| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACY1 |
| Uniprot No | Q03154 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-408aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMTSKGPEEEHPSVTLFRQYLRIRTVQPKPD YGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPGTNPTLSSILLNSH TDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRRLKV EGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANP TDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFR EKEWQRLQSNPHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPD VDFKAFEEQLQSWCQAAGEGVTLEFAQKWMHPQVTPTDDSNPWWAAFSRV CKDMNLTLEPEIMPAATDNRYIRAVGVPALGFSPMNRTPVLLHDHDERLH EAVFLRGVDIYTRLLPALASVPALPSDS |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACY1重组蛋白的3篇文献摘要信息(示例仅供参考，具体文献需根据实际数据库检索补充)：
1. **文献名称**: *"Expression and purification of recombinant human aminoacylase-1 (ACY1) in Escherichia coli for enzymatic characterization"*
**作者**: Müller, P. et al.
**摘要**: 研究报道了人源ACY1在大肠杆菌中的重组表达及纯化方法，优化了可溶性蛋白产量，并验证其水解乙酰化氨基酸的酶活性，为后续功能研究提供基础。
2. **文献名称**: *"Crystal structure of recombinant mouse aminoacylase-1: insights into substrate specificity and catalytic mechanism"*
**作者**: Tanaka, K. et al.
**摘要**: 通过X射线晶体学解析小鼠ACY1重组蛋白的三维结构，揭示了其底物结合口袋的关键氨基酸残基，并探讨了金属离子在催化反应中的作用机制。
3. **文献名称**: *"Role of recombinant ACY1 in modulating cellular oxidative stress in a zebrafish model"*
**作者**: Li, X. et al.
**摘要**: 利用重组ACY1蛋白处理斑马鱼模型，证明其通过调节谷胱甘肽代谢通路减轻氧化应激损伤，提示其在代谢疾病中的潜在治疗价值。
(注：以上文献为模拟示例，实际引用请通过PubMed/Web of Science等平台检索最新论文。)
**Background of ACY1 Recombinant Protein**
Aminoacylase-1 (ACY1) is a zinc-binding enzyme belonging to the M20 metalloprotease family, encoded by the *ACY1* gene located on human chromosome 3p21.1. It plays a critical role in cellular metabolism by catalyzing the hydrolysis of N-acetylated amino acids into free amino acids and acetic acid, facilitating amino acid recycling and detoxification. This reaction is vital for maintaining metabolic homeostasis, particularly in the kidneys, liver, and brain.
ACY1 deficiency, a rare autosomal recessive disorder, leads to urinary excretion of specific N-acetylated metabolites and is associated with neurological impairments, highlighting its importance in neural function. Recombinant ACY1 protein, produced via heterologous expression systems like *E. coli* or mammalian cell lines, enables detailed study of its structure, enzymatic mechanisms, and pathological role. Structurally, ACY1 functions as a homodimer with conserved zinc-binding motifs critical for catalytic activity.
Research on recombinant ACY1 has expanded into disease biomarker discovery, particularly in cancer, where altered ACY1 expression correlates with tumor progression. It is also explored for therapeutic applications, such as enzyme replacement therapy for genetic deficiencies and as a tool for drug screening. Its recombinant form retains native enzymatic properties, making it valuable for industrial biocatalysis and diagnostic assay development.
Overall, ACY1 recombinant protein serves as a key resource for unraveling metabolic pathways, understanding genetic disorders, and advancing translational research in precision medicine.
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