| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TECTb |
| Uniprot No | Q96PL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304aa |
| 氨基酸序列 | MVTKAFVLLA IFAEASAKSC APNKADVILV FCYPKTIITK IPECPYGWEV HQLALGGLCY NGVHEGGYYQ FVIPDLSPKN KSYCGTQSEY KPPIYHFYSH IVSNDTTVIV KNQPVNYSFS CTYHSTYLVN QAAFDQRVAT VHVKNGSMGT FESQLSLNFY TNAKFSIKKE APFVLEASEI GSDLFAGVEA KGLSIRFKVV LNSCWATPSA DFMYPLQWQL INKGCPTDET VLVHENGRDH RATFQFNAFR FQNIPKLSKV WLHCETFICD SEKLSCPVTC DKRKRLLRDQ TGGVLVVELS LRSR |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TECTβ(Tectorin Beta)重组蛋白的示例文献(注：文献信息为模拟示例，具体内容需通过学术数据库验证)：
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1. **文献名称**: *Tectorin Beta重组蛋白在耳蜗机械传导中的功能研究*
**作者**: Chen et al., 2015
**摘要**: 本研究通过大肠杆菌表达系统成功获得高纯度TECTβ重组蛋白，并利用体外模型证明其在耳蜗盖膜结构中的关键作用，揭示了其通过调控机械敏感性离子通道影响听觉信号转导的分子机制。
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2. **文献名称**: *重组TECTβ蛋白的晶体结构解析及耳聋相关突变分析*
**作者**: Smith & Lee, 2018
**摘要**: 作者通过X射线衍射技术解析了重组人源TECTβ蛋白的三维结构，结合遗传学数据分析了导致常染色体隐性耳聋的TECTβ突变位点，为理解其致病机制提供了结构生物学依据。
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3. **文献名称**: *基于哺乳动物细胞系的TECTβ重组蛋白糖基化修饰研究*
**作者**: Wang et al., 2020
**摘要**: 研究利用HEK293细胞表达系统获得糖基化修饰的TECTβ重组蛋白，发现其糖链修饰对维持盖膜弹性和声波传导功能至关重要，非糖基化形式则显著降低体外模型的机械响应。
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**建议**：
- 实际文献检索可通过 **PubMed** 或 **Google Scholar** 使用关键词 "Recombinant TECTb protein" 或 "Tectorin Beta expression" 获取最新研究。
- 需注意基因命名规范(如TECTB可能为官方符号)，避免拼写差异影响检索结果。
**Background of TECTb Recombinant Protein**
TECTb, or tectorin beta, is a key extracellular matrix protein predominantly found in the tectorial membrane of the inner ear, a structure critical for auditory signal transduction. Encoded by the *TECTB* gene in humans, it plays a vital role in cochlear function by maintaining the mechanical properties of the tectorial membrane, which facilitates sound wave transmission and hair cell stimulation. Mutations in *TECTB* or related genes are linked to hereditary hearing loss, underscoring its importance in auditory physiology.
Structurally, TECTb contains multiple conserved domains, including zona pellucida (ZP) domains, which mediate protein polymerization and interaction with other cochlear components like α-tectorin. Recombinant TECTb protein is engineered using expression systems (e.g., mammalian cells or bacteria) to produce purified, functional forms for research. Its production enables studies on cochlear development, mechanotransduction mechanisms, and pathogenesis of hearing disorders.
In disease contexts, TECTb variants are associated with autosomal dominant nonsyndromic hearing loss (ADNSHL) and Usher syndrome. Recombinant TECTb serves as a tool to investigate these mutations' effects on protein structure, stability, and interaction networks. Additionally, it aids in developing diagnostic assays, gene therapies, or biomaterials for hearing restoration.
Despite progress, challenges remain in replicating TECTb's native post-translational modifications and tertiary structure *in vitro*. Ongoing research focuses on optimizing expression systems and elucidating TECTb's role in broader auditory pathways, offering insights into novel therapeutic strategies for hearing impairment.
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