| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PICP |
| Uniprot No | P42790 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 氨基酸序列 | MKSSAAKQTVLCLNRYAVVALPLAIASFAAFGASPASTLWAPTDTKAFVTPAQVEARSAAPLLELAAGETAHIVVSLKLRDEAQLKQLAQAVNQPGNAQFGKFLKRRQFLSQFAPTEAQVQAVVAHLRKNGFVNIHVVPNRLLISADGSAGAVKAAFNTPLVRYQLNGKAGYANTAPAQVPQDLGEIVGSVLGLQNVTRAHPMLKVGERSAAKTLAAGTAKGHNPTEFPTIYDASSAPTAANTTVGIITIGGVSQTLQDLQQFTSANGLASVNTQTIQTGSSNGDYSDDQQGQGEWDLDSQSIVGSAGGAVQQLLFYMADQSASGNTGLTQAFNQAVSDNVAKVINVSLGWCEADANADGTLQAEDRIFATAAAQGQTFSVSSGDEGVYECNNRGYPDGSTYSVSWPASSPNVIAVGGTTLYTTSAGAYSNETVWNEGLDSNGKLWATGGGYSVYESKPSWQSVVSGTPGRRLLPDISFDAAQGTGALIYNYGQLQQIGGTSLASPIFVGLWARLQSANSNSLGFPAASFYSAISSTPSLVHDVKSGNNGYGGYGYNAGTGWDYPTGWGSLDIAKLSAYIRSNGFGH |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PICP重组蛋白的示例参考文献(请注意，以下内容为模拟生成的示例，非真实文献)：
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1. **文献名称**: *Expression and Purification of Recombinant Procollagen Type I C-Terminal Propeptide (PICP) in Escherichia coli*
**作者**: Zhang Y, et al.
**摘要**: 本研究报道了在大肠杆菌系统中高效表达重组PICP蛋白的优化策略，包括密码子优化和诱导条件调整。通过亲和层析纯化获得高纯度蛋白，并验证其与天然PICP的免疫反应性，为肝纤维化诊断试剂的开发奠定了基础。
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2. **文献名称**: *Functional Characterization of Recombinant PICP in Osteoblast Differentiation*
**作者**: Lee S, et al.
**摘要**: 作者利用哺乳动物细胞表达系统制备重组PICP，证明其通过激活TGF-β信号通路促进成骨细胞分化和骨形成，为骨代谢疾病(如骨质疏松)的治疗提供了潜在分子靶点。
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3. **文献名称**: *Recombinant PICP as a Therapeutic Agent in Pulmonary Fibrosis Models*
**作者**: Gupta R, et al.
**摘要**: 该研究在小鼠肺纤维化模型中评估重组PICP的治疗效果，发现其通过抑制过度胶原沉积减轻纤维化病理，提示重组PICP在抗纤维化药物开发中的应用潜力。
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4. **文献名称**: *Structural Insights into Recombinant PICP by X-ray Crystallography*
**作者**: Müller J, et al.
**摘要**: 通过X射线晶体学解析了重组PICP的三维结构，揭示了其与胶原前体结合的分子机制，为设计靶向胶原合成异常的抑制剂提供了结构基础。
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**说明**：以上文献为示例，实际研究中建议通过PubMed、Web of Science等数据库检索最新文献，并关注期刊如*Biomaterials*、*Journal of Molecular Biology*等。
**Background of PICP Recombinant Protein**
Procollagen I C-terminal propeptide (PICP) is a biologically active peptide cleaved during the synthesis of type I collagen, the most abundant structural protein in vertebrates. As a byproduct of collagen maturation, PICP serves as a biomarker for assessing collagen formation and tissue remodeling processes. Elevated or reduced PICP levels are associated with fibrotic disorders (e.g., liver cirrhosis, pulmonary fibrosis), bone metabolism abnormalities (e.g., osteoporosis), and connective tissue diseases, making it clinically significant in diagnostics and therapeutic monitoring.
Recombinant PICP is engineered using biotechnological methods to produce a standardized, high-purity form of the protein. This involves cloning the PICP-encoding gene sequence into expression vectors (e.g., bacterial, mammalian, or insect cell systems) followed by purification via chromatography. Recombinant technology ensures batch-to-batch consistency, scalability, and reduced immunogenicity compared to animal-derived sources.
In research, recombinant PICP is widely used as a calibration standard in immunoassays (e.g., ELISA) to quantify endogenous PICP in clinical samples. It also aids in studying collagen biosynthesis pathways, evaluating antifibrotic drugs, and exploring mechanisms in wound healing or tissue regeneration. Additionally, recombinant PICP supports the development of targeted therapies, such as monoclonal antibodies or inhibitors, to modulate collagen deposition in fibrotic diseases. Its role in bone health studies, particularly in osteoporosis drug development, further underscores its versatility as a tool in both basic and applied biomedical research.
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