| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | APPBP1 |
| Uniprot No | Q13564 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-534aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAQLGKLLKEQKYDRQLRLWGDHGQEA LESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQ RSSIGKNRAEAAMEFLQELNSDVSGSFVEESPENLLDNDPSFFCRFTVVV ATQLPESTSLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPD NALEDLRLDKPFPELREHFQSYDLDHMEKKDHSHTPWIVIIAKYLAQWYS ETNGRIPKTYKEKEDFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALN TTQIPSSIEDIFNDDRCINITKQTPSFWILARALKEFVAKEGQGNLPVRG TIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISE KELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEIISSMDNPDNEIVLYL MLRAVDRFHKQQGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDD YVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQ TSATFQL |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于APPBP1重组蛋白的3篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Structural Insights into the APPBP1-UBA3-NEDD8-ATP Complex*
**作者**：Gong L, et al.
**摘要**：该研究通过X射线晶体学解析了APPBP1与UBA3、NEDD8及ATP形成的复合物三维结构，揭示了APPBP1在NEDD8激活酶(NAE)中的关键作用，为靶向NAE的药物设计提供了结构基础。
2. **文献名称**：*Functional Characterization of Recombinant APPBP1 in Ubiquitin-Like Protein Conjugation*
**作者**：Chen Z, et al.
**摘要**：文章利用重组表达的APPBP1蛋白，证明其与UBA3协同催化NEDD8与靶蛋白的共价结合，并发现APPBP1的缺失会显著抑制细胞周期进程，提示其在泛素化类似通路中的核心功能。
3. **文献名称**：*APPBP1 Interacts with Amyloid Precursor Protein and Modulates Aβ Production*
**作者**：Wang Y, et al.
**摘要**：该研究通过免疫共沉淀和体外结合实验，证实重组APPBP1直接结合APP(淀粉样前体蛋白)，调控其加工过程并影响β-淀粉样蛋白(Aβ)的生成，暗示APPBP1在阿尔茨海默病病理中的潜在作用。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“APPBP1 recombinant protein”“NAE complex”“NEDD8 activation”等。
APPBP1 (Amyloid Beta Precursor Protein Binding Protein 1), also known as BARA1 or TSG118. is a multifunctional adaptor protein initially identified for its interaction with the amyloid-beta precursor protein (APP). This interaction links APPBP1 to pathways implicated in neuronal development, intracellular signaling, and potentially Alzheimer’s disease pathology. Structurally, APPBP1 contains a conserved ubiquitin-like (UBL) domain and a tetratricopeptide repeat (TPR) motif, facilitating protein-protein interactions. It serves as a regulatory subunit of the NEDD8-activating enzyme (NAE) complex, essential for the neddylation pathway—a post-translational modification process critical for protein stability, localization, and activity, particularly in cell cycle regulation and stress responses.
Recombinant APPBP1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and interactions. Its recombinant form enables researchers to explore molecular mechanisms underlying APP processing, neddylation-dependent ubiquitination cascades, and crosstalk with pathways like the PI3K/AKT/mTOR network. Dysregulation of APPBP1 has been associated with cancer progression, neurodevelopmental disorders, and neurodegeneration. For example, overexpression in certain cancers correlates with enhanced cell proliferation and chemoresistance, while reduced levels may impair synaptic function in neurons.
In drug discovery, recombinant APPBP1 is utilized to screen inhibitors targeting the NAE complex, with potential therapeutic applications in malignancies. Additionally, it aids in structural studies to map binding interfaces with APP or other partners, offering insights into disease mechanisms. Overall, APPBP1 recombinant protein serves as a vital tool for dissecting its roles in cellular homeostasis and disease, bridging basic research to translational applications.
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