| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CADPS |
| Uniprot No | Q9ULU8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1353aa |
| 氨基酸序列 | MLDPSSSEEESDEIVEEESGKEVLGSAPSGARLSPSRTSEGSAGSAGLGGGGAGAGAGVGAGGGGGSGASSGGGAGGLQPSSRAGGGRPSSPSPSVVSEKEKEELERLQKEEEERKKRLQLYVFVMRCIAYPFNAKQPTDMARRQQKISKQQLQTVKDRFQAFLNGETQIMADEAFMNAVQSYYEVFLKSDRVARMVQSGGCSANDSREVFKKHIEKRVRSLPEIDGLSKETVLSSWMAKFDAIYRGEEDPRKQQARMTASAASELILSKEQLYEMFQNILGIKKFEHQLLYNACQLDNPDEQAAQIRRELDGRLQMADQIARERKFPKFVSKEMENMYIEELKSSVNLLMANLESMPVSKGGEFKLQKLKRSHNASIIDMGEESENQLSKSDVVLSFSLEVVIMEVQGLKSLAPNRIVYCTMEVEGGEKLQTDQAEASKPTWGTQGDFSTTHALPAVKVKLFTESTGVLALEDKELGRVILHPTPNSPKQSEWHKMTVSKNCPDQDLKIKLAVRMDKPQNMKHSGYLWAIGKNVWKRWKKRFFVLVQVSQYTFAMCSYREKKAEPQELLQLDGYTVDYTDPQPGLEGGRAFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSHKPVPPTQVQKLNAKGGNVPQLDAPISQFYADRAQKHGMDEFISSNPCNFDHASLFEMVQRLTLDHRLNDSYSCLGWFSPGQVFVLDEYCARNGVRGCHRHLCYLRDLLERAENGAMIDPTLLHYSFAFCASHVHGNRPDGIGTVTVEEKERFEEIKERLRVLLENQITHFRYCFPFGRPEGALKATLSLLERVLMKDIVTPVPQEEVKTVIRKCLEQAALVNYSRLSEYAKIEENQKDAENVGRLITPAKKLEDTIRLAELVIEVLQQNEEHHAEPHVDKGEAFAWWSDLMVEHAETFLSLFAVDMDAALEVQPPDTWDSFPLFQLLNDFLRTDYNLCNGKFHKHLQDLFAPLVVRYVDLMESSIAQSIHRGFERESWEPVKSLTSNLPNVNLPNVNLPKVPNLPVNIPLGIPQMPTFSAPSWMAAIYDADNGSGTSEDLFWKLDALQTFIRDLHWPEEEFGKHLEQRLKLMASDMIESCVKRTRIAFEVKLQKTSRSTDFRVPQSICTMFNVMVDAKAQSTKLCSMEMGQEHQYHSKIDELIEETVKEMITLLVAKFVTILEGVLAKLSRYDEGTLFSSFLSFTVKAASKYVDVPKPGMDVADAYVTFVRHSQDVLRDKVNEEMYIERLFDQWYNSSMNVICTWLTDRMDLQLHIYQLKTLIRMVKKTYRDFRLQGVLDSTLNSKTYETIRNRLTVEEATASVSEGGGLQGISMKDSDEEDEEDD |
| 预测分子量 | 152,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CADPS重组蛋白的参考文献概览：
1. **"Structural characterization of CADPS PH domain and its interaction with lipid membranes"**
- **作者**: Li X, et al.
- **摘要**: 通过重组表达纯化CADPS的pleckstrin homology (PH)结构域，利用X射线晶体学和脂质结合实验揭示了其与磷酸肌醇的相互作用机制，为理解CADPS在囊泡运输中的功能提供结构基础。
2. **"CADPS regulates exocytosis through Rab3A activation in neuroendocrine cells"**
- **作者**: Sato Y, et al.
- **摘要**: 研究利用重组CADPS蛋白进行体外GTPase激活实验，证明CADPS通过激活Rab3A调控神经内分泌细胞中激素分泌的分子通路，并发现其钙依赖性功能缺失与分泌异常相关。
3. **"Dysregulation of CADPS2 in autism spectrum disorder: Insights from recombinant protein rescue models"**
- **作者**: Chen R, et al.
- **摘要**: 在患者来源的神经元模型中，重组CADPS2蛋白恢复了突触囊泡释放缺陷，表明CADPS2基因突变通过损害分泌途径参与自闭症病理，提出靶向治疗的潜在方向。
注：以上文献信息为示例性质，实际引用需核对真实来源。如需具体文章，建议通过PubMed/Google Scholar检索关键词“CADPS recombinant protein”获取最新研究。
**Background of CADPS Recombinant Protein**
CADPS (Calcium-dependent secretion activator), also known as CAPS, is a critical regulator of calcium-dependent exocytosis, particularly in neurotransmitter and neuropeptide release. It facilitates vesicle priming and fusion by interacting with components of the secretory machinery, such as SNARE proteins and phosphatidylinositol lipids. CADPS is highly expressed in neuronal and endocrine cells, playing a pivotal role in synaptic transmission and hormone secretion.
Recombinant CADPS proteins are engineered *in vitro* using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional versions of the protein for research and therapeutic applications. These proteins retain key domains, including the C2 domain (calcium-binding) and PH domain (lipid interaction), enabling studies on vesicle dynamics, synaptic plasticity, and secretion mechanisms.
Research on CADPS recombinant proteins has advanced understanding of neurodevelopmental disorders, neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), and endocrine dysfunctions linked to secretion defects. Additionally, they serve as tools for drug screening or gene therapy targeting secretion pathways. Challenges include maintaining post-translational modifications and native conformation, often addressed via mammalian expression systems or fusion tags (e.g., His-tag).
Overall, CADPS recombinant proteins bridge mechanistic studies and translational applications, offering insights into cellular communication and therapeutic strategies for secretion-related pathologies.
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