| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOVA1 |
| Uniprot No | P51513 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-507aa |
| 氨基酸序列 | MAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGTTERVCLIQGTVEALNAVHGFIAEKIREMPQNVAKTEPVSILQPQTTVNPDRIKQTLPSSPTTTKSSPSDPMTTSRANQVKIIVPNSTAGLIIGKGGATVKAVMEQSGAWVQLSQKPDGINLQERVVTVSGEPEQNRKAVELIIQKIQEDPQSGSCLNISYANVTGPVANSNPTGSPYANTAEVLPTAAAAAGLLGHANLAGVAAFPAVLSGFTGNDLVAITSALNTLASYGYNLNTLGLGLSQAAATGALAAAAASANPAAAAANLLATYASEASASGSTAGGTAGTFALGSLAAATAATNGYFGAASPLAASAILGTEKSTDGSKDVVEIAVPENLVGAILGKGGKTLVEYQELTGARIQISKKGEFVPGTRNRKVTITGTPAATQAAQYLITQRITYEQGVRAANPQKVG |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOVA1重组蛋白的3篇参考文献示例(注：文献信息为虚拟模拟，仅作格式参考)：
1. **标题**：*Expression and functional characterization of recombinant NOVA1 in RNA splicing regulation*
**作者**：Chen L, et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了NOVA1重组蛋白，通过体外RNA结合实验证实其特异性结合靶mRNA的YCAY基序，并调控神经细胞选择性剪接活性。
2. **标题**：*Structural analysis of NOVA1 protein domains using recombinant expression in mammalian cells*
**作者**：Kim S, et al.
**摘要**：利用哺乳动物细胞系统表达NOVA1重组蛋白，通过X射线晶体学解析其KH结构域的三维结构，揭示了其与RNA相互作用的分子机制。
3. **标题**：*NOVA1 recombinant protein rescues synaptic defects in neuronal knockout models*
**作者**：Wang Y, et al.
**摘要**：通过体外神经元培养实验，证明外源性重组NOVA1蛋白可恢复NOVA1敲除模型的突触形成障碍，提示其在神经发育中的治疗潜力。
(若需真实文献，建议在PubMed或Google Scholar中检索关键词“NOVA1 recombinant protein”获取具体信息。)
NOVA1 (Neuro-oncological ventral antigen 1) is a RNA-binding protein primarily known for its role in regulating alternative splicing in neuronal cells. It belongs to the NOVA family of proteins, which are critical for neural development and function. NOVA1 is highly expressed in the central nervous system, particularly in regions associated with motor control and synaptic plasticity. It interacts with specific RNA motifs, influencing the inclusion or exclusion of exons during mRNA processing, thereby diversifying protein isoforms essential for proper neuronal connectivity and signaling.
Research has linked NOVA1 dysfunction to neurological disorders, including paraneoplastic opsoclonus-myoclonus ataxia (POMA) and certain forms of epilepsy. Its involvement in post-transcriptional regulation also extends to neurodevelopmental processes, such as axon guidance and synapse formation. Notably, NOVA1 knockout studies in mice result in lethal motor deficits, underscoring its non-redundant role in neural networks.
Recombinant NOVA1 protein is produced using recombinant DNA technology, typically in bacterial or mammalian expression systems. This allows large-scale purification of the protein for structural studies (e.g., crystallography), in vitro RNA-binding assays, or functional investigations into spliceosome dynamics. Researchers utilize it to map RNA interaction sites, characterize splicing mechanisms, and explore therapeutic strategies targeting RNA-protein interactions in neurological diseases. Recent interest has also emerged in its potential roles beyond the nervous system, including cancer biology, where aberrant splicing may contribute to tumor progression.
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