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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPA |
| Uniprot No | Q8LPQ9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-901aa |
| 氨基酸序列 | MALSMKPFRADDSGFQSNNLWVGSLTPETTESDLTELFGRYGDIDRITVYSSRGFAFIYYRHVEEAVAAKEALQGANLNGSQIKIEYARPAKPCKSLWVGGIGPNVSKDDLEEEFSKFGKIEDFRFLRERKTAFIDYYEMDDALQAKSMNGKPMGGSFLRVDFLRSQAPKKEQWAGSYDNRNGNMNHKPQYPHSYEDFKGDVQPSKVLWIGFPPTATQCNDEQILHNAMILFGEIERVKSYPSRNFALVEFRSAEEARQCKEGLQGRLFNNPRIKIMYSNDELPPEQDDTSFYSGMKRSRTDMFNNDPSFVSSPHSTGIPGSMRPLRGTNERSYNGAEYNDVVGKEPNWRRPSANGTGILPSPTGPGILPSPAQGTRRPMRSNPDSWEGYDPAQLVRESKRTRRDGSVDGFTPMGVDERSFGRGSVAARPIRGPPDSDHIWRGMIAKGGTPVCCARCVPMGKGIETKLPEVVNCSARTDLNMLAKHYAVAIGCEIVFFVPDREEDFASYTEFLRYLSSKDRAGVAKLDDGTTLFLVPPSDFLTDVLQVTRQERLYGVVLKLPPPAVPVTASYRQESQSNPLHYMDQARDSPANASHSLYPPRENYIRGAPEHLTAASKPSVSEPLRIPNNAAPQAGVSLTPELLATLASILPATSQPAAPESHQPMSGPSTVVSTAHQSNGLYNGEAPSQAWKRGPQTVHDASNQSFQQYGNQYTPAGQLPPPPSRYPPASNNPNYTSGMVHGNMQYQSQSVNMPQLSPLPNMPHNNYSMYTQGSSNHPVSQPMVQQYQPEASMPNQNYGPIPSYQQANFHGVTTNQAQNLNPSQFQAAMQPPADKANLEPQNQALRLQPMISGDGQGTTDGEVDKNQRYQSTLQFAANLLLQIQQKQQQQSSGTPAGQGP |
| 预测分子量 | 99,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FPA重组蛋白的3篇参考文献示例(注：以下内容为模拟虚构，仅供参考)：
1. **文献名称**：*Recombinant FPA as a Serological Marker for Malaria Diagnosis*
**作者**：Smith J. et al.
**摘要**：研究开发了一种基于重组FPA蛋白的血清学检测方法，用于疟疾感染的快速诊断。实验表明，该重组蛋白与患者血清中抗体具有高特异性结合，为资源有限地区的疟疾筛查提供了新工具。
2. **文献名称**：*Structural and Functional Analysis of FPA Recombinant Protein in Hemostasis*
**作者**：Lee H. et al.
**摘要**：通过X射线晶体学解析了重组FPA蛋白的三维结构，并验证其在凝血级联反应中的功能。研究发现，重组FPA能有效促进血小板聚集，为血友病治疗药物的开发提供了理论依据。
3. **文献名称**：*FPA-Based Recombinant Vaccine Induces Protective Immunity Against Tumor Growth*
**作者**：Wang Y. et al.
**摘要**：利用重组FPA蛋白作为肿瘤抗原载体，在小鼠模型中成功诱导了特异性T细胞免疫应答，显著抑制了黑色素瘤的生长。研究表明，FPA重组蛋白在癌症免疫治疗中具有潜在应用价值。
4. **文献名称**：*Expression and Purification of Recombinant FPA for Biotechnological Applications*
**作者**：Garcia R. et al.
**摘要**：优化了重组FPA蛋白在大肠杆菌中的表达和纯化工艺，获得高纯度、高活性的产物。该研究为大规模生产FPA重组蛋白用于工业或医疗用途提供了技术方案。
(注：以上文献信息为模拟生成，实际研究中请通过学术数据库检索具体文献。)
**Background of FPA Recombinant Protein**
FPA (Fibrinopeptide A) recombinant protein is a biologically engineered molecule derived from fibrinogen, a critical glycoprotein involved in blood coagulation. During the clotting cascade, thrombin cleaves fibrinogen to release fibrinopeptide A (FPA), enabling fibrin monomer polymerization and clot formation. Recombinant FPA is synthesized using genetic engineering techniques, typically by inserting the FPA-encoding gene into expression systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of pure, standardized FPA for research and therapeutic applications.
FPA plays a pivotal role in studying thrombosis, hemostasis, and fibrinolysis. Its recombinant form is particularly valuable in biomedical research to investigate clot structure, dynamics, and interactions with anticoagulant drugs. Additionally, it serves as a biomarker in clinical diagnostics; elevated FPA levels in blood correlate with hypercoagulable states, such as deep vein thrombosis or disseminated intravascular coagulation (DIC).
The development of recombinant FPA addresses challenges associated with isolating the peptide from natural sources, which is labor-intensive and yields low quantities. Engineered FPA ensures consistency, reduces batch variability, and eliminates contamination risks from plasma-derived products. Recent advancements in protein engineering have further optimized its stability and bioactivity, enhancing its utility in *in vitro* assays, drug screening, and antibody production for diagnostic kits.
Overall, FPA recombinant protein bridges gaps in understanding coagulation mechanisms and supports innovations in diagnosing and treating clotting disorders, underscoring its importance in both basic science and clinical practice.
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