| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACOX2 |
| Uniprot No | Q99424 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-681aa |
| 氨基酸序列 | MGSPVHRVSL GDTWSRQMHP DIESERYMQS FDVERLTNIL DGGAQNTALR RKVESIIHSY PEFSCKDNYF MTQNERYKAA MRRAFHIRLI ARRLGWLEDG RELGYAYRAL SGDVALNIHR VFVRALRSLG SEEQIAKWDP LCKNIQIIAT YAQTELGHGT YLQGLETEAT YDAATQEFVI HSPTLTATKW WPGDLGRSAT HALVQAQLIC SGARRGMHAF IVPIRSLQDH TPLPGIIIGD IGPKMDFDQT DNGFLQLNHV RVPRENMLSR FAQVLPDGTY VKLGTAQSNY LPMVVVRVEL LSGEILPILQ KACVIAMRYS VIRRQSRLRP SDPEAKVLDY QTQQQKLFPQ LAISYAFHFL AVSLLEFFQH SYTAILNQDF SFLPELHALS TGMKAMMSEF CTQGAEMCRR ACGGHGYSKL SGLPSLVTKL SASCTYEGEN TVLYLQVARF LVKSYLQTQM SPGSTPQRSL SPSVAYLTAP DLARCPAQRA ADFLCPELYT TAWAHVAVRL IKDSVQHLQT LTQSGADQHE AWNQTTVIHL QAAKVHCYYV TVKGFTEALE KLENEPAIQQ VLKRLCDLHA IHGILTNSGD FLHDAFLSGA QVDMARTAYL DLLRLIRKDA ILLTDAFDFT DQCLNSALGC YDGNVYERLF QWAQKSPTNT QENPAYEEYI RPLLQSWRSK L |
| 预测分子量 | 76,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACOX2重组蛋白的3篇参考文献摘要概括：
1. **"Functional characterization of human ACOX2 mutations in bile acid synthesis"**
- **作者**：Ferdinandusse S, et al.
- **摘要**：该研究通过重组表达人源ACOX2蛋白，分析了其在支链脂肪酸氧化中的作用，发现ACOX2突变会导致过氧化物酶体功能紊乱，与胆汁酸合成异常及肝病相关。
2. **"Recombinant ACOX2 enzyme activity assay and its role in metabolic disorders"**
- **作者**：Houten SM, et al.
- **摘要**：文章开发了重组ACOX2蛋白的体外活性检测方法，揭示了其在胆汁酸代谢中的关键功能，并探讨了ACOX2缺陷与遗传性代谢疾病的关联性。
3. **"Structural insights into ACOX2-mediated peroxisomal β-oxidation"**
- **作者**：Miyazawa S, et al.
- **摘要**：通过重组ACOX2蛋白的晶体结构分析，阐明了其底物结合域和催化机制，为理解其特异性降解分支链脂肪酸的分子基础提供了依据。
这些文献聚焦于ACOX2重组蛋白的功能分析、疾病关联及结构研究，适用于酶学或代谢疾病领域参考。
ACOX2 (Acyl-CoA Oxidase 2) is a peroxisomal enzyme critical for the β-oxidation of branched-chain fatty acids and bile acid intermediates. It catalyzes the first step in these pathways, converting specific acyl-CoA derivatives into trans-2-enoyl-CoA while transferring electrons to molecular oxygen, generating hydrogen peroxide. Structurally, ACOX2 belongs to the acyl-CoA oxidase family and shares homology with other isoforms (ACOX1. ACOX3), but exhibits distinct substrate specificity, preferentially metabolizing pristanoyl-CoA and dihydroxycholestanoyl-CoA.
Disruptions in ACOX2 function are linked to rare autosomal recessive disorders, such as peroxisomal biogenesis defects or specific bile acid synthesis errors, leading to metabolic imbalances, hepatic dysfunction, or neurological impairments. Research on recombinant ACOX2 proteins has advanced understanding of its enzymatic mechanisms, substrate interactions, and role in lipid homeostasis.
Recombinant ACOX2 is typically produced using heterologous expression systems (e.g., E. coli, mammalian cells) with affinity tags for purification. This engineered protein enables in vitro studies, including enzyme kinetics, inhibitor screening, and structural analyses (e.g., crystallography). It also aids in developing diagnostic tools for peroxisomal disorders and exploring therapeutic strategies, such as enzyme replacement or gene therapy. Recent studies highlight its potential in modeling metabolic diseases and refining precision medicine approaches.
Overall, ACOX2 recombinant proteins serve as vital tools for dissecting peroxisomal metabolism and bridging translational research in lipid-related pathologies.
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