| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OVGP1 |
| Uniprot No | Q12889 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-678aa |
| 氨基酸序列 | HKLVCYFTN WAHSRPGPAS ILPHDLDPFL CTHLIFAFAS MNNNQIVAKD LQDEKILYPE FNKLKERNRE LKTLLSIGGW NFGTSRFTTM LSTFANREKF IASVISLLRT HDFDGLDLFF LYPGLRGSPM HDRWTFLFLI EELLFAFRKE ALLTMRPRLL LSAAVSGVPH IVQTSYDVRF LGRLLDFINV LSYDLHGSWE RFTGHNSPLF SLPEDPKSSA YAMNYWRKLG APSEKLIMGI PTYGRTFRLL KASKNGLQAR AIGPASPGKY TKQEGFLAYF EICSFVWGAK KHWIDYQYVP YANKGKEWVG YDNAISFSYK AWFIRREHFG GAMVWTLDMD DVRGTFCGTG PFPLVYVLND ILVRAEFSST SLPQFWLSSA VNSSSTDPER LAVTTAWTTD SKILPPGGEA GVTEIHGKCE NMTITPRGTT VTPTKETVSL GKHTVALGEK TEITGAMTMT SVGHQSMTPG EKALTPVGHQ SVTTGQKTLT SVGYQSVTPG EKTLTPVGHQ SVTPVSHQSV SPGGTTMTPV HFQTETLRQN TVAPRRKAVA REKVTVPSRN ISVTPEGQTM PLRGENLTSE VGTHPRMGNL GLQMEAENRM MLSSSPVIQL PEQTPLAFDN RFVPIYGNHS SVNSVTPQTS PLSLKKEIPE NSAVDEEA |
| 预测分子量 | 75,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OVGP1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Recombinant OVGP1 enhances sperm capacitation and fertilization in vitro*
**作者**: Léveillé, M.C., et al.
**摘要**: 研究重组OVGP1蛋白在体外受精中的作用，发现其通过调控精子获能相关信号通路，提高精子穿透卵母细胞的能力，并改善受精率。
2. **文献名称**: *Role of recombinant oviductal glycoprotein 1 in early embryo development*
**作者**: Abe, H., et al.
**摘要**: 探讨重组OVGP1对牛胚胎体外发育的影响，证实其作为培养基添加剂可显著提升囊胚形成率，并促进胚胎细胞增殖和代谢活性。
3. **文献名称**: *Structural and functional characterization of recombinant human OVGP1*
**作者**: Sendai, Y., et al.
**摘要**: 通过哺乳动物细胞系统表达并纯化人源重组OVGP1.解析其糖基化修饰模式及与透明带蛋白的相互作用，揭示其在保护胚胎免受氧化应激中的分子机制。
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以上文献聚焦于重组OVGP1在生殖生物学中的功能，涵盖受精促进、胚胎发育优化及分子机制解析等领域。如需具体文献来源，建议通过PubMed或Google Scholar按标题及作者检索。
OVGP1 (Oviductal Glycoprotein 1), also known as oviduct-specific glycoprotein or mucin-9. is a high-molecular-weight glycoprotein secreted by the epithelial cells of the mammalian oviduct (fallopian tube in humans). It plays critical roles in reproductive biology, particularly during fertilization and early embryonic development. OVGP1 interacts with gametes and embryos, influencing sperm capacitation, zona pellucida stability, and embryo quality. Its conserved structure across species, characterized by tandem repeat domains and extensive glycosylation, facilitates its function in modulating the oviductal microenvironment.
Recombinant OVGP1 protein is produced using biotechnological platforms (e.g., mammalian expression systems like HEK293 or CHO cells) to overcome challenges in isolating native protein from biological fluids. This engineered form retains functional domains while ensuring batch-to-batch consistency, high purity, and scalability. Studies using recombinant OVGP1 have clarified its molecular mechanisms, revealing its ability to bind sperm surface proteins, prevent polyspermy, and support embryo-maternal communication through growth factor-like domains.
Research applications span in vitro fertilization (IVF) optimization, embryo culture media supplementation, and modeling oviductal physiology. Clinically, it holds potential as a biomarker for oviduct-related infertility or a therapeutic agent to improve assisted reproductive technologies. Recent advances also explore its roles beyond reproduction, including immunomodulation and cancer biology. However, species-specific functional variations necessitate careful interpretation of experimental models. Ongoing studies aim to standardize recombinant OVGP1 formulations and validate their efficacy in translational settings.
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