检测方法，用于诊断纤毛运动障碍患者的基因突变，提高临床检测灵敏度。
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提示：实际文献需通过PubMed、Google Scholar等平台以关键词“TUBD1 recombinant protein”或“TUBD1 expression”检索，并关注近年发表的细胞生物学或结构研究论文。
**Background of TUBd Recombinant Protein**
TUBd recombinant protein is a engineered form of tubulin, a critical structural component of microtubules in eukaryotic cells. Tubulin exists as heterodimers of α- and β-subunits, which polymerize to form dynamic microtubule networks essential for cellular processes like mitosis, intracellular transport, and cell motility. The "TUBd" designation likely refers to a specific isoform, mutant, or truncated variant (e.g., δ-tubulin or a domain-specific construct) designed for targeted functional studies or therapeutic applications.
Recombinant TUBd is typically produced using heterologous expression systems, such as *E. coli* or insect cells, enabling high-yield purification and post-translational modifications if required. Its production allows researchers to study tubulin interactions, microtubule dynamics, and the effects of mutations or drug binding in vitro. For instance, TUBd may be utilized to investigate mechanisms of microtubule-targeting agents (e.g., taxanes or vinca alkaloids) used in cancer therapy, or to explore genetic disorders linked to tubulin dysfunction, such as neurodevelopmental syndromes.
Additionally, recombinant TUBd serves as a tool for structural biology (e.g., cryo-EM or X-ray crystallography) to resolve molecular details of tubulin folding, dimerization, or binding interfaces. In drug discovery, it facilitates high-throughput screening for novel compounds modulating microtubule stability. Its application extends to diagnostics, where it may aid in detecting autoantibodies in autoimmune diseases or serve as a biomarker in certain cancers.
Overall, TUBd recombinant protein bridges fundamental research and translational studies, offering insights into cytoskeletal biology and therapeutic innovation.
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