| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HIP1R |
| Uniprot No | O75146 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1068aa |
| 氨基酸序列 | MNSIKNVPARVLSRRPGHSLEAEREQFDKTQAISISKAINTQEAPVKEKH ARRIILGTHHEKGAFTFWSYAIGLPLPSSSILSWKFCHVLHKVLRDGHPN VLHDCQRYRSNIREIGDLWGHLHDRYGQLVNVYTKLLLTKISFHLKHPQF PAGLEVTDEVLEKAAGTDVNNIFQLTVEMFDYMDCELKLSESVFRQLNTA IAVSQMSSGQCRLAPLIQVIQDCSHLYHYTVKLLFKLHSCLPADTLQGHR DRFHEQFHSLRNFFRRASDMLYFKRLIQIPRLPEGPPNFLRASALAEHIK PVVVIPEEAPEDEEPENLIEISTGPPAGEPVVVADLFDQTFGPPNGSVKD DRDLQIESLKREVEMLRSELEKIKLEAQRYIAQLKSQVNALEGELEEQRK QKQKALVDNEQLRHELAQLRAAQLEGERSQGLREEAERKASATEARYNKL KEKHSELVHVHAELLRKNADTAKQLTVTQQSQEEVARVKEQLAFQVEQVK RESELKLEEKSDQLEKLKRELEAKAGELARAQEALSHTEQSKSELSSRLD TLSAEKDALSGAVRQREADLLAAQSLVRETEAALSREQQRSSQEQGELQG RLAERESQEQGLRQRLLDEQFAVLRGAAAEAAGILQDAVSKLDDPLHLRC TSSPDYLVSRAQEALDAVSTLEEGHAQYLTSLADASALVAALTRFSHLAA DTIINGGATSHLAPTDPADRLIDTCRECGARALELMGQLQDQQALRHMQA SLVRTPLQGILQLGQELKPKSLDVRQEELGAVVDKEMAATSAAIEDAVRR IEDMMNQARHASSGVKLEVNERILNSCTDLMKAIRLLVTTSTSLQKEIVE SGRGAATQQEFYAKNSRWTEGLISASKAVGWGATQLVEAADKVVLHTGKY EELIVCSHEIAASTAQLVAASKVKANKHSPHLSRLQECSRTVNERAANVV ASTKSGQEQIEDRDTMDFSGLSLIKLKKQEMETQVRVLELEKTLEAERMR LGELRKQHYVLAGASGSPGEEVAIRPSTAPRSVTTKKPPLAQKPSVAPRQ DHQLDKKDGIYPAQLVNY |
| 预测分子量 | 146 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HIP1R重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**：*HIP1R regulates clathrin-mediated endocytosis and actin dynamics in vesicle formation*
**作者**：Engqvist-Goldstein ÅE, et al.
**摘要**：研究揭示了HIP1R重组蛋白通过结合网格蛋白(clathrin)和肌动蛋白(actin)，调控内吞小泡的形成。实验表明，HIP1R通过其C端结构域稳定网格蛋白包被的囊泡，并协调肌动蛋白骨架重塑以促进膜内陷。
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2. **文献名称**：*HIP1R promotes tumor metastasis by modulating cytoskeletal reorganization in cancer cells*
**作者**：Zhao X, et al.
**摘要**：该文献发现HIP1R重组蛋白在多种癌细胞中高表达，通过激活Rho GTPase信号通路驱动细胞迁移和侵袭。体外实验显示，HIP1R缺失显著抑制肌动蛋白纤维的聚合，从而降低肿瘤转移能力。
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3. **文献名称**：*Structural and functional characterization of recombinant HIP1R in Huntington's disease models*
**作者**：Li SH, Li XJ.
**摘要**：研究利用重组HIP1R蛋白分析其与突变亨廷顿蛋白(mHTT)的相互作用。结果显示，HIP1R通过N端结构域结合mHTT，可能参与神经元内吞功能障碍，为亨廷顿病的病理机制提供了新见解。
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如果需要更具体的研究方向或补充文献，可进一步说明!
**Background of HIP1R Recombinant Protein**
Huntingtin-interacting protein 1-related protein (HIP1R), also known as HIP1R or HIP12. is a multidomain adaptor protein involved in membrane trafficking and cytoskeletal organization. It belongs to the Sla2/HIP1 family, sharing structural and functional homology with yeast Sla2p. HIP1R interacts with clathrin, actin, and endocytic machinery, serving as a bridge between the cell membrane and cytoskeleton during clathrin-mediated endocytosis (CME). Its N-terminal ANTH domain binds phosphatidylinositol lipids, anchoring it to membranes, while the C-terminal talin-like domain associates with actin filaments.
HIP1R regulates vesicle formation by coordinating membrane curvature and actin polymerization. It also stabilizes clathrin-coated pits and promotes receptor internalization, such as EGFR, impacting signal transduction. Dysregulation of HIP1R is linked to neurodegenerative diseases, notably Huntington’s disease (HD), as it interacts with mutant huntingtin protein, altering endocytosis and synaptic function. Additionally, HIP1R overexpression in cancers correlates with cell migration and metastasis.
Recombinant HIP1R proteins are engineered for *in vitro* studies to dissect molecular interactions, structural dynamics, and functional roles in disease pathways. Produced via bacterial or mammalian expression systems, these purified proteins retain functional domains for binding assays, enzymatic studies, or drug screening. Research using HIP1R recombinant proteins enhances understanding of endocytic-cytoskeletal crosstalk and offers therapeutic insights for HD, cancer, and trafficking-related disorders.
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