| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP5b |
| Uniprot No | P06576 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 48-529aa |
| 氨基酸序列 | AQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS |
| 预测分子量 | 53.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATP5b重组蛋白的3篇参考文献示例(文献信息为示例，实际引用时请核实真实来源)：
1. **文献名称**：Expression and Functional Characterization of Recombinant ATP5b in Escherichia coli
**作者**：Smith A, et al.
**摘要**：该研究报道了在大肠杆菌中成功表达并纯化ATP5b重组蛋白，通过体外酶活实验证实其具有ATP合酶活性，为线粒体能量代谢研究提供工具。
2. **文献名称**：ATP5b Recombinant Protein Promotes Mitochondrial Function Recovery in Cellular Models
**作者**：Zhang Y, et al.
**摘要**：利用HEK293细胞表达ATP5b重组蛋白，证明其可修复缺陷细胞的线粒体膜电位并增强ATP生成，提示其在代谢疾病治疗中的潜在应用。
3. **文献名称**：Cryo-EM Structure of Recombinant ATP5b Reveals Conformational Changes During ATP Synthesis
**作者**：Johnson R, et al.
**摘要**：通过冷冻电镜解析重组ATP5b的高分辨率结构，揭示其在不同催化状态下的构象变化，阐明了ATP合酶的工作机制。
(注：以上文献为示例性质，建议通过PubMed或Web of Science等数据库检索真实文献。)
ATP5b, also known as ATP synthase subunit beta, is a critical component of mitochondrial ATP synthase (Complex V), the enzyme responsible for generating adenosine triphosphate (ATP) during oxidative phosphorylation. Located in the mitochondrial inner membrane, ATP synthase catalyzes the final step of cellular respiration by converting ADP to ATP using energy from the proton gradient. The beta subunit (ATP5b) plays a central role in this process, forming the catalytic core that binds ADP and inorganic phosphate to synthesize ATP. Its structural and functional conservation across eukaryotes underscores its essentiality in energy metabolism.
Recombinant ATP5b protein is engineered using genetic cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., HEK293 cells) to ensure proper folding and post-translational modifications. This recombinant form retains enzymatic activity and structural integrity, enabling studies on mitochondrial function, ATP synthesis mechanisms, and diseases linked to ATP synthase dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer. Researchers use it to investigate mutations affecting ATP5b, which may impair energy production and contribute to pathologies like Leigh syndrome or cardiomyopathy.
Additionally, ATP5b has emerged as a potential biomarker or therapeutic target. In cancer, altered ATP synthase expression correlates with tumor progression and drug resistance. Autoantibodies against ATP5b are also detected in autoimmune conditions, highlighting its immunogenic role. The availability of recombinant ATP5b facilitates drug screening, antibody development, and structural studies using techniques like cryo-EM. By elucidating ATP synthase regulation and dysfunction, this tool advances our understanding of cellular energetics and disease mechanisms, bridging basic research with translational applications.
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