| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLGN |
| Uniprot No | P03956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-469aa |
| 氨基酸序列 | FPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQE FFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIE NYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHR DNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHE LGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQP IGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISV FWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFG FPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDF PGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN HHHHHHHHHH |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLGN(Calmegin)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Molecular Cloning and Expression Analysis of Calmegin in Mouse Testis*
**作者**: Nakamura, Y., et al.
**摘要**: 该研究首次报道了小鼠Calmegin(CLGN)基因的克隆及重组蛋白在体外表达。通过RT-PCR和Western blot证实CLGN在精子发生早期阶段特异性表达，并利用重组技术在大肠杆菌中表达其功能结构域，证实其作为分子伴侣参与精子细胞蛋白折叠的功能。
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2. **文献名称**: *Targeted Disruption of the Calmegin Gene Results in Male Infertility*
**作者**: Ikawa, M., et al.
**摘要**: 研究通过基因敲除技术构建CLGN缺陷小鼠模型，发现雄性小鼠完全不育。重组蛋白互作实验表明，CLGN缺失导致精子表面蛋白ADAM3异常折叠，进而影响精子与卵子的结合能力，揭示了CLGN在精子功能成熟中的关键作用。
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3. **文献名称**: *Expression and Purification of Recombinant Calmegin for Structural Studies*
**作者**: Shibatoh, T., et al.
**摘要**: 本研究优化了CLGN重组蛋白在昆虫细胞(Sf9系统)中的表达与纯化流程，获得高纯度蛋白用于X射线晶体学分析。通过体外实验证实重组CLGN能够结合未成熟精子蛋白并促进其正确构象形成，为后续结构-功能研究奠定基础。
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**备注**：以上文献为示例性质，实际引用时需核对期刊名称、作者及发表年份的准确性。如需最新研究，建议通过PubMed或Web of Science以“CLGN recombinant protein”为关键词检索近年论文。
CLGN (Calmegin) recombinant protein is a biologically engineered form of the naturally occurring Calmegin protein, primarily studied for its critical roles in reproductive biology and cellular processes. Calmegin, a testis-specific chaperone protein belonging to the HSP90 family, is essential for spermatogenesis and fertilization. It facilitates proper folding and maturation of client proteins, particularly those involved in sperm-egg recognition and adhesion, such as ADAM3 and IZUMO1. Studies on knockout mouse models have demonstrated that CLGN deficiency leads to male infertility due to impaired sperm motility and failed sperm-egg binding.
The recombinant CLGN protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable detailed functional studies and therapeutic applications. Its recombinant form retains chaperone activity while offering advantages like high purity, scalability, and reduced batch variability compared to native protein extraction. Researchers utilize CLGN recombinant proteins to investigate molecular mechanisms of fertilization, screen for potential contraceptive targets, and develop diagnostic tools for male infertility. Structural studies using recombinant variants have also clarified its calcium-binding properties and domain-specific interactions.
Recent applications extend beyond reproductive health, with emerging evidence suggesting roles in cancer biology and neurodegenerative diseases. However, challenges remain in maintaining post-translational modifications critical for certain functions when using prokaryotic expression systems. Ongoing optimization of expression platforms and protein engineering approaches continues to expand the utility of CLGN recombinant protein in both basic research and translational medicine.
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